A variety of nunc cultureware multiwell plates

Cell line data table of contents

Nunclon Supra product photo

NEW Nunclon Supra Surfaces for advanced cell culturing

We are proud to provide a new product line surface treatment that will support improved cell yield and morphology as well enabling you to do xeno-free or low-serum applications.

Order yours today:  Dishes   |  Flasks  |  Plates  |  96-well plates

See how our surfaces perform with 39 cell types and cell lines at thermofisher.com/supradata

For Nunclon Delta surfaces:

For Nunclon Sphera surfaces:

Nunclon surface performance data

We’ve tested the most-used cell lines on Thermo Scientific Nunclon Delta, Supra and Sphera cell culture plastics side-by-side with the leading competitor to validate Nunc cell culture plastics’ place in your cell culture hood.

Please note: We are adding data for more cells all the time.

A431 doubling times and gene expression

A431 and Nunclon Delta surfaces

A431 cells grown on Nunc Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

A431 morphology with Nunclon Delta surfaces

Below are representative brightfield images of A431 cells grown on Nunclon Delta surfaces (left) and Corning® tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of A431 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

A431 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of A431 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of A431 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing A431 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

A431 gene expression with Nunclon Delta surfaces

The below image describes A431 cells grown on Nunclon Delta plates or Corning® TC surfaces for at least 10 passage doublings were assayed for cell health with respect to expression of two common endogenous genes, EGFR (epidermal growth factor receptor) and VEFGA (vascular endothelial growth factor alpha). The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is shown on the y-axis.

Bar charts showing gene expression of 2 endogenous genes in A431 cells when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Please note: For the above A431 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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A549 doubling times and EGFR activation

A549 and Nunclon Delta surfaces

A549 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

A549 morphology with Nunclon Delta surfaces

Below are representative brightfield images of A549 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification. 

brightfield images of A549 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

A549 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of A549 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of A549 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing A549 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

A549 EGFR activation with Nunclon Delta surfaces

A549 cells grown on Nunclon Delta surfaces (Nunc) or Corning TC (Corning) surfaces as well as those switched to Nunclon Delta surfaces (S to ND) surface, were treated with epidermal growth factor (EGF, 200 ng/mL) for 10 minutes. Total cell lysates (30 mg) were subjected to western blot analysis for EGFR phosphorylation using anti-phospho-EGFR (Tyr1086) rabbit polyclonal antibody 0.5 µg/mL. Total EGFR and GAPDH were used as experimental and normalization controls respectively.

Western blot image showing location of phospho-EGFR protein when grown on Nunclon Delta or on Corning TC surface and Bar chart showing fold change in phospho-EGFR expression in A549 cells when grown on Nunclon Delta or on Corning TC surface

Please note: For the above A549 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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Caco-2 doubling times, morphology, and cell attachment

Caco-2 and Nunclon Delta surfaces

Caco-2 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

Caco-2 morphology with Nunclon Delta surfaces

Below are representative brightfield images of Caco-2 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification. 

brightfield images of Caco-2 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

Caco-2 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of Caco-2 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of Caco-2 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing Caco-2 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Caco-2 cell attachment with Nunclon Delta surfaces

As an extension of cell attachment and growth, Caco-2 cells grown on Nunclon Delta surfaces (left) and Corning® TC surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface (right) were stained for the tight junction protein ZO-3, shown in green. The nucleus was stained with DAPI (shown in blue). No morphological difference was observed between the surfaces. Cells were grown on 6-well plates and stained on the same surface. Images were captured using EVOS M5000 imaging system under 20X magnification and cropped using the same aspect ratio for better visualization.

fluorescence microscopy images of Caco-2 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

Please note: For the above Caco-2 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 20% Gibco FBS, and 0.5% Penicillin Streptomycin.

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CHO-K1 doubling times, morphology, and transfection efficiency

CHO-K1 and Nunclon Delta surfaces

CHO-K1 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

CHO-K1 morphology with Nunclon Delta surfaces

Below are representative brightfield images of CHO-K1 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of CHO-K1 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

CHO-K1 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of CHO-K1 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of CHO-K1 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing CHO-K1 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

CHO-K1 transfection efficiency with Nunclon Delta surfaces

CHO-K1 cells seeded on Nunclon Delta surfaces and Corning TC surface (6-well plates) and transfected with mCherry using Neon Transfection System. Representative images of live cells were acquired 24 hours post transfection.

Please note: Flow cytometry evaluation of transfection efficiency of mCherry in CHO-K1 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.

