Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Se você estiver iniciando com células cultivadas ou amostras de tecidos, ou trabalhando com plantas, bactérias ou células de mamíferos, os kits e reagentes de extração de RNA Ambion oferecem o produto certo para sua pesquisa. A marca Ambion inclui o legado dos produtos Invitrogen, como kits de purificação confiáveis,TRIzol Reagents juntamente PureLink com os principais produtos da Ambion, como os kits e reagentes Cells-to-CT, MagMAX, e RNAlater
Order now | Order now | Order now | Order now | Order now | Order now | |
Optimized for liquid samples | Specialized kit deplete non-nucleated erythrocytes | Filter plate format with 96 columns, for HTP needs | HTP optimized for purification from stabilized blood samples | HTP format,capable of recovering RNA of all sizes (including microRNAs) | High RNA yield directly from whole blood, no need to isolate WBCs | |
---|---|---|---|---|---|---|
TRIzol LS | PureLink Total RNA Blood Kit | PureLink Pro 96 total RNA Purification Kit | MagMAX for Stabilized Blood Tubes RNA Isolation Kit | MagMAX mirVana Total RNA Isolation | RiboPure Blood Kit | |
Top Seller | ✓ | |||||
Compatible stabilization reagents | EDTA, Heparin, Citrate, RNAlater | EDTA, Heparin, Citrate | EDTA, Heparin, Citrate | Tempus or PaxGene tubes | EDTA or Citrate | EDTA, Heparin, Citrate, RNAlater |
Stabilization reagent included | ✓ RNAlater | |||||
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Fast, convenient silica columns in a plate format | Scalable, flexible format with magnetic beads | Scalable, flexible format with magnetic beads | Highest purity & convenience; includes both organic extraction & silica column (no precipitation required) |
Prep time | 1 hour | 20 minutes | 12 tubes in 2 hours | 96 samples in <1 hr | 30 minutes | |
High throughput compatible | ✓ | ✓ | ✓ |
For more info on working with blood please review our technical articles:
Purifying RNA from cultured cells has traditionally been a highly repetitive, time- and labor-intensive procedure, often involving the use of toxic organic chemicals. The products developed by our RNA experts allow you to isolate RNA of maximum yield, purity, and integrity using several technology formats (organic extraction, glass-fiber columns, magnetic beads, and combinations of these).
You can also analyze cultured cell lysates without first isolating the RNA. In significantly less time, you’ll get qPCR results equivalent to - or in some cases better than - those from purified RNA. The Invitrogen products such as Cells-to-CT and Single Cell-to-CT product lines offer tremendous performance and handling advantages.
Order now | Order now | Order now | Order now | Order now | Order now | |
Highly pure, intact RNA | Fast and convenient column based format for RNA (>200 nt) | Fast and convenient column based, filter plateformat for 96 samples (Rna > 200 nt) | Fast and convenient column based method from limited sample input (Rna >200 nt) | Fast and convenient method for small RNA species | Complete system for cells to qRT-PCR without RNA purification | |
TRIzol Reagent | PureLink RNA Mini Kit | PureLink Pro 96 total RNA Purification Kit | PureLink RNA Micro Scale Kit | MirVana miRNA Isolation Kit | Cells-to-CT kits | |
Top seller | ✓ | ✓ | ✓ | ✓ | ||
Prep time | 1 hour | 20 minutes | 20 minutes | 20 minutes | 30 minutes | <10 minutes |
Cell lysis method | High purity organic extraction (requires alcohol precipi-tation) | Nontoxic guanidine-isothio-cyanate lysis | Nontoxic guanidine-isothio-cyanate lysis | Nontoxic guanidine-isothio-cyanate lysis | Organic extraction followed by alcohol precipitation | Cells-to-CT lysis |
Amount of Starting material | Up to 1x107 cells | 5x106 to 1x108 cells | 5x106 to 1x108 cells | Up to 5x105 cells | 102-107 cultured cells | 10-100,000 cells |
Appli-cation | All RNA expression methods | qRT-PCR, NGS | qRT-PCR, NGS | qRT-PCR, NGS | Micro RNA analysis | qRT-PCR |
RNAlater Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.
