High fidelity DNA polymerases are PCR enzymes that amplify target sequences accurately. These enzymes achieve high fidelity due to their proofreading ability, which correct misincorporated nucleotides during amplification. Invitrogen Platinum SuperFi II DNA Polymerase is a hot-start, engineered DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer specially formulated for primer annealing at 60°C, Platinum SuperFi II DNA Polymerase offers efficiency and simplicity in PCR applications requiring highest PCR accuracy, such as cloning, sequencing, and mutagenesis.

Highlights of Platinum SuperFi II DNA Polymerase

  • Exceptional accuracy—Higher than 300x Taq fidelity as determined by next-generation sequencing
  • Simplified workflow—Buffer formulated for primer annealing at 60°C (no need for a Tm calculator)
  • Increased PCR success—Robust amplification of GC-rich targets, DNA of suboptimal purity, and long sequences
  • Enabled automation—High specificity and benchtop stability for 24 hours after reaction setup, enabled by Invitrogen Platinum hot-start technology
  • Reduced pipetting—Master mix options available with or without direct gel-loading dyes

Watch the video to learn about the fidelity of a DNA polymerase, methods for measuring enzyme fidelity, and benefits of using a high-fidelity DNA polymerase in your PCR

Watch the benefits of Platinum SuperFi II DNA Polymerase.

Benefits of high fidelity Platinum SuperFi II DNA Polymerase

High fidelity

Platinum SuperFi II DNA Polymerase preserves DNA sequence accuracy with its extremely low error rate. Using next-generation sequencing, the relative fidelity of Platinum SuperFi II DNA polymerase was calculated to be >300x that of Taq DNA polymerase (Figure 1).

Bar graph showing Platinum SuperFi II DNA polymerase has the highest fidelity compared to Taq polymerase

Figure 1. Fidelity comparison across commercially available enzymes relative to Taq enzyme. A 3.9 kb sequence was amplified by PCR using different DNA polymerases, and the resulting PCR amplicons were then fragmented with a MuA transposase. Unique molecular identifiers (UMI), which consist of 12 random nucleotides, were introduced during fragmentation to individually tag each product. After next-generation sequencing, reads were aligned to the correct sequence, grouped by UMI families, and errors were called. Errors were identified only if they were present in all reads in the UMI family; otherwise, they were discarded as sequencing errors. The polymerase fidelities were normalized to the Taq DNA polymerase. 

Hibridização de primer a 60º C

The innovative buffer formulation of Platinum SuperFi II buffer enables annealing of primers at 60°C regardless of their sequences (Figure 2). The buffer also allows successful amplification when calculated Tms are used in the annealing step (data not shown). This universal annealing feature allows co-cycling assays (Figure 3 and 4).

Gel image with clear bands

Figure 2.Platinum SuperFi II DNA Polymerase produces PCR products with high specificity and yield following the universal annealing temperature at 60°C. Primer sets of varying annealing temperature were used to amplify 12 targets from 50 ng of human genomic DNA at 60°C annealing temperature. The molecular weight marker is Invitrogen TrackIt 1 Kb Plus DNA Ladder. The annealing temperatures stated were calculated using the Tm calculator for Platinum SuperFi DNA Polymerase.

Line diagram shows that three targets can be amplified simultaneously

Figure 3. Time saving and assay co-cycling enabled by universal PCR protocol. PCR assays using conventional PCR reagents require specific protocols for amplification of each DNA fragment because of the different primer annealing temperatures and extension steps; therefore, multiple targets often cannot be amplified together in the same PCR run. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using one protocol with a universal primer annealing temperature and the extension time selected for the longest fragment to be amplified (Figure 4).

Gel image with bands of short and long amplicons

Figure 4. Platinum SuperFi II DNA Polymerase enables cycling of shorter and longer amplicons together.  0.7 kb, 2.0 kb, 4.8 kb, and 14 kb fragments were amplified from 100 ng of human genomic DNA using the same protocol for all four targets: 98°C denaturation for 10 sec, 60°C annealing for 10 sec, 72°C extension for 7 min. The extension time was based on length of the longest target.

Carregamento direto no gel

Platinum SuperFi II Green PCR Master Mix offers the convenience of direct gel loading of PCR products, eliminating tedious steps of dye addition to PCR samples, and helping reduce pipetting errors. The green buffer (Figure 5) is compatible with downstream applications including DNA sequencing, ligation, and restriction digestion.