Brightfield and fluorescence images showing CHO-K1 cells and mCherry fluorescent protein localization
Brightfield and fluorescence images showing CHO-K1 cells and mCherry fluorescent protein localization
flow cytometry histograms of fluorescently stained CHO-K1 cells grown on Nunclon Delta surface or on Corning TC surface

Please note: For the above CHO-K1 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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Cos-7 doubling times and morphology

Cos-7 and Nunclon Delta surfaces

Cos-7 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

Cos-7 morphology with Nunclon Delta surfaces

Below are representative brightfield images of Cos-7 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification. 

rightfield images of Cos-7 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

Cos-7 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of Cos-7 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis
  2. Graph (B) shows mean population doubling time of Cos-7 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing Cos-7 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Please note: For the above Cos-7 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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HEK293 doubling times, morphology, and transfection efficiency

HEK293 and Nunclon Delta surfaces

HEK293 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

HEK293 morphology with Nunclon Delta surfaces

Below are representative brightfield images of HEK293 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of HEK293 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

HEK293 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of HEK293 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of HEK293 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
bar charts showing HEK293 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

HEK293 transfection efficiency with Nunclon Delta surfaces

HEK293 cells seeded on Nunclon Delta surfaces or Corning TC surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.

Please note: Flow cytometry evaluation of transfection efficiency of mCherry in HEK293 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.

fluorescence images of HEK293 cells grown on Nunclon Delta or on Corning TC surface
flow cytometry histograms of fluorescently stained HEK293 cells grown on Nunclon Delta surface or on Corning TC surface

Please note: For the above HEK293 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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HeLa doubling times, morphology, and transfection efficiency

HeLa and Nunclon Delta surfaces

HeLa cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

HeLa morphology with Nunclon Delta surfaces

Below are representative brightfield images of HeLa cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of HeLa cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

HeLa cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of HeLa cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of HeLa cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing HeLa cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

HeLa transfection efficiency with Nunclon Delta surfaces

HeLa cells seeded on Nunclon Delta surfaces or Corning TC surfaces (6-well plates) and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.

Please note: Flow cytometry evaluation of transfection efficiency of mCherry in HeLa cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.

fluorescence images of HeLa cells grown on Nunclon Delta or on Corning TC surface
flow cytometry histograms of fluorescently stained HeLa cells grown on Nunclon Delta surface or on Corning TC surface

Please note: For the above HeLa Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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HepG2 doubling times, morphology, and gene expression

HepG2 and Nunclon Delta surfaces

HepG2 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

HepG2 morphology with Nunclon Delta surfaces

Below are representative brightfield images of HepG2 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of HepG2 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

HepG2 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of HepG2 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of HepG2 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing HepG2 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

HepG2 gene expression with Nunclon Delta surfaces

HepG2 cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for cell health with respect to expression of two common endogenous genes, SLCO2B1 (solute carrier organic anion transporter family member 2B1) and ABCC6 (multidrug resistance associated protein 6). The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is shown on the y-axis.

Bar charts showing gene expression of 2 endogenous genes in HepG2 cells when grown on Nunclon Delta or on Corning TC surface
Bar charts showing HepG2 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Please note: For the above HepG2 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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HT-29 doubling times, morphology, and protein expression

HT-29 cells and Nunclon Delta surfaces

HT-29 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

HT-29 morphology with Nunclon Delta surfaces

Below are representative brightfield images of HT-29 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of HT-29 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

HT-29 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of HT-29 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of HT-29 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing HT-29 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

HT-29 protein expression with Nunclon Delta surfaces

  1. HT-29 cells grown on Nunclon Delta surfaces (left panel) and Corning TC surfaces (right panel) 6-well plates were fixed on the plate and stained for the junction protein ZO-3 using ABfinity Anti-ZO-3 Recombinant Rabbit Oligoclonal Antibody (5 mg/mL) and detected using goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (1:2,000). Panel a shows representative cells that were stained for detection and localization of ZO-3 protein (green), Panel b represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin (1:300). Panel c is stained for nuclei (blue) using Hoechst 33342 (1:2,000). Panel d is a composite image of Panels a, b and c demonstrating membrane localization of ZO-3. Scale bar=100 μm.
  2. As an extension of cell growth and confluency, ZO-3 expression was further detected by western blot at days 2 and 6 of HT-29 cells grown on Nunclon Delta surfaces (Nunc) or Corning TC surfaces (Corning) within 100 mm dishes, as well as those switched to Nunclon Delta surfaces surface (S to ND). The blot was probed with 1 µg/mL of ZO-3 antibody. A 140 kDa band corresponding to ZO-3 was observed across all lanes. Additionally, a 100 kDa band corresponding to ZO-3 isoform was specifically expressed in confluent cells (6 days).
Fluorescence microscopy images of HT-29 cells stained for ZO-3 and F-actin when grown on Nunclon Delta or on Corning TC surface and Western blot image showing location of ZO-3 protein in HT-29 cells when grown on Nunclon Delta or on Corning TC surface