Order now | Order now | |
Low-throughput isolation of RNA and DNA from the same FFPE section | Scalable isolation of RNA and DNA from the same FFPE section | |
---|---|---|
RecoverAll Total Nucleic Acid Isolation Kit for FFPE | MagMAX FFPE DNA/RNA Ultra Kit | |
Prep Time | 4.5 hours (total time for 16 samples) 2.5 hrs hands-on time | 4 hours (total time for 96 preps) 1 hr hands-on time |
Final product extracted | total RNA, microRNA, gDNA | total RNA, microRNA, gDNA |
Isolation method | column purification | mag bead purification |
Deparaffinization required | ✓ | ✓ |
Automation compatible (post deparffinization) | ✓ (Scripts for KF Flex and Duo Prime) | |
Compatible FFPE sample range | up to 4 x 20 micron sections (300 mm2 tissue) | up to 2 x 20 micron sections (300 mm2 tissue) |
Sample volume | ~50µL purified DNA ~50µL purified RNA | ~50uL purified DNA ~50µL purified RNA |
Compatible with Ion AmpliSeq DNA panels (e.g. Cancer Hotspot Panel v2) | ✓ | ✓ |
Compatible with Ion AmpliSeq RNA panels (e.g. RNA cancer and RNA fusion) | ✓ | ✓ |
The recovery of quality RNA from FFPE specimens can be quite challenging. The fixation process causes cross-linkage between nucleic acids and proteins, and covalently modifies RNA by the addition of monomethyl groups to the bases. As a result, the molecules are rigid and susceptible to mechanical shearing, and the cross-links may compromise the use of RNA as a substrate for reverse transcription. Therefore, in order to utilize FFPE tissues as a source for gene expression analysis, a reliable method is required for extraction of RNA from the cross-linked matrix.
There are a number of factors that can impact the overall quality and yield of RNA isolated from FFPE tissues. Here are recommendations to address several key factors:
The MagMAX FFPE DNA/RNA Ultra Kits are designed for rapid, efficient, and automatable isolation of total RNA and DNA from FFPE samples using Invitrogen MagMAX magnetic particles. Invitrogen MagMAX FFPE NA kits offer faster workflows and use less toxic reagents, without sacrificing quality in the process. These kits help deliver nucleic acid yield and purity comparable to the best-in-class RecoverAll filter-based system. The MagMAX FFPE NA kits outperform products with similar protocols, and achieve RNA and DNA yields that are comparable with longer, more hazardous, and lower-throughput protocols.
The RecoverAll Total Nucleic Acid Isolation Kit procedure requires about 45 minutes of hands-on time and can easily be completed in less than 1 day when isolating RNA. FFPE samples are deparaffinized using a series of xylene and ethanol washes. Next, they are subjected to a rigorous protease digestion with an incubation time tailored for recovery of either RNA or DNA. The nucleic acids are purified using a rapid glass-filter methodology that includes an on-filter nuclease treatment, and are eluted into either water or the low-salt buffer provided.
TechNotes Articles
Tips from the Bench
Your Data
Application Note
Product Bulletin
Order now | Order now | Order now | Order now | |
Great for difficult samples (conifer tissue & seeds) | Quick & easy to use | Efficient recovery of microRNA & small RNA | Rapid & fully automated | |
---|---|---|---|---|
Plant RNA Reagent | PureLink RNA Mini Kit | mirVana miRNA Isolation Kit | MagMAX-96 Total RNA Isolation Kit | |
Top seller | ✓ | |||
RNA types isolated | large RNA molecules only | large RNA molecules only | Small & large RNA molecules | large RNA molecules only |
Prep time | 60 minutes | <20 minutes | 30 minutes | <45 minutes |
Amount of starting material | Up to 1 g | <250 mg | 0.5 to 250 mg | Up to 10 mg |
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Highest purity & convenience; includes organic extraction & silica column (no precipitation required) | Scalable, flexible format with magnetic beads |
High throughput compatible | ✓ | |||
Compatible products | Ambion Plant RNA Isolation Aid Removes common plant contaminants | Ambion Plant RNA Isolation Aid Removes common plant contaminants | Ambion Plant RNA Isolation Aid Removes common plant contaminants |
One of the major issues with plant RNA isolation is the presence of problematic biomolecules, such as polyphenolic compounds and polysaccharides. The Ambion Plant RNA Isolation Aid minimizes many of these contaminants with the addition of polyvinylpyrrolidone, a high molecular weight polymer that binds to polyphenolics and polysaccharides and a pre-extraction spin step.
