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Use the latest advance in protein digestion for peptide mapping and quantitation applications. Thermo Scientific SMART Digest Kits make protein digestion:
Protein digestion kits use optimized heat-stable enzymes on magnetic or non-magnetic beads. Immunoaffinity (IA) kits couple enzymatic digestion with IA capture on a single bead for high sensitivity. This innovation generates high confidence in your HPLC and LC/MS results, and it can be automated. SMART Digest Kits especially benefit biomarker and biopharmaceutical characterization and quantitation applications.
Choose between optimized heat-stable trypsin, chymotrypsin, or proteinase K enzymatic digestion for sequence-specific cleavage of your protein. The enzyme, on magnetic or non-magnetic beads, is activated at elevated temperatures for accelerated digestion under protein denaturing conditions.
Catalog | Description | Each Kit Includes | |
---|---|---|---|
Order now | 60109-101 | SMART DIGEST KIT Trypsin | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Order now | 60109-104 | SMART DIGEST KIT Chymotrypsin | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Order now | 60109-107 | SMART DIGEST KIT Proteinase K | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Order now | 60109-110 | SMART DIGEST KIT Pepsin | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Order now | 60109-102 | SMART DIGEST KIT Trypsin | 96 PCR tubes containing SMART Digest Resin Collection Plate 96 Well Filter plate |
Order now | 60109-105 | SMART DIGEST KIT Chymotrypsin | 96 PCR tubes containing SMART Digest Resin Collection Plate 96 Well Filter plate |
Order now | 60109-108 | SMART DIGEST KIT Proteinase K | 96 PCR tubes containing SMART Digest Resin Collection Plate 96 Well Filter plate |
Order now | 60109-111 | SMART DIGEST KIT Pepsin | 96 PCR tubes containing SMART Digest Resin Collection Plate 96 Well Filter plate |
Order now | 60109-103 | SMART DIGEST KIT Trypsin | 96 PCR tubes containing SMART Digest Resin Collection Plate SOLAµ 96 Well SPE microelution plate |
Order now | 60109-106 | SMART DIGEST KIT Chymotrypsin | 96 PCR tubes containing SMART Digest Resin Collection Plate SOLAµ 96 Well SPE microelution plate |
Order now | 60109-109 | SMART DIGEST KIT Proteinase K | 96 PCR tubes containing SMART Digest Resin Collection Plate SOLAµ 96 Well SPE microelution plate |
Order now | 60109-112 | SMART DIGEST KIT Pepsin | 96 PCR tubes containing SMART Digest Resin Collection Plate SOLAµ 96 Well SPE microelution plate |
Catalogue | Description | Each kit Includes | |
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Order now | 60109-101-MB | SMART Digest Kit Trypsin |
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Order now | 60109-104-MB | SMART Digest Kit Chymotrypsin | |
Order now | 60109-107-MB | SMART Digest Kit Proteinase K | |
Order now | 60109-110-MB | SMART Digest Kit Pepsin | |
Order now | 60109-102-MB | SMART Digest Kit Trypsin |
|
Order now | 60109-105-MB | SMART Digest Kit Chymotrypsin | |
Order now | 60109-108-MB | SMART Digest Kit Proteinase K | |
Order now | 60109-103-MB | SMART Digest Kit Trypsin |
|
Order now | 60109-106-MB | SMART Digest Kit Chymotrypsin | |
Order now | 60109-109-MB | SMART Digest Kit Proteinase K |
Catalogue | Description | Kits Include | |
---|---|---|---|
Order now | 60109-101-B | SMART Digest Kit Trypsin |
|
Order now | 60109-104-B | SMART Digest Kit Chymotrypsin | |
Order now | 60109-107-B | SMART Digest Kit Proteinase K | |
Order now | 60109-110-B | SMART Digest Kit Pepsin | |
Order now | 60109-102-B | SMART Digest Kit Trypsin |
|
Order now | 60109-105-B | SMART Digest Kit Chymotrypsin | |
Order now | 60109-108-B | SMART Digest Kit Proteinase K | |
Order now | 60109-103-B | SMART Digest Kit Trypsin |
|
Order now | 60109-106-B | SMART Digest Kit Chymotrypsin | |
Order now | 60109-109-B | SMART Digest Kit Proteinase K |
Catalogue | Description | |
---|---|---|
Order now | 60114-101 | SMART Digest Bulk Fractionation Kit, Protein A Non-Magnetic & Soluble Smart |
Order now | 60115-101 | SMART Digest Bulk Fractionation Kit, Protein G Non-Magnetic & Soluble Smart |
Order now | 60114-102 | SMART Digest Bulk Fractionation Kit, Protein A Non-Magnetic with SOLAµ/Collection plate & Soluble Smart |
Order now | 60115-102 | SMART Digest Bulk Fractionation Kit, Protein G Non Magnetic with SOLAµ/Collection plate & Soluble Smart |
Order now | 60114-103 | SMART Digest Bulk Fractionation Kit, Protein A Magnetic with SOLAµ/Collection plate & SOLUBLE SMART |
Order now | 60115-103 | SMART Digest Bulk Fractionation Kit, Protein G Magnetic with SOLAµ/Collection plate & Soluble smart |
Order now | 60114-104 | SMART Digest Bulk Fractionation Kit, Protein A Magnetic & Soluble Smart |
Order now | 60115-104 | SMART Digest Bulk Fractionation Kit, Protein G Magnetic & Soluble Smart |
Order now | 60116-101 | SMART Digest Bulk Fractionation Kit, Protein A Magnetic standalone |
Order now | 60117-101 | SMART Digest Bulk Fractionation Kit, Protein G Magnetic standalone |
Order now | 60118-101 | SMART Digest Bulk Fractionation Kit Streptavidin Magnetic without Soluble Trypsin |