Please note: For the above HT-29 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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MCF-7 doubling times, morphology, and surface marker expression

MCF-7 cells and Nunclon Delta surfaces

MCF-7 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

MCF-7 morphology with Nunclon Delta surfaces

Below are representative brightfield images of MCF-7 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of MCF-7 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

MCF-7 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of MCF-7 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of MCF-7 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing MCF-7 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

MCF-7 surface marker expression with Nunclon Delta surfaces

MCF-7 cells grown on Nunclon Delta surfaces (left) or Corning TC surfaces (middle) as well as those switched to Nunclon Delta surfaces surface (right) were analyzed for the expression of endogenous receptor tyrosine protein kinase ErbB2 using flow cytometry. IgG1 was used as the isotype control. No difference was observed in the relative expression of ErbB2 using the different growth surfaces. Samples were run in duplicate on an Invitrogen Attune NxT Flow Cytometer.

Please note: For the above MCF-7 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco MEM, 10% Gibco FBS, 1% Penicillin Streptomycin, and 0.01 mg/ml Insulin.

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MCF-10A doubling times, morphology, and gene expression

MCF-10A cells and Nunclon Delta surfaces

MCF-10A cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

MCF-10A morphology with Nunclon Delta surfaces

Below are representative brightfield images of MCF-10A cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification

brightfield images of MCF-10A cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

MCF-10A cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of MCF-10A cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of MCF-10A cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing MCF-10A cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

MCF-10A gene expression with Nunclon Delta surfaces

MCF-10A cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for the expression of cell surface associated MUC1 (mucin-1) gene by qPCR. RNA was isolated using RiboPure RNA Purification Kit, cDNA was prepared using High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor, and qPCR was performed using TaqMan Fast Advanced Master Mix in a Custom TaqMan Array Plate. The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is plotted on the y-axis.

Bar chart showing gene expression of 2 endogenous genes in MCF-10A cells when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Please note: For the above MCF-10A Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Reconstituted Gibco Medium 171, 100 ng/ml cholera toxin, and 1% Penicillin Streptomycin.

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MDA-MB-231 doubling times, morphology, and surface marker expression

MDA-MB-231 cells and Nunclon Delta surfaces

MDA-MB-231 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

MDA-MB-231 morphology with Nunclon Delta surfaces

Below are representative brightfield images of MDA-MB-231 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of MDA-MB-231 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

MDA-MB-231 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of MDA-MB-231 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis
  2. Graph (B) shows mean population doubling time of MDA-MB-231 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing MDA-MB-231 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

MDA-MB-231 surface marker expression with Nunclon Delta surfaces

MDA-MB-231 cells grown on Nunclon Delta surfaces or Corning TC surface for 25 doublings as well as those switched to Nunclon Delta surfaces (ND) surface for 10 doublings were assessed for the expression of two endogenous surface marker proteins: ICAM-1 (top row, 0.25 μg/test) and CD44 (bottom row, 0.06 μg/test) using flow cytometry. 10,000 events were collected for each condition. No difference was observed in the surface marker expression between different growth surfaces.

Flow cytometry histograms showing magnitude of ICAM-1 and CD44 signal in MDA-MB-231 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

Please note: For the above MDA-MB-231 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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MDCK doubling times, morphology, and gene expression

MDCK cells and Nunclon Delta surfaces

MDCK cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

MDCK morphology with Nunclon Delta surfaces

Below are representative brightfield images of MDCK cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of MDCK cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

MDCK cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of MDCK cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of MDCK cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing MDCK cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

MDCK gene expression with Nunclon Delta surfaces

As an extension to cell attachment, MDCK cells grown on Nunclon Delta surfaces or Corning TC surfaces for at least 10 passage doublings were assayed for the gene expression of endogenously expressed CDH-1 (cadherin-1), a cell adhesion marker. Cells were also assayed for expression of the characteristic endogenous gene MUC1 (mucin-1) by qPCR. RNA was isolated using RiboPure RNA purification kit, cDNA was prepared using a cDNA preparation kit, and qPCR was performed using TaqMan Assay Master Mix in a custom TaqMan array plate. The normalized relative gene expression of cells grown on Corning TC surface with respect to Nunclon Delta surfaces surface is plotted on the y-axis.