RNAlater Solution is widely for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It minimizes the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.
Order now | Order now | Order now | Order now | Order now | Order now | Order now | |
Gold standard for highly pure, intact RNA | Simple, reliable, rapid method | Fast and convenient column based, filter plateformat for 96 samples (Rna > 200 nt) | Fast and convenient column based method from limited sample input (Rna >200 nt) | Fast and convenient method for small RNA species | Highest purity TRIzol + column purification, no precipitation required | HTP format, capable of recovering RNA of all sizes (including microRNAs) | |
---|---|---|---|---|---|---|---|
TRIzol Reagent | PureLink RNA Mini Kit | PureLink Pro 96 total RNA Purification Kit | PureLink RNA Micro Scale Kit | mirVana miRNA Isolation Kit | TRIzol Plus RNA Purification System | MagMAX mirVana Total RNA Isolation | |
Top Seller | ✓ | ||||||
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Fast Convenient silica column in a filter plate format | Fast Convenient micro silica columns | Organic extraction combined with convenient silica columns | Highest purity and convenience; includes both organic extraction and silica column (no precipitation required) | Scalable, flexible format with magnetic beads |
Prep time | 1 hour | 20 minutes | 20 minutes | 20 minutes | 30 minutes | 1 hour | <1 hour |
Amount of starting material | Up to 100 mg | 10 mg to 100 mg | 10 cells to 10 mg | up to 10 mg | up to 250 mg tissue | Up to 100 mg | Up to 50 mg |
High throughput compatible | ✓ | ✓ | |||||
Prep size | 100 preps | 50 preps | 96 preps | 50 preps | 96 preps | 50 preps | 96 preps |
RNA content can vary widely between tissues, cell types, physiological states, etc. This chart provides general guidelines for estimating RNA yields from a variety of cells and tissues.
Table 1. Typical total RNA yields from 1 mg of various tissues.
RNAlater Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.
Order now | Order now | Order now | Order now | |
Rapid & fully automated | Quick & easy to use | Designed for rigid yeast cell walls while maintaining expression profiles | Fast and convenient column based, filter plateformat for 96 samples (Rna > 200 nt) | |
---|---|---|---|---|
Platinum Taq MagMAX for Microarrays Total RNA Isolation Kit | PureLink RNA Mini Kit | RiboPure-Yeast Kit | PureLink Pro 96 total RNA Purification Kit | |
Top seller | ✓ | |||
Includes specialized beads for cell lysis | No | No | Zirconia beads | No |
Isolation method | Scalable, flexible format for highest purity; includes organic extraction and magnetic beads | Fast, convenient silica column | Highest purity and convenience; includes both organic extraction and silica column | Fast, convenient silica column in 96 well filter plate format |
Prep time | <1 hour | 20 minutes | 90 minutes (includes DNase treatment) | 20 minutes |
Amount of starting material | Up to 5 x 106 cells | Up to 5 x 108 cells | Up to 3 x 108 cells | 1.8 mL of fresh, log-phase yeast cells (OD660=0.6–0.8) |
High throughput compatible | ✓ | |||
Kit size | 96 preps | 50 preps | 50 preps | 96 preps |
Unlike eukaryotic cells, yeast and many bacterial strains are protected by cell walls that can be difficult to disrupt. As a first step, effective cell lysis (typically via mechanical disruption or enzymatic pretreatment) is required prior to any RNA isolation procedure.