Schematic shows workflow sequence from tube of green mastermix and a target DNA to thermal cycler to colored electrophoretic bands

Figure 5. The green master mix format for loading PCR products directly to a gel for analysis. The master mix of Platinum SuperFi II DNA Polymerase is available in a green buffer format that contains a density reagent and two tracking dyes. DNA migration is easily tracked with two dyes (blue and yellow) that are readily visible during electrophoresis (the lanes for 5 and 15 min in the figure to the right).

High fidelity

Platinum SuperFi II DNA Polymerase preserves DNA sequence accuracy with its extremely low error rate. Using next-generation sequencing, the relative fidelity of Platinum SuperFi II DNA polymerase was calculated to be >300x that of Taq DNA polymerase (Figure 1).

Bar graph showing Platinum SuperFi II DNA polymerase has the highest fidelity compared to Taq polymerase

Figure 1. Fidelity comparison across commercially available enzymes relative to Taq enzyme. A 3.9 kb sequence was amplified by PCR using different DNA polymerases, and the resulting PCR amplicons were then fragmented with a MuA transposase. Unique molecular identifiers (UMI), which consist of 12 random nucleotides, were introduced during fragmentation to individually tag each product. After next-generation sequencing, reads were aligned to the correct sequence, grouped by UMI families, and errors were called. Errors were identified only if they were present in all reads in the UMI family; otherwise, they were discarded as sequencing errors. The polymerase fidelities were normalized to the Taq DNA polymerase. 

Hibridização de primer a 60º C

The innovative buffer formulation of Platinum SuperFi II buffer enables annealing of primers at 60°C regardless of their sequences (Figure 2). The buffer also allows successful amplification when calculated Tms are used in the annealing step (data not shown). This universal annealing feature allows co-cycling assays (Figure 3 and 4).

Gel image with clear bands

Figure 2.Platinum SuperFi II DNA Polymerase produces PCR products with high specificity and yield following the universal annealing temperature at 60°C. Primer sets of varying annealing temperature were used to amplify 12 targets from 50 ng of human genomic DNA at 60°C annealing temperature. The molecular weight marker is Invitrogen TrackIt 1 Kb Plus DNA Ladder. The annealing temperatures stated were calculated using the Tm calculator for Platinum SuperFi DNA Polymerase.

Line diagram shows that three targets can be amplified simultaneously

Figure 3. Time saving and assay co-cycling enabled by universal PCR protocol. PCR assays using conventional PCR reagents require specific protocols for amplification of each DNA fragment because of the different primer annealing temperatures and extension steps; therefore, multiple targets often cannot be amplified together in the same PCR run. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using one protocol with a universal primer annealing temperature and the extension time selected for the longest fragment to be amplified (Figure 4).

Gel image with bands of short and long amplicons

Figure 4. Platinum SuperFi II DNA Polymerase enables cycling of shorter and longer amplicons together.  0.7 kb, 2.0 kb, 4.8 kb, and 14 kb fragments were amplified from 100 ng of human genomic DNA using the same protocol for all four targets: 98°C denaturation for 10 sec, 60°C annealing for 10 sec, 72°C extension for 7 min. The extension time was based on length of the longest target.

Carregamento direto no gel

Platinum SuperFi II Green PCR Master Mix offers the convenience of direct gel loading of PCR products, eliminating tedious steps of dye addition to PCR samples, and helping reduce pipetting errors. The green buffer (Figure 5) is compatible with downstream applications including DNA sequencing, ligation, and restriction digestion.

Schematic shows workflow sequence from tube of green mastermix and a target DNA to thermal cycler to colored electrophoretic bands

Figure 5. The green master mix format for loading PCR products directly to a gel for analysis. The master mix of Platinum SuperFi II DNA Polymerase is available in a green buffer format that contains a density reagent and two tracking dyes. DNA migration is easily tracked with two dyes (blue and yellow) that are readily visible during electrophoresis (the lanes for 5 and 15 min in the figure to the right).


Benchmarking data comparing high fidelity polymerases

High yield & specificity

Platinum SuperFi II DNA Polymerase amplifies a broad range of target lengths with high specificity and yield due to robustness of the enzyme and superior hot-start technology. The antibody-based Platinum hot-start technology inhibits enzyme activity until the initial PCR denaturation step, preventing nonspecific amplification and primer degradation while allowing greater yield of the target amplicons.

