PsiIAanI (PsiI)Optimal Buffer: Tango
TTA^TAA
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
AarIOptimal Buffer: Unique (+oligo)
CACCTGC(4/8)^
B | NR |
---|
G | NR |
---|
O | 0-20 (+oligo) |
---|
R | 0-20 (+oligo) |
---|
1X Tango | NR (+oligo) |
---|
2X Tango | 50-100 (+oligo) |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X (+oligo) |
---|
Overnight | 1 |
---|
Inactivation | 65° C |
---|
AatIIOptimal Buffer: Tango
GACGT^C
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
Asp718IAcc65I (Asp718I)Optimal Buffer: O
G^GTACC
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 20-50 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
DraIIIAdeI (DraIII)Optimal Buffer: G
CACNNN^GTG
B | 0-20 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 100 |
---|
1X Tango | 100* |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | NO |
---|
BmgBIAjiI (BmgBI)Optimal Buffer: Unique
CAC^GTC
B | NR |
---|
G | NR |
---|
O | 20-50* |
---|
R | NR |
---|
1X Tango | NR |
---|
2X Tango | 20-50* |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X* |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
AjuIOptimal Buffer: R (+SAM)
^(7/12)GAA(N)7TTGG(11/6)^
B | 0-20 (+SAM) |
---|
G | 50-100 (+SAM) |
---|
O | 20-50 (+SAM) |
---|
R | 100 (+SAM) |
---|
1X Tango | 50-100 (+SAM) |
---|
2X Tango | 50-100 (+SAM) |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X (+SAM) |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
AloIOptimal Buffer: R
^(7/12-13)GAAC(N)6TCC(12-13/7)^
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 30°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
AluIOptimal Buffer: Tango
AG^CT
B | 50-100 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BsiHKAIAlw21I (BsiHKAI)Optimal Buffer: O
GWGCW^C
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BsmAIAlw26I (BsmAI)Optimal Buffer: Tango
GTCTC(1/5)^
B | 50-100 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
ApaLIAlw44I (ApaLI)Optimal Buffer: Tango
G^TGCAC
B | 50-100 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
ApaIOptimal Buffer: B
GGGCC^C
B | 100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 20-50 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
BamHIOptimal Buffer: Unique
G^GATCC
B | 20-50* |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 50-100* |
---|
1X Tango | 100* |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C (10 u) |
---|
BssSIBauI (BssSI)Optimal Buffer: Tango
CACGAG(-5/-1)^
B | 0-20 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
BclIOptimal Buffer: G
T^GATCA
B | 20-50 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100* |
---|
2X Tango | 100 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X* or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C (10 u) |
---|
NciIBcnI (NciI)Optimal Buffer: Tango
CC^SGG
B | 20-50 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
SpeIBcuI (SpeI)Optimal Buffer: Tango
A^CTAGT
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | NO |
---|
SfcIBfmI (SfcI)Optimal Buffer: Tango
C^TRYAG
B | 0-20 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 1 |
---|
Inactivation | 65° C |
---|
BciVIBfuI (BciVI)Optimal Buffer: Unique
GTATCC(6/5)^
B | NR |
---|
G | NR |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | NR |
---|
2X Tango | 50-100* |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X* |
---|
Overnight | 1 |
---|
Inactivation | 80° C |
---|
BglIOptimal Buffer: O
GCCNNNN^NGGC
B | 0-20 |
---|
G | 50-100 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BglIIOptimal Buffer: O
A^GATCT
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | NO |
---|
ScrFIBme1390I (ScrFI)Optimal Buffer: O
CC^NGG
B | 20-50 |
---|
G | 50-100 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
PshAIBoxI (PshAI)Optimal Buffer: Tango
GACNN^NNGTC
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C |
---|
BbsIBpiI (BbsI)Optimal Buffer: G
GAAGAC(2/6)^
B | 20-50 |
---|
G | 100 |
---|
O | 50-100 |
---|
R | 50-100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
BplIOptimal Buffer: Tango(+SAM)
^(8/13)GAG(N)5CTC(13/8)^
B | 0-20 (+SAM) |
---|
G | 20-50 (+SAM) |
---|
O | 0-20 (+SAM) |
---|
R | 0-20 (+SAM) |
---|
1X Tango | 100 (+SAM) |
---|
2X Tango | 20-50 (+SAM) |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X (+SAM) |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
Bpu10IOptimal Buffer: Unique
CCTNAGC(-5/-2)^
B | 0-20 |
---|
G | 20-50* |
---|
O | 50-100* |
---|
R | 100* |
---|
1X Tango | 50-100* |
---|
2X Tango | 100* |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X* |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
BlpIBpu1102I (BlpI)Optimal Buffer: Tango
GC^TNAGC
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
BsaJIBseDI (BsaJI)Optimal Buffer: Tango
C^CNNGG
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
BtsCIBseGI (BtsCI)Optimal Buffer: Tango
GGATG(2/0)^
B | 20-50 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
BsaBIBseJI (BsaBI)Optimal Buffer: O
GATNN^NNATC
B | NR |
---|
G | 100* |
---|
O | 100 |
---|
R | NR |
---|
1X Tango | 100* |
---|
2X Tango | 100* |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 2X* |
---|
Overnight | 0.1 |
---|
Inactivation | NO |
---|
BslIBseLI (BslI)Optimal Buffer: Tango
CCNNNNN^NNGG
B | 20-50 |
---|
G | 100 |
---|
O | 50-100 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | NO |
---|
BsrDIBseMI (BsrDI)Optimal Buffer: R
GCAATG(2/0)^
B | 0-20 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 80° C |
---|
BspCNIBseMII (BspCNI)Optimal Buffer: Tango(+SAM)
CTCAG(10/8)^
B | 50-100 (+SAM) |
---|