A range of products developed by the Ambion RNA experts will help you isolate high yields of pure, intact RNA from bacteria and yeast.
Order now | Order now | Order now | Order now | Order now | |
No lysis procedures | Fast & easy, 20 min | Fast and convenient silica column based , filter plate format | HTP, 45 min | Ideal for hard-to-lyse gram + bacteria | |
---|---|---|---|---|---|
TRIzol Max Bacterial RNA Isolation Kit w/TRIzol & Max Bacterial Enhancement Reagent | PureLink RNA Mini Kit | PureLink Pro 96 total RNA Purification Kit | MagMAX Total Nucleic Acid Isolation Kit | RiboPure-Bacteria Kit | |
Top Seller | ✓ | ||||
Includes specialized beads for cell lysis | No | No | No | Zirconia beads | Zirconia beads |
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Fast, convenient silica column in 96 well filter plate format | Scalable, flexible format with magnetic beads | Highest purity & convenience; includes organic extraction & silica column (no precipitation required) |
Prep time | 1 hour | 20 minutes | 20 minutes | 45 minutes | 1 hour |
Amount of starting material | Up to 1 x 108 cells | Up to 1 x 109 cells | Up to 1 x 109 cells | Up to 0.3 g solid samples or 175 µl liquid samples | Up to 109 cells |
High throughput compatible | ✓ | ✓ | |||
Kit size | 100 preps | 50 preps | 96 preps | 100 preps | 50 preps |
Viral analysis of biological (animal, insect, plant, fungal, bacterial) and environmental (water, air, food) samples is increasingly conducted using molecular testing strategies that require viral RNA or DNA from the samples being tested. Purification of viral RNA poses unique challenges for getting good recovery and detection sensitivity. Ambion RNA experts have developed RNA purification products that are optimized to provide maximum viral RNA yield, purity, and integrity from a broad range of sample types in several format options.
"Highly concentrated viral RNA from the PureLink Viral RNA/DNA Mini Kit provides a tool for more sensitive and delicate downstream analysis on viral genomes”
Most Suitable Downstream Application | ||||||
---|---|---|---|---|---|---|
Product | Format | Real-Time RT-PCR | Microarray Analysis | Sequen-cing | Northern Blot | Cloning |
Total RNA | ||||||
PureLink Viral RNA⁄DNA Mini Kit | Chaotropic salt-based lysis, silica filter | ✓ | ✓ | |||
RNAqueous Kit | Chaotropic salt-based lysis, silica filter | ✓ | ✓ | ✓ | ||
MagMAX AI⁄ND Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles | ✓ | ✓ | |||
MagMAX-96 AI⁄ND Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ✓ | |||
DNA/RNA | ||||||
PureLink Pro 96 Viral RNA⁄DNA Purification Kit | Chaotropic salt-based lysis, silica filter, 96-well processing, automation compatible | ✓ | ||||
MagMAX Total Nucleic Acid Isolation Kit | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ✓ | |||
MagMAX-96 Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ✓ | |||
Dynabeads SILANE Viral NA | Chaotropic salt-based lysis, magnetic beads, automation compatible | |||||
iPrep PureLink Virus Kit | Chaotropic salt-based lysis, magnetic particles, automation compatible | ✓ | ||||
MagMAX Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles | ✓ | ||||
MagMAX-96 Viral RNA Isolation Kit (5 x 96 rxns) | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ||||
MicroRNA | ||||||
mirVana miRNA Isolation Kit | Chaotropic salt-based lysis, silica filter | ✓ | ||||
mirVana miRNA Isolation Kit (without phenol - must be purchased separately) | Chaotropic salt-based lysis, silica filter | ✓ | ||||
TRIzol LS Reagent | Lysis in TRIzol reagent, one-step reagent | ✓ | ✓ | ✓ | ✓ | |
mRNA | ||||||
Dynabeads mRNA DIRECT Kit | Chaotropic salt-based lysis, magnetic beads | ✓ | ✓ | |||