Composite of four gel images. Each gel has five lanes of samples

Figure 6. Versatility across broad range of amplicon lengths. Platinum SuperFi II DNA Polymerase (far left panel) provides high specificity and yield across range of DNA fragments from 0.3 kb to 14 kb amplified from 100 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases: A: Merck KOD™ Hot Start, B: KAPA HiFi HotStart PCR Kit, C: PrimeSTAR GXL. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

High sensitivity

The high sensitivity of Platinum SuperFi II DNA Polymerase enables detection of low-abundance DNA templates. High sensitivity is advantageous in experiments where there is a limited amount of starting material, or the target DNA is in low concentration in the sample.

Composite of four gel images; each gel has five lanes of samples

Figure 7. High sensitivity and reliable amplification from low amounts of input DNA. Platinum SuperFi II DNA Polymerase (far left panel) shows reliable amplification of a 2 kb fragment from 0.4 ng, 2 ng, 10 ng, 50 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane.). The same target was amplified with competitor DNA polymerases: A: PfuUltra™ II Fusion Hot Start, B: HotStar™ HiFidelity DNA Polymerase Kit, C: Expand HiFiPLUS Enzyme Blend. The estimated copy number is ~100 copies per 0.4 ng of human genomic DNA. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Inhibitor tolerance

Platinum SuperFi II DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased tolerance to common PCR inhibitors (Figure 8) such as, hemin, humic acid, and bile salt. 

Composite of five gel images; each gel has four lanes of samples

Figure 8. Platinum SuperFi II DNA Polymerase shows high tolerance to common PCR inhibitors. A 2 kb human genomic DNA fragment was amplified from 50 ng of human genomic DNA using Platinum SuperFi II DNA Polymerase or competitor high-fidelity DNA polymerases: A: Q5 Hot Start High-Fidelity, B: PrimeSTAR GXL, C: Merck KOD™ Hot Start, and D: KAPA HiFi HotStart PCR Kit in reaction mixtures containing 1: no inhibitor, 2: humic acid (4 µg/mL), 3: hemin (20 µM), and 4: bile salt (1 mg/mL). The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Benchtop stability

Extended stability of the Platinum SuperFi II DNA Polymerase enzyme at room temperature (Figure 9) enables high-throughput applications. Its superior Platinum hot-start technology enables benchtop stability and amplification with high specificity.

Gel image with 8 lanes

Figure 9. Assembled reactions with Platinum SuperFi II DNA Polymerase are stable room temperature. A 0.5 kb fragment was amplified form 50 ng of human genomic DNA. PCR reactions were set up and left at room temperature for 0 hr and 24 hr before loading to the Applied Biosystems ProFlex thermal cycler. Even after 24 hr of room-temperature setup, Platinum SuperFi II DNA Polymerase (lane P) produces results with high specificity and yields. The same experiment was also performed with competitor DNA polymerases: A: Q5 Hot Start High-Fidelity, B: KAPA HiFi HotStart PCR Kit, C: PrimeSTAR GXL. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

High yield & specificity

Platinum SuperFi II DNA Polymerase amplifies a broad range of target lengths with high specificity and yield due to robustness of the enzyme and superior hot-start technology. The antibody-based Platinum hot-start technology inhibits enzyme activity until the initial PCR denaturation step, preventing nonspecific amplification and primer degradation while allowing greater yield of the target amplicons.

Composite of four gel images. Each gel has five lanes of samples

Figure 6. Versatility across broad range of amplicon lengths. Platinum SuperFi II DNA Polymerase (far left panel) provides high specificity and yield across range of DNA fragments from 0.3 kb to 14 kb amplified from 100 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases: A: Merck KOD™ Hot Start, B: KAPA HiFi HotStart PCR Kit, C: PrimeSTAR GXL. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

High sensitivity

The high sensitivity of Platinum SuperFi II DNA Polymerase enables detection of low-abundance DNA templates. High sensitivity is advantageous in experiments where there is a limited amount of starting material, or the target DNA is in low concentration in the sample.