Bar charts showing fold change in gene expression for CDH-1 and MUC1 in MDCK cells when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Please note: For the above MDCK Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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NIH3T3 doubling times, morphology, and wound healing

NIH3T3 cells and Nunclon Delta surfaces

NIH3T3 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

NIH3T3 morphology with Nunclon Delta surfaces

Below are representative brightfield images of NIH3T3 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of NIH3T3 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

NIH3T3 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of NIH3T3 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of NIH3T3 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis
Bar charts showing NIH3T3 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

NIH3T3 wound healing with Nunclon Delta surfaces

  1. NIH3T3 cells grown on Nunclon Delta surfaces or Corning TC surfaces were assessed for cell health by their ability to undergo wound healing under serum-starved conditions on the respective surfaces.
  2. Quantification of images shown in A. Images were analyzed using ImageJ. The percent of wounded area over time is plotted on the y-axis. Error bars represent SEM. Data are collected from 3 separate fields for each condition. Images were captured using EVOS Cell Imaging Systems under 10X magnification.
Microscopic images and chart showing NIH3T3 wound healing data and data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Please note: For the above NIH3T3 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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PC-3 doubling times, morphology, and transfection efficiency

PC-3 cells and Nunclon Delta surfaces

PC-3 cells grown on Nunclon Delta surfaces, in combination with Gibco cell culture media and fetal bovine serum have been tested to support consistent cell growth.

PC-3 morphology with Nunclon Delta surfaces

Below are representative brightfield images of PC-3 cells grown on Nunclon Delta surfaces (left) and Corning tissue culture (TC)-treated surfaces (middle), and those switched to Nunclon Delta surfaces (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

brightfield images of PC-3 cells grown on Nunclon Delta surface, on Corning TC surface, or on Nunclon Delta surface after previous growth on Corning TC surface

PC-3 cell viability and growth with Nunclon Delta surfaces

  1. Graph (A) shows observations on mean cell viability of PC-3 cells grown on either Nunclon Delta surfaces or Corning TC surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surfaces (for 10 passage doublings) are plotted on the y-axis.
  2. Graph (B) shows mean population doubling time of PC-3 cells propagated on Nunclon Delta surfaces and Corning TC treated surfaces (for 30 population doublings). Those switched to Nunclon Delta surfaces TC treated surface (for 10 passage doublings) are plotted on the y-axis.
Bar charts showing PC-3 cell viability data and population data when grown on Nunclon Delta or on Corning TC surface

Note: Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

PC-3 transfection efficiency with Nunclon Delta surfaces

PC-3 cells seeded on Nunclon Delta surfaces or Corning tissue culture (TC) treated surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.

Please note: Flow cytometry evaluation of transfection efficiency of mCherry in PC-3 cells: 10,000 events were captured per sample. UT=Untransfected cells, T=Transfected cells. n=2.

fluorescence images of HeLa cells grown on Nunclon Delta or on Corning TC surface
flow cytometry histograms of fluorescently stained HeLa cells grown on Nunclon Delta surface or on Corning TC surface

Please note: For the above PC-3 Nunclon Delta vs Corning TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

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Nunclon Sphera surface performance data

HepG2 spheroid generation

HepG2 cells and Nunclon Sphera surfaces

HepG2 cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.

In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality HepG2 spheroids, while the other supplier’s product yielded undesirable satellite colonies.

HepG2 cells and spheroid generation

Spheroid formation in Nunclon Sphera and Corning® ULA™ U-bottomed surface: HepG2 cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.

Brightfield microscope views of the spheroids that result from seeding a range of HepG2 cells from 312 to 5000

HepG2 spheroid generation protocol
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LNCaP spheroid generation

LNCaP cells and Nunclon Sphera surfaces

LNCaP cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.

In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality LNCaP spheroids, while the other supplier’s product yielded undesirable satellite colonies.

LNCaP cells and spheroid generation

Spheroid formation in Nunclon Sphera and Corning ULA U-bottomed surface: LNCaP cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.

Brightfield microscope views of the spheroids that result from seeding a range of LNCaP cells from 150 to 10000

LNCaP spheroid generation protocol
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T47D spheroid generation

T47D cells and Nunclon Sphera surfaces

T47D cells grown on Nunclon Sphera surface, in combination with Gibco cell culture media and fetal bovine serum have been proven to yield consistent, quality spheroids.

In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality T47D spheroids, while the other supplier’s product yielded undesirable satellite colonies.

T47D cells and spheroid generation

Spheroid formation in Nunclon Sphera and Corning ULA U-bottomed surface: T47D cells were seeded at the respective densities on Nunclon Sphera and Corning ULA U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=1,000 μm.

Brightfield microscope views of the spheroids that result from seeding a range of TD47 cells from 500 to 10000

T47D spheroid generation protocol
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