Dynabeads mRNA DIRECT Micro Kit | Chaotropic salt-based lysis, magnetic beads | ✓ | ✓ |
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
Order now | Order now | Order now | Order now | Order now | Order now | |
Optimized for liquid samples | Specialized kit deplete non-nucleated erythrocytes | Filter plate format with 96 columns, for HTP needs | HTP optimized for purification from stabilized blood samples | HTP format,capable of recovering RNA of all sizes (including microRNAs) | High RNA yield directly from whole blood, no need to isolate WBCs | |
---|---|---|---|---|---|---|
TRIzol LS | PureLink Total RNA Blood Kit | PureLink Pro 96 total RNA Purification Kit | MagMAX for Stabilized Blood Tubes RNA Isolation Kit | MagMAX mirVana Total RNA Isolation | RiboPure Blood Kit | |
Top Seller | ✓ | |||||
Compatible stabilization reagents | EDTA, Heparin, Citrate, RNAlater | EDTA, Heparin, Citrate | EDTA, Heparin, Citrate | Tempus or PaxGene tubes | EDTA or Citrate | EDTA, Heparin, Citrate, RNAlater |
Stabilization reagent included | ✓ RNAlater | |||||
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Fast, convenient silica columns in a plate format | Scalable, flexible format with magnetic beads | Scalable, flexible format with magnetic beads | Highest purity & convenience; includes both organic extraction & silica column (no precipitation required) |
Prep time | 1 hour | 20 minutes | 12 tubes in 2 hours | 96 samples in <1 hr | 30 minutes | |
High throughput compatible | ✓ | ✓ | ✓ |
For more info on working with blood please review our technical articles:
Purifying RNA from cultured cells has traditionally been a highly repetitive, time- and labor-intensive procedure, often involving the use of toxic organic chemicals. The products developed by our RNA experts allow you to isolate RNA of maximum yield, purity, and integrity using several technology formats (organic extraction, glass-fiber columns, magnetic beads, and combinations of these).
You can also analyze cultured cell lysates without first isolating the RNA. In significantly less time, you’ll get qPCR results equivalent to - or in some cases better than - those from purified RNA. The Invitrogen products such as Cells-to-CT and Single Cell-to-CT product lines offer tremendous performance and handling advantages.
Order now | Order now | Order now | Order now | Order now | Order now | |
Highly pure, intact RNA | Fast and convenient column based format for RNA (>200 nt) | Fast and convenient column based, filter plateformat for 96 samples (Rna > 200 nt) | Fast and convenient column based method from limited sample input (Rna >200 nt) | Fast and convenient method for small RNA species | Complete system for cells to qRT-PCR without RNA purification | |
TRIzol Reagent | PureLink RNA Mini Kit | PureLink Pro 96 total RNA Purification Kit | PureLink RNA Micro Scale Kit | MirVana miRNA Isolation Kit | Cells-to-CT kits | |
Top seller | ✓ | ✓ | ✓ | ✓ | ||
Prep time | 1 hour | 20 minutes | 20 minutes | 20 minutes | 30 minutes | <10 minutes |
Cell lysis method | High purity organic extraction (requires alcohol precipi-tation) | Nontoxic guanidine-isothio-cyanate lysis | Nontoxic guanidine-isothio-cyanate lysis | Nontoxic guanidine-isothio-cyanate lysis | Organic extraction followed by alcohol precipitation | Cells-to-CT lysis |
Amount of Starting material | Up to 1x107 cells | 5x106 to 1x108 cells | 5x106 to 1x108 cells | Up to 5x105 cells | 102-107 cultured cells | 10-100,000 cells |
Appli-cation | All RNA expression methods | qRT-PCR, NGS | qRT-PCR, NGS | qRT-PCR, NGS | Micro RNA analysis | qRT-PCR |
RNAlater Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.