Composite of four gel images; each gel has five lanes of samples

Figure 7. High sensitivity and reliable amplification from low amounts of input DNA. Platinum SuperFi II DNA Polymerase (far left panel) shows reliable amplification of a 2 kb fragment from 0.4 ng, 2 ng, 10 ng, 50 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane.). The same target was amplified with competitor DNA polymerases: A: PfuUltra™ II Fusion Hot Start, B: HotStar™ HiFidelity DNA Polymerase Kit, C: Expand HiFiPLUS Enzyme Blend. The estimated copy number is ~100 copies per 0.4 ng of human genomic DNA. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Inhibitor tolerance

Platinum SuperFi II DNA Polymerase is engineered with a DNA-binding domain resulting in high processivity and increased tolerance to common PCR inhibitors (Figure 8) such as, hemin, humic acid, and bile salt. 

Composite of five gel images; each gel has four lanes of samples

Figure 8. Platinum SuperFi II DNA Polymerase shows high tolerance to common PCR inhibitors. A 2 kb human genomic DNA fragment was amplified from 50 ng of human genomic DNA using Platinum SuperFi II DNA Polymerase or competitor high-fidelity DNA polymerases: A: Q5 Hot Start High-Fidelity, B: PrimeSTAR GXL, C: Merck KOD™ Hot Start, and D: KAPA HiFi HotStart PCR Kit in reaction mixtures containing 1: no inhibitor, 2: humic acid (4 µg/mL), 3: hemin (20 µM), and 4: bile salt (1 mg/mL). The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Benchtop stability

Extended stability of the Platinum SuperFi II DNA Polymerase enzyme at room temperature (Figure 9) enables high-throughput applications. Its superior Platinum hot-start technology enables benchtop stability and amplification with high specificity.

Gel image with 8 lanes

Figure 9. Assembled reactions with Platinum SuperFi II DNA Polymerase are stable room temperature. A 0.5 kb fragment was amplified form 50 ng of human genomic DNA. PCR reactions were set up and left at room temperature for 0 hr and 24 hr before loading to the Applied Biosystems ProFlex thermal cycler. Even after 24 hr of room-temperature setup, Platinum SuperFi II DNA Polymerase (lane P) produces results with high specificity and yields. The same experiment was also performed with competitor DNA polymerases: A: Q5 Hot Start High-Fidelity, B: KAPA HiFi HotStart PCR Kit, C: PrimeSTAR GXL. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.


PCR de diferentes modelos com a polimerase de DNA Platinum SuperFi II

AT & GC-rich PCR

Platinum SuperFi II DNA Polymerase amplifies a broad range of sequences with high specificity due to robustness of the enzyme and its specially formulated buffer. The Platinum SuperFi II buffer enables amplification of GC-rich targets without a need for supplementary additives (Figure 10 and 11).

Gel image with 15 lanes

Figure 10. Robust amplification of AT-rich and GC-rich targets by Platinum SuperFi II DNA polymerase. Fifteen targets of varying GC content were amplified from 50 ng of human genomic DNA without any supplementary buffer additives that help with DNA denaturation. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Composite of four gel images; each gel has four lanes

Figure 11. Enhanced amplification of GC-rich targets. Platinum SuperFi II DNA Polymerase provides high specificity and yield of difficult GC-rich targets (far left panel) without any supplementary DNA-melting additives. Four GC-rich fragments (0.74 kb, 0.58 kb, 0.71 kb, and 0.72 kb in length; GC content indicated above) were amplified from 50 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases according to manufacturer recommended protocols for GC-rich PCR: A: PrimeSTAR GXL DNA Polymerase, B: KAPA HiFi HotStart PCR Kit in specialized reaction buffer for GC rich fragments, C: Merck KOD™ Hot Start DNA Polymerase with 10% DMSO added. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Long-range PCR

Due its high processivity and extremely low error rate, Platinum SuperFi II DNA Polymerase is ideal for accurately amplifying long fragments (up to 20 kb) with high yields and specificity (Figure 12). Amplification of longer targets (up to 40 kb) is possible but may require additional optimization such as using high-quality templates (pure, fresh, and intact) and fresh primer solutions (Figure 13).