Order now | 60119-103 | SMART Digest Bulk Fractionation Kit Streptavidin Magnetic with Soluble Trypsin, Collection plate and SOLAu |
Order now | 60119-104 | SMART Digest Bulk Fractionation Kit Streptavidin Magnetic with Soluble Trypsin |
Catalogue | Description | Each Kit Includes | |
---|---|---|---|
Order now | 60109-101-LPH | SMART DIGEST low pH KIT Trypsin | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Catalogue | Description | Each Kit Includes | |
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Order now | 60109-101-MB-LPH | SMART DIGEST low pH KIT Trypsin Magnetic beads | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Order now | 60109-104-MB-LPH | SMART DIGEST KIT CHYMOTRYPSIN Magnetic bulk low pH buffer | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Catalogue | Description | Each Kit Includes | |
---|---|---|---|
Order now | 60109-101-MB-LPH | SMART DIGEST low pH KIT Trypsin Magnetic beads | 96 PCR tubes containing SMART Digest Resin Collection Plate |
Order now | 60109-104-MB-LPH | SMART DIGEST KIT CHYMOTRYPSIN Magnetic bulk low pH buffer | 96 PCR tubes containing SMART Digest Resin Collection Plate |
SMART Digest IA Kits perform trypsin digestion with immunoaffinity capture in a single well. The immunoaffinity moiety (streptavidin, protein A, or protein G) is typically employed to increase sensitivity by purifying your low-level proteins from complex biological matrices.
Catalogue | Description | |
---|---|---|
Order now | 60110-101 | SMART Digest IA Kit, Streptavidin non-magnetic |
Order now | 60110-102 | SMART Digest IA Kit, Streptavidin non-magnetic with Thermo Scientific SOLAµ SPE and collection plate |
Order now | 60110-103 | SMART Digest IA Kit, Streptavidin magnetic with SOLAµ SPE and collection plate |
Order now | 60110-104 | SMART Digest IA Kit, Streptavidin magnetic |
Catalogue | Description | |
---|---|---|
Order now | 60111-101 | SMART Digest IA Kit, Protein A non-magnetic |
Order now | 60111-102 | SMART Digest IA Kit, Protein A non-magnetic with SOLAµ SPE and collection plate |
Order now | 60111-103 | SMART Digest IA Kit, Protein A magnetic with SOLAµ SPE and collection plate |
Order now | 60111-104 | SMART Digest IA Kit, Protein A magnetic |
Catalogue | Description | |
---|---|---|
Order now | 60112-101 | SMART Digest IA Kit, Protein G non-magnetic |
Order now | 60112-102 | SMART Digest IA Kit, Protein G non-magnetic with SOLAµ SPE and collection plate |
Order now | 60112-103 | SMART Digest IA Kit, Protein G magnetic with SOLAµ SPE and collection plate |
Order now | 60112-104 | SMART Digest IA Kit, Protein G magnetic |
Obtain high-quality analytical results from mRNA digestions using SMART Digest RNase Kits. A significant advance in sample preparation for oligonucleotide mapping and quantitation, these kits provide fast and simple mRNA digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that is compatible with automation.
Typical SMART Digest Digestion Times | ||||
---|---|---|---|---|
Protein | Digestion Time | Protein | Digestion Time | |
Insulin | 4 min | IgG in 50 µL plasma* | 75 min | |
BSA | < 5 min | Ribonuclease A | 150 min | |
Carbonic anhydrase | < 5 min | Thyroglobulin | 240 min | |
Lysozyme | < 5 min | C-reactive protein | 240 min | |
Apo-B 30 IgG | 45 min |
200μL protein solution (100 μg/mL) at 70 °C
* IgG in plasma (17.5mg/mL total protein) at 70 °C
Peptide mapping and quantitation are fundamental workflows used in the development of biotherapeutics to ensure structural integrity, drug efficacy and patient safety. Current sample preparation techniques are complex, laborious, prone to error, and not amenable to automation impacting reanalysis rates, transfer, and implementation of methods at different sites.
SMART Digest protein digestion provides a simple process on magnetic bead technology which allows it to be automated on platforms like the Thermo Scientific King Fisher Duo to enable easy, fast, and highly reproducible results.
Dr. Ken Cook (Thermo Fisher Scientific) and Dr. Sara Carillo (National Institute for Bioprocessing Research and Training -NIBRT) discuss:
Learn about four new technologies that dramatically improve peptide mapping workflows and increases confidence.
Dr. Ken Cook (Thermo Fisher Scientific) and Dr. Sara Carillo (National Institute for Bioprocessing Research and Training -NIBRT) discuss:
Watch this introductory animation to learn how SMART Digest kits provide easy, fast, and highly reproducible protein digestion.
Save time and get better results with the SMART Digest Kit and automate your digestion with a KingFisher system with a touch of a button.
Access the latest interactive version of our Chromatography Consumables & Columns Catalog. Our enhanced online version features easier access to product information, key application notes and brand-new selector tools.
Find interactive tools and calculators, guides to select the right products, equivalent product finders, and get in contact with our consumables technical support team to answer your application questions.
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