Order now | Order now | |
Low-throughput isolation of RNA and DNA from the same FFPE section | Scalable isolation of RNA and DNA from the same FFPE section | |
---|---|---|
RecoverAll Total Nucleic Acid Isolation Kit for FFPE | MagMAX FFPE DNA/RNA Ultra Kit | |
Prep Time | 4.5 hours (total time for 16 samples) 2.5 hrs hands-on time | 4 hours (total time for 96 preps) 1 hr hands-on time |
Final product extracted | total RNA, microRNA, gDNA | total RNA, microRNA, gDNA |
Isolation method | column purification | mag bead purification |
Deparaffinization required | ✓ | ✓ |
Automation compatible (post deparffinization) | ✓ (Scripts for KF Flex and Duo Prime) | |
Compatible FFPE sample range | up to 4 x 20 micron sections (300 mm2 tissue) | up to 2 x 20 micron sections (300 mm2 tissue) |
Sample volume | ~50µL purified DNA ~50µL purified RNA | ~50uL purified DNA ~50µL purified RNA |
Compatible with Ion AmpliSeq DNA panels (e.g. Cancer Hotspot Panel v2) | ✓ | ✓ |
Compatible with Ion AmpliSeq RNA panels (e.g. RNA cancer and RNA fusion) | ✓ | ✓ |
The recovery of quality RNA from FFPE specimens can be quite challenging. The fixation process causes cross-linkage between nucleic acids and proteins, and covalently modifies RNA by the addition of monomethyl groups to the bases. As a result, the molecules are rigid and susceptible to mechanical shearing, and the cross-links may compromise the use of RNA as a substrate for reverse transcription. Therefore, in order to utilize FFPE tissues as a source for gene expression analysis, a reliable method is required for extraction of RNA from the cross-linked matrix.
There are a number of factors that can impact the overall quality and yield of RNA isolated from FFPE tissues. Here are recommendations to address several key factors:
The MagMAX FFPE DNA/RNA Ultra Kits are designed for rapid, efficient, and automatable isolation of total RNA and DNA from FFPE samples using Invitrogen MagMAX magnetic particles. Invitrogen MagMAX FFPE NA kits offer faster workflows and use less toxic reagents, without sacrificing quality in the process. These kits help deliver nucleic acid yield and purity comparable to the best-in-class RecoverAll filter-based system. The MagMAX FFPE NA kits outperform products with similar protocols, and achieve RNA and DNA yields that are comparable with longer, more hazardous, and lower-throughput protocols.
The RecoverAll Total Nucleic Acid Isolation Kit procedure requires about 45 minutes of hands-on time and can easily be completed in less than 1 day when isolating RNA. FFPE samples are deparaffinized using a series of xylene and ethanol washes. Next, they are subjected to a rigorous protease digestion with an incubation time tailored for recovery of either RNA or DNA. The nucleic acids are purified using a rapid glass-filter methodology that includes an on-filter nuclease treatment, and are eluted into either water or the low-salt buffer provided.
TechNotes Articles
Tips from the Bench
Your Data
Application Note
Product Bulletin
Order now | Order now | Order now | Order now | |
Great for difficult samples (conifer tissue & seeds) | Quick & easy to use | Efficient recovery of microRNA & small RNA | Rapid & fully automated | |
---|---|---|---|---|
Plant RNA Reagent | PureLink RNA Mini Kit | mirVana miRNA Isolation Kit | MagMAX-96 Total RNA Isolation Kit | |
Top seller | ✓ | |||
RNA types isolated | large RNA molecules only | large RNA molecules only | Small & large RNA molecules | large RNA molecules only |
Prep time | 60 minutes | <20 minutes | 30 minutes | <45 minutes |
Amount of starting material | Up to 1 g | <250 mg | 0.5 to 250 mg | Up to 10 mg |
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Highest purity & convenience; includes organic extraction & silica column (no precipitation required) | Scalable, flexible format with magnetic beads |
High throughput compatible | ✓ | |||
Compatible products | Ambion Plant RNA Isolation Aid Removes common plant contaminants | Ambion Plant RNA Isolation Aid Removes common plant contaminants | Ambion Plant RNA Isolation Aid Removes common plant contaminants |
One of the major issues with plant RNA isolation is the presence of problematic biomolecules, such as polyphenolic compounds and polysaccharides. The Ambion Plant RNA Isolation Aid minimizes many of these contaminants with the addition of polyvinylpyrrolidone, a high molecular weight polymer that binds to polyphenolics and polysaccharides and a pre-extraction spin step.