Composite of two gels images, each gel has six lanes

Figure 12. Amplification of long fragments. Platinum SuperFi II DNA Polymerase (lane P) successfully amplifies 20 kb targets from 200 ng of human genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: A: Q5 Hot Start High-Fidelity, B: KAPA HiFi HotStart PCR Kit, C: Merck KOD Hot Start, D: PrimeSTAR GXL, and E: PfuUltra™ II Fusion HotStart. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Two gel images placed adjacent to each other, each gel has six lanes

Figure 13. Amplification of targets >20 kb. Platinum SuperFi II DNA Polymerase (lane P) successfully amplifies 30 kb targets and 40 kb targets from 50 ng of E. coli genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: A: Q5 Hot Start High-Fidelity, B: KAPA HiFi HotStart PCR Kit, C: Merck KOD™ Hot Start, D: PrimeSTAR GXL and E: PfuUltra II Fusion HotStart. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Multiplex PCR

Platinum SuperFi II DNA Polymerase can multiplex over a broad range of template concentrations in the buffer provided, without the need for significant optimization.

Gel image with four lanes
Figure 14. Multiplex PCR over a broad range of template concentrations using Platinum SuperFi II DNA Polymerase. 15 targets (99 bp; 131 bp; 160 bp; 199 bp; 251 bp; 300 bp; 345 bp; 400 bp; 516 bp; 613 bp; 735 bp; 908 bp; 1,005 bp; 1,190 bp; and 1,606 bp) were amplified from 2.5 ng, 25 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane). The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

FFPE DNA Amplification

Due to high sensitivity and inhibitor tolerance, Platinum SuperFi II DNA Polymerase enables efficient amplification of suboptimal quality DNA from formalin fixed paraffin embedded (FFPE) samples.

Gel image with 6 lanes of samples flanked by lanes of ladder

Figure 15. Amplification of FFPE-extracted DNA. Platinum SuperFi II DNA Polymerase successfully amplifies targets up to 0.4 kb from 10 ng of mouse FFPE DNA extracted using Invitrogen RecoverAll Total Nucleic Acid Isolation Kit for FFPE. The molecular weight marker is TrackIt 100 bp DNA Ladder.

AT & GC-rich PCR

Platinum SuperFi II DNA Polymerase amplifies a broad range of sequences with high specificity due to robustness of the enzyme and its specially formulated buffer. The Platinum SuperFi II buffer enables amplification of GC-rich targets without a need for supplementary additives (Figure 10 and 11).

Gel image with 15 lanes

Figure 10. Robust amplification of AT-rich and GC-rich targets by Platinum SuperFi II DNA polymerase. Fifteen targets of varying GC content were amplified from 50 ng of human genomic DNA without any supplementary buffer additives that help with DNA denaturation. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Composite of four gel images; each gel has four lanes

Figure 11. Enhanced amplification of GC-rich targets. Platinum SuperFi II DNA Polymerase provides high specificity and yield of difficult GC-rich targets (far left panel) without any supplementary DNA-melting additives. Four GC-rich fragments (0.74 kb, 0.58 kb, 0.71 kb, and 0.72 kb in length; GC content indicated above) were amplified from 50 ng of human genomic DNA. The same targets were also amplified using competitor DNA polymerases according to manufacturer recommended protocols for GC-rich PCR: A: PrimeSTAR GXL DNA Polymerase, B: KAPA HiFi HotStart PCR Kit in specialized reaction buffer for GC rich fragments, C: Merck KOD™ Hot Start DNA Polymerase with 10% DMSO added. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Long-range PCR

Due its high processivity and extremely low error rate, Platinum SuperFi II DNA Polymerase is ideal for accurately amplifying long fragments (up to 20 kb) with high yields and specificity (Figure 12). Amplification of longer targets (up to 40 kb) is possible but may require additional optimization such as using high-quality templates (pure, fresh, and intact) and fresh primer solutions (Figure 13).

Composite of two gels images, each gel has six lanes

Figure 12. Amplification of long fragments. Platinum SuperFi II DNA Polymerase (lane P) successfully amplifies 20 kb targets from 200 ng of human genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: A: Q5 Hot Start High-Fidelity, B: KAPA HiFi HotStart PCR Kit, C: Merck KOD Hot Start, D: PrimeSTAR GXL, and E: PfuUltra™ II Fusion HotStart. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Two gel images placed adjacent to each other, each gel has six lanes

Figure 13. Amplification of targets >20 kb. Platinum SuperFi II DNA Polymerase (lane P) successfully amplifies 30 kb targets and 40 kb targets from 50 ng of E. coli genomic DNA. Using the same primer sets, competitor DNA polymerases were also tested: A: Q5 Hot Start High-Fidelity, B: KAPA HiFi HotStart PCR Kit, C: Merck KOD™ Hot Start, D: PrimeSTAR GXL and E: PfuUltra II Fusion HotStart. The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

Multiplex PCR

Platinum SuperFi II DNA Polymerase can multiplex over a broad range of template concentrations in the buffer provided, without the need for significant optimization.

