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Kits and Reagents for Sanger Sequencing and Fragment Analysis
Applied Biosystems genetic analysis systems are a trusted standard for Sanger sequencing and fragment analysis by capillary electrophoresis—proven through decades of results, including the first sequencing of the human genome and the discovery of genes implicated in diseases like cystic fibrosis.
This same quality and dependability extend to our kits and reagents. We provide optimized, streamlined kits to help you prepare samples for Sanger sequencing and fragment analysis applications on Applied Biosystems genetic analyzers. We are committed to helping you improve the quality of your analyses by providing everything you need for successful separations by capillary electrophoresis (CE). Our kits will keep your lab running at maximum efficiency.
Sanger sequencing
We continue to develop superior Sanger sequencing kits that help provide solutions for all of your applications and templates.
BigDye Terminator v3.1 Cycle Sequencing Kit
The Applied Biosystems BigDye Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
BigDye Terminator v1.1 Cycle Sequencing Kit
The Applied Biosystems BigDye Terminator v1.1 Cycle Sequencing Kit is designed for specialty applications that require optimal base-calling adjacent to the primer, and for sequencing short PCR product templates with rapid electrophoresis run modules.
BigDye Direct Cycle Sequencing Kit
The BigDye Direct Cycle Sequencing Kit simplifies industry-standard Sanger sequencing workflow by combining post-PCR cleanup and cycle sequencing into a single step.
Sanger Sequencing Kit
The Applied Biosystems Sanger Sequencing Kit provides a convenient and affordable solution for preparing sequencing reactions. The kit provides all the reagents needed for PCR cleanup, cycle sequencing, and sequencing product cleanup.
Finding sequencing primers is simple with our Primer Designer tool containing >300,000 predesigned primer pairs for PCR and sequencing.
View predesigned primers for Sanger sequencing ›
To design your own custom primers for PCR and Sanger sequencing, use the Oligo Perfect Designer.
PCR and sequencing primers
From design to synthesis, quality primers are vital to successful results. Use our online Primer Designer Tool to search for the right PCR/Sanger sequencing primer pair from a database of ~650,000 predesigned primer pairs for resequencing the human exome and human mitochondrial genome. Choose from different amplicon lengths to accommodate various research applications and biological sample types.
ExoSAP-IT products offer a unique, one-tube, one-step, enzymatic method for PCR product cleanup. All ExoSAP All ExoSAP-IT reagents provide 100% sample recovery with no loss of PCR products regardless of the fragment size. This PCR cleanup method removes excess primers and dNTPs and does not interfere with downstream applications. Achieve superior results with ExoSAP-IT reagent—improve accuracy with higher yields and full PCR product recovery.
ExoSAP-IT Express
In only 5 minutes, ExoSAP-IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. This enzymatic cleanup method offers precision, increased yield, and a faster turnaround time compared to alternative purification methods, such as spin columns or beads.
Our cleanup kits offer fast, simple purification for DNA sequencing reactions and enable effective and reliable high-throughput removal of excess BigDye terminators from DNA sequencing reactions.
Which sequencing reaction cleanup product is right for you?
| Cleanup method | Description & application | Product |
|---|---|---|
| Reagent-based sequencing template cleanup | Scavenges all unincorporated BigDye terminators. Stabilizes samples before analysis. Best for long and short fragment recovery. | |
| Gel filtration spin columns | Recovers DNA fragments larger than 16 base pairs. Removes > 98% of salts, NTP's and other unwanted low-molecular-weight impurities. |
Learn more about BigDye XTerminator Purification kit ›
Learn more about sequencing reaction clean up ›
Applied Biosystems performance optimized polymers (POP) are the original high-quality running polymers, backed by the Thermo Fisher Scientific quality guarantee that no other provider can match. Every batch of polymer is subjected to extensive functional testing at each stage in the manufacturing process to ensure that every lot meets our stringent specifications.
Applied Biosystems polymers save you set-up time, unnecessary polymer usage and ensure maximum array lifetime. POP polymers are formulated for a wide range of applications and come in various formats to fit into your exact workflow.