RNAlater Solution is widely for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It minimizes the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.
Order now | Order now | Order now | Order now | Order now | Order now | Order now | |
Gold standard for highly pure, intact RNA | Simple, reliable, rapid method | Fast and convenient column based, filter plateformat for 96 samples (Rna > 200 nt) | Fast and convenient column based method from limited sample input (Rna >200 nt) | Fast and convenient method for small RNA species | Highest purity TRIzol + column purification, no precipitation required | HTP format, capable of recovering RNA of all sizes (including microRNAs) | |
---|---|---|---|---|---|---|---|
TRIzol Reagent | PureLink RNA Mini Kit | PureLink Pro 96 total RNA Purification Kit | PureLink RNA Micro Scale Kit | mirVana miRNA Isolation Kit | TRIzol Plus RNA Purification System | MagMAX mirVana Total RNA Isolation | |
Top Seller | ✓ | ||||||
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Fast Convenient silica column in a filter plate format | Fast Convenient micro silica columns | Organic extraction combined with convenient silica columns | Highest purity and convenience; includes both organic extraction and silica column (no precipitation required) | Scalable, flexible format with magnetic beads |
Prep time | 1 hour | 20 minutes | 20 minutes | 20 minutes | 30 minutes | 1 hour | <1 hour |
Amount of starting material | Up to 100 mg | 10 mg to 100 mg | 10 cells to 10 mg | up to 10 mg | up to 250 mg tissue | Up to 100 mg | Up to 50 mg |
High throughput compatible | ✓ | ✓ | |||||
Prep size | 100 preps | 50 preps | 96 preps | 50 preps | 96 preps | 50 preps | 96 preps |
RNA content can vary widely between tissues, cell types, physiological states, etc. This chart provides general guidelines for estimating RNA yields from a variety of cells and tissues.
Table 1. Typical total RNA yields from 1 mg of various tissues.
RNAlater Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.
Order now | Order now | Order now | Order now | |
Rapid & fully automated | Quick & easy to use | Designed for rigid yeast cell walls while maintaining expression profiles | Fast and convenient column based, filter plateformat for 96 samples (Rna > 200 nt) | |
---|---|---|---|---|
Platinum Taq MagMAX for Microarrays Total RNA Isolation Kit | PureLink RNA Mini Kit | RiboPure-Yeast Kit | PureLink Pro 96 total RNA Purification Kit | |
Top seller | ✓ | |||
Includes specialized beads for cell lysis | No | No | Zirconia beads | No |
Isolation method | Scalable, flexible format for highest purity; includes organic extraction and magnetic beads | Fast, convenient silica column | Highest purity and convenience; includes both organic extraction and silica column | Fast, convenient silica column in 96 well filter plate format |
Prep time | <1 hour | 20 minutes | 90 minutes (includes DNase treatment) | 20 minutes |
Amount of starting material | Up to 5 x 106 cells | Up to 5 x 108 cells | Up to 3 x 108 cells | 1.8 mL of fresh, log-phase yeast cells (OD660=0.6–0.8) |
High throughput compatible | ✓ | |||
Kit size | 96 preps | 50 preps | 50 preps | 96 preps |
Unlike eukaryotic cells, yeast and many bacterial strains are protected by cell walls that can be difficult to disrupt. As a first step, effective cell lysis (typically via mechanical disruption or enzymatic pretreatment) is required prior to any RNA isolation procedure.
A range of products developed by the Ambion RNA experts will help you isolate high yields of pure, intact RNA from bacteria and yeast.