Gel image with four lanes
Figure 14. Multiplex PCR over a broad range of template concentrations using Platinum SuperFi II DNA Polymerase. 15 targets (99 bp; 131 bp; 160 bp; 199 bp; 251 bp; 300 bp; 345 bp; 400 bp; 516 bp; 613 bp; 735 bp; 908 bp; 1,005 bp; 1,190 bp; and 1,606 bp) were amplified from 2.5 ng, 25 ng, and 250 ng of human genomic DNA (template amounts are indicated above each lane). The molecular weight marker is TrackIt 1 Kb Plus DNA Ladder.

FFPE DNA Amplification

Due to high sensitivity and inhibitor tolerance, Platinum SuperFi II DNA Polymerase enables efficient amplification of suboptimal quality DNA from formalin fixed paraffin embedded (FFPE) samples.

Gel image with 6 lanes of samples flanked by lanes of ladder

Figure 15. Amplification of FFPE-extracted DNA. Platinum SuperFi II DNA Polymerase successfully amplifies targets up to 0.4 kb from 10 ng of mouse FFPE DNA extracted using Invitrogen RecoverAll Total Nucleic Acid Isolation Kit for FFPE. The molecular weight marker is TrackIt 100 bp DNA Ladder.


Diferenças entre a DNA polimerase Platinum SuperFi II e a polimerase de DNA Platinum SuperFi

Platinum SuperFi II DNA Polymerase comes with a reaction buffer that is specially formulated with iso-stabilizing components. This unique buffer composition offers several advantages: No Tm calculation for primers, more robust amplification of GC-rich target, enhanced amplification of long sequences, and a universal PCR protocol for high-throughput PCR (Table 1).

Table 1. Comparison of Platinum SuperFi II and Platinum SuperFi DNA Polymerases.

 

Product photo of package and three tubes of reagents

Platinum SuperFi II DNA Polymerase

Product photo of package and four tubes of reagents

Platinum SuperFi DNA Polymerase

Fidelity (vs. Taq)>300x>300x
Hot-start modificationYesYes
Tm calculator neededNo (primers anneal at 60°C)Yes
Universal PCR protocolYesNo
GC-rich amplificationYes (GC enhancer not required)Yes (GC enhancer recommended)
Long-range amplificationUp to 20 kb (enhanced performance)Up to 20 kb
Benchtop stabilityUp to 24 hrUp to 24 hr
Inhibitor toleranceHighHigh
3′ end of ampliconsBluntBlunt
Certified low level of residual DNA (per 50 μL rxn)≤1 copy of bacterial DNA
≤0.3 copy of human DNA  
≤1 copy of bacterial DNA
≤0.2 copy of human DNA
Formats

Stand-alone polymerase:
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Green master mix:
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Stand-alone polymerase:
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 Benchmarking data of Platinum SuperFi II and Platinum SuperFi DNA polymerases



