Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan probes. TaqMan MicroRNA Assays are:

  • Highly specific—quantitate only mature miRNAs, not precursors
  • Sensitive—conserve limited samples; requires only 1-10 nanograms of total RNA or equivalent
  • Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours

Our TaqMan MicroRNA Assays catalog is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.

Assay search tool - find & buy your single tube TaqMan Assays:

Availability & sizes: Made to Order assays are available in four different scales and usually ship within two weeks. Inventoried assays are prepackaged in Small scale and are ready to ship. Inventoried assays may also be purchased in larger sizes by ordering as MTO.


TaqMan miRNA Assay selection guide

Format

Recommended TaqMan assay/array

Individual qPCR assays

TaqMan miRNA Assays
Comprehensive collection of pre-designed TaqMan qPCR assays for human, mouse, rat, and nearly 100 other species.

2,000 pre-designed assays are inventoried and ship out in 1-2 days.

Search for pre-designed assays ›

384-well microfluidic TaqMan array card

TaqMan miRNA array cards
Small reaction volumes and streamlined workflow enable profiling up to 380 miRNA targets from as little as 1 ng of total RNA.

Search for pre-designed 384-well arrays ›
Design your own custom 384-well array ›

OpenArray plate

Predesigned TaqMan miRNA OpenArray panels
Run three samples per QuantStudio 12K Flex OpenArray plate, targeting 750 mature miRNAs per sample.

TaqMan Human miRNA OpenArray panel ›
TaqMan Rodent miRNA OpenArray panel ›

For custom miRNA OpenArray panels, contact: QuantStudioFrontDesk@thermofisher.com

Custom 96- and 384-well plates

Contact: specialty_plates@thermofisher.com

SpeciesTaqMan® MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.
Assay ComponentsTaqMan® MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan® Assay (TaqMan® probe and PCR primer set).
Available AssaysDownload: Complete list of TaqMan® MicroRNA Assays
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

Catalog #Availability# of RT/PCR Reactions
4427975InventoriedS: 50/150
4440885Made to OrderXS: 25/75
4440886Made to OrderS: 50/150
4440887Made to OrderM: 750/750
4440888Made to OrderL: 2900/2900
Related products, categories & applications

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Related Applications

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Specifications
SpeciesTaqMan® MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.
Assay ComponentsTaqMan® MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan® Assay (TaqMan® probe and PCR primer set).
Available AssaysDownload: Complete list of TaqMan® MicroRNA Assays
Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

View all sizes
Catalog #Availability# of RT/PCR Reactions
4427975InventoriedS: 50/150
4440885Made to OrderXS: 25/75
4440886Made to OrderS: 50/150
4440887Made to OrderM: 750/750
4440888Made to OrderL: 2900/2900
Documentation

TaqMan Assay documentation files can be downloaded at thermofisher.com/taqmanfiles

Product Literature

Application Note: Endogenous Controls for Real-Time Quantitation of miRNA Using TaqMan® MicroRNA Assays
Application Note: High Correlation of miRNA Quantitation Data from Matched FFPE and Snap-Frozen Tissues using TaqMan® MicroRNA Assays
Customer Focus Story: TaqMan® MicroRNA Assays help researchers validate miRNA markers for forensic body fluid identification
Poster: microRNA Quantitation by Real-time PCR
Poster: TaqMan-based miRNA Profiles Classify Embryonic Stem and Differentiated Cells
Product Bulletin: Quantitate microRNAs with the specificity and sensitivity of real-time PCR
QRC: TaqMan® Small RNA Assays
Design Process for Small RNA Assays
TaqMan® MicroRNA Assays FAQs
Brochure: TaqMan microRNA Assays

Resources
Support

Description

TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. TaqMan® MicroRNA Assays are:

  • Highly specific—quantitate only mature miRNAs, not precursors
  • Sensitive—conserve limited samples; requires only 1-10 nanograms of total RNA or equivalent
  • Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours

This product can be purchased by visiting AppliedBiosystems.com.
Direct ordering through the Life Technologies website will be available soon.

Specifications

General Specifications

Species:TaqMan® MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry
Assay Components:TaqMan® MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan® Assay (TaqMan® probe and PCR primer set).
Available Assays:Download: Complete list of TaqMan® MicroRNA Assays

Shipping and Storage

Shipped at ambient temperature.
Store at –15ºC to –25ºC.

White Paper: Ambient Temperature Shipping Reduces Environmental Impact and Retains Quality and Stability

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