Order now | Order now | Order now | Order now | Order now | |
No lysis procedures | Fast & easy, 20 min | Fast and convenient silica column based , filter plate format | HTP, 45 min | Ideal for hard-to-lyse gram + bacteria | |
---|---|---|---|---|---|
TRIzol Max Bacterial RNA Isolation Kit w/TRIzol & Max Bacterial Enhancement Reagent | PureLink RNA Mini Kit | PureLink Pro 96 total RNA Purification Kit | MagMAX Total Nucleic Acid Isolation Kit | RiboPure-Bacteria Kit | |
Top Seller | ✓ | ||||
Includes specialized beads for cell lysis | No | No | No | Zirconia beads | Zirconia beads |
Isolation method | High purity organic extraction (requires alcohol precipitation) | Fast, convenient silica column | Fast, convenient silica column in 96 well filter plate format | Scalable, flexible format with magnetic beads | Highest purity & convenience; includes organic extraction & silica column (no precipitation required) |
Prep time | 1 hour | 20 minutes | 20 minutes | 45 minutes | 1 hour |
Amount of starting material | Up to 1 x 108 cells | Up to 1 x 109 cells | Up to 1 x 109 cells | Up to 0.3 g solid samples or 175 µl liquid samples | Up to 109 cells |
High throughput compatible | ✓ | ✓ | |||
Kit size | 100 preps | 50 preps | 96 preps | 100 preps | 50 preps |
Viral analysis of biological (animal, insect, plant, fungal, bacterial) and environmental (water, air, food) samples is increasingly conducted using molecular testing strategies that require viral RNA or DNA from the samples being tested. Purification of viral RNA poses unique challenges for getting good recovery and detection sensitivity. Ambion RNA experts have developed RNA purification products that are optimized to provide maximum viral RNA yield, purity, and integrity from a broad range of sample types in several format options.
"Highly concentrated viral RNA from the PureLink Viral RNA/DNA Mini Kit provides a tool for more sensitive and delicate downstream analysis on viral genomes”
Most Suitable Downstream Application | ||||||
---|---|---|---|---|---|---|
Product | Format | Real-Time RT-PCR | Microarray Analysis | Sequen-cing | Northern Blot | Cloning |
Total RNA | ||||||
PureLink Viral RNA⁄DNA Mini Kit | Chaotropic salt-based lysis, silica filter | ✓ | ✓ | |||
RNAqueous Kit | Chaotropic salt-based lysis, silica filter | ✓ | ✓ | ✓ | ||
MagMAX AI⁄ND Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles | ✓ | ✓ | |||
MagMAX-96 AI⁄ND Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ✓ | |||
DNA/RNA | ||||||
PureLink Pro 96 Viral RNA⁄DNA Purification Kit | Chaotropic salt-based lysis, silica filter, 96-well processing, automation compatible | ✓ | ||||
MagMAX Total Nucleic Acid Isolation Kit | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ✓ | |||
MagMAX-96 Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ✓ | |||
Dynabeads SILANE Viral NA | Chaotropic salt-based lysis, magnetic beads, automation compatible | |||||
iPrep PureLink Virus Kit | Chaotropic salt-based lysis, magnetic particles, automation compatible | ✓ | ||||
MagMAX Viral RNA Isolation Kit | Chaotropic salt-based lysis, magnetic particles | ✓ | ||||
MagMAX-96 Viral RNA Isolation Kit (5 x 96 rxns) | Chaotropic salt-based lysis, magnetic particles, 96-well processing, automation compatible | ✓ | ||||
MicroRNA | ||||||
mirVana miRNA Isolation Kit | Chaotropic salt-based lysis, silica filter | ✓ | ||||
mirVana miRNA Isolation Kit (without phenol - must be purchased separately) | Chaotropic salt-based lysis, silica filter | ✓ | ||||
TRIzol LS Reagent | Lysis in TRIzol reagent, one-step reagent | ✓ | ✓ | ✓ | ✓ | |
mRNA | ||||||
Dynabeads mRNA DIRECT Kit | Chaotropic salt-based lysis, magnetic beads | ✓ | ✓ | |||
Dynabeads mRNA DIRECT Micro Kit | Chaotropic salt-based lysis, magnetic beads | ✓ | ✓ |
Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.