References

UsageReference
Amplification of SARS-CoV-2 whole genome for Illumina sequencingBotelho-Souza LF, Nogueira-Lima, FS, Roca TP et al. (2021) SARS-CoV-2 genomic surveillance in Rondônia, Brazilian Western Amazon. Sci Rep 11: 3770. 
Target capture and enrichment of SARS-CoV-2 cDNA pools for Illumina sequencingChoudhury Y, Cher CY, Wan ZY et al. (2021) A viral fragmentation signature for SARS-CoV-2 in clinical samples correlating with contagiousness. medRxiv doi: https://doi.org/10.1101/2021.01.11.21249265 
Cloning of SARS-CoV-2 whole genome for functional studiesKotaki T, Xie X, Shi PY et al. (2021) A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation. Sci Rep 11: 2229. 
RT-PCR of viral genes from human serum samples, followed by Sanger sequencingLan Y, He X, Xin R (2021) Genetic characteristics of HIV-1 CRF12_BF first identified in Guangdong province, China. AIDS Res Hum Retroviruses 37(2):157–161. 
Amplification of HIV-1 geneLugongolo MY, Manoto SL, Maphanga C et al. (2021) Label-free detection of mutations in the HIV genome using a surface plasmon resonance biosensor. SPIE BiOS https://doi.org/10.1117/12.2578317
Amplification of SARS-CoV-2 whole genome for Illumina sequencingNascimento VAD, Corado ALG, Nascimento FOD et al. (2020) Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil. Mem Inst Oswaldo Cruz 115:e200310.
Cloning of SARS-CoV1 and SARS-CoV-2 spike variants for binding assaysStamatatos L, Czartoski J, Wan YH et al. (2021) Antibodies elicited by SARS-CoV-2 infection and boosted by vaccination neutralize an emerging variant and SARS-CoV-1. medRxiv doi: https://doi.org/10.1101/2021.02.05.21251182
Cloning of large viral gene fragments for whole genome assemblyXie X, Lokugamage KG, Zhang X et al. (2021) Engineering SARS-CoV-2 using a reverse genetic system. Nat Protoc 16(3):1761–1784.
Cloning of SARS-CoV-2 whole genome for functional studiesXie X, Muruato A, Lokugamage KG, et al. (2020) An Infectious cDNA Clone of SARS-CoV-2. Cell Host Microbe 27(5):841–848.
UsageReference
Amplification of SARS-CoV-2 whole genome for Illumina sequencingBotelho-Souza LF, Nogueira-Lima, FS, Roca TP et al. (2021) SARS-CoV-2 genomic surveillance in Rondônia, Brazilian Western Amazon. Sci Rep 11: 3770. 
Target capture and enrichment of SARS-CoV-2 cDNA pools for Illumina sequencingChoudhury Y, Cher CY, Wan ZY et al. (2021) A viral fragmentation signature for SARS-CoV-2 in clinical samples correlating with contagiousness. medRxiv doi: https://doi.org/10.1101/2021.01.11.21249265 
Cloning of SARS-CoV-2 whole genome for functional studiesKotaki T, Xie X, Shi PY et al. (2021) A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation. Sci Rep 11: 2229. 
RT-PCR of viral genes from human serum samples, followed by Sanger sequencingLan Y, He X, Xin R (2021) Genetic characteristics of HIV-1 CRF12_BF first identified in Guangdong province, China. AIDS Res Hum Retroviruses 37(2):157–161. 
Amplification of HIV-1 geneLugongolo MY, Manoto SL, Maphanga C et al. (2021) Label-free detection of mutations in the HIV genome using a surface plasmon resonance biosensor. SPIE BiOS https://doi.org/10.1117/12.2578317
Amplification of SARS-CoV-2 whole genome for Illumina sequencingNascimento VAD, Corado ALG, Nascimento FOD et al. (2020) Genomic and phylogenetic characterisation of an imported case of SARS-CoV-2 in Amazonas State, Brazil. Mem Inst Oswaldo Cruz 115:e200310.
Cloning of SARS-CoV1 and SARS-CoV-2 spike variants for binding assaysStamatatos L, Czartoski J, Wan YH et al. (2021) Antibodies elicited by SARS-CoV-2 infection and boosted by vaccination neutralize an emerging variant and SARS-CoV-1. medRxiv doi: https://doi.org/10.1101/2021.02.05.21251182
Cloning of large viral gene fragments for whole genome assemblyXie X, Lokugamage KG, Zhang X et al. (2021) Engineering SARS-CoV-2 using a reverse genetic system. Nat Protoc 16(3):1761–1784.
Cloning of SARS-CoV-2 whole genome for functional studiesXie X, Muruato A, Lokugamage KG, et al. (2020) An Infectious cDNA Clone of SARS-CoV-2. Cell Host Microbe 27(5):841–848.
Polimerase de DNA Platinum Super Fi

A Invitrogen Platinum SuperFi DNA é uma polimerase de DNA com atividade revisora que combina a fidelidade superior com a confiável tecnologia hot-start Platinum, desenvolvida para fornecer o maior sucesso em PCR. Com a fidelidade >100x Taq, a polimerase de DNA Platinum SuperFi é ideal para clonagem, mutagenese e outras aplicações que se beneficiam da precisão suprema da sequência gerada.

  • Excepcional fidelidade >100x Taq
  • Alta especificidade e maior rendimento com a tecnologia hot-start Platinum
  • Amplificação robusta de alvos difíceis de amplificar, incluindo aqueles de pureza não ideal ou com conteúdo de GC ˃65%
  • Fluxo de trabalho prático com a montagem da reação à temperatura ambiente e carregamento direto no gel com o uso dos tampões verdes

 Calculadora de T m

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