Using the right Applied Biosystems polymers can help reduce your re-run rate and enable high-quality, reproducible data. We offer different polymer chemistries for different needs:
For sequencing runs where a small number of base-calls are needed of <400 bp | |
For Sequencing applications when length of read is important and throughput is not a constraint Excellent resolution of nucleotides close to sequencing primer. Primarily used in conjunction with the BigDye Terminator v1.1 Cycle Sequencing Kit, for sequencing short PCR products | |
Sequencing of read lengths up to ~1,000 bp in conjunction with the BigDye Terminator v3.1 Cycle Sequencing Kit |
Fragment analysis
Fragment analysis techniques allow the development of multiple applications on the genetic analyzer. Overview of fragment analysis applications.
We offer several kits for specific Applied Biosystems fragment analysis-based applications.
- SNaPshot Multiplex Kit—primer extension-based method that enables multiplexing up to 10 SNPs (single nucleotide polymorphisms). Use this system to screen and confirm SNPs, assess DNA methylation, and fingerprint BACs
Associated reagent: GeneScan 120 LIZ Size Standard
- Cell line/Human sample authentication kits—simple and economical tools to authenticate your human cells lines, from sample prep to analysis
Choose from dual-labeled Applied Biosystems TaqMan probes and unlabeled oligos for use as PCR primers. TaqMan MGB (minor groove binder), TAMRA, and QSY custom probes are dual-labeled and can be used in all of your real-time PCR research applications.
- Choice of delivered scales
- Suitable for use in any real-time PCR application
- HPLC purification of all TaqMan probes
View custom labeled primers for fragment analysis applications ›
To prepare for capillary electrophoresis, a spectral calibration with corresponding matrix standard to the group of dye you select for labeling your DNA fragments must be performed on your genetic analyzer to accurately detect the dyes with which your primers are labeled. Each unknown sample is mixed with the size standard and Hi-Di Formamide before processing with electrophoresis. Size standards allow sizing of sample peaks and correct for injection variations.
See the following matrix and size standards chart for the recommended Applied Biosystems matrix standard and GeneScan size standard for your workflow.
Selecting the right matrix and size standards
| Dye set used to label DNA fragments | Corresponding matrix standard | Recommended size standard | Possible applications |
| Dye set E5: dR110 (blue), dR6G (green), dTAMRA (yellow), dROX (red | DS-02 | GeneScan 120 LIZ Size Standard (orange) | SNaPshot multiplexing |
| GeneScan 600 LIZ Size Standard v2.0 (orange) | Microsatellite, LOH, chimerism, inter-simple sequence repeat (ISSR), restriction fragment length polymorphism (RFLP), terminal-RFLP (T-RFLP), methylation-sensitive mobility shift assay (MSMSA) | ||
| Dye set D: 6-FAM (blue), HEX (green), NED (yellow) | DS-30 | GeneScan 500 ROX Size Standard (red) | Custom fragment analysis |
| Dye set D: 6-FAM (blue), VIC (green), NED (yellow) | DS-31 | GeneScan 500 ROX Size Standard (red) | Custom fragment analysis |
| Dye set F: 5-FAM (blue), JOE (green), NED (yellow) | DS-32 | GeneScan 500 ROX Size Standard (red) | Microsatellite |
| amplified fragment length polymorphism (AFLP) | |||
| Dye set G5: 6-FAM (blue), VIC (green), NED (yellow), PET (red) | DS-33 | GeneScan 600 LIZ Size Standard v2.0 (orange) | Microsatellite |
| Custom fragment analysis | |||
| Dye set J6: 6-FAM (blue), VIC (green), NED (yellow), SID (violet), TAZ (red) | DS-36 | GeneScan 600 LIZ Size Standard v2.0 (orange) | Custom fragment analysis |
Applied Biosystems performance optimized polymers (POP) are the original high-quality running polymers, backed by the Thermo Fisher Scientific quality guarantee that no other provider can match. Every batch of polymer is subjected to extensive functional testing at each stage in the manufacturing process to ensure that every lot meets our stringent specifications.
Applied Biosystems polymers save you set-up time, unnecessary polymer usage and ensure maximum array lifetime. POP polymers are formulated for a wide range of applications and come in various formats to fit into your exact workflow.
Using the right Applied Biosystems polymers can help reduce your re-run rate and enable high-quality, reproducible data. We offer different polymer chemistries for different needs:
For human identification applications, other fragment analysis applications, short DNA fragments (<500 bp) | |
For mid-sized DNA fragments (500-700 bp) | |
For long DNA fragments (>700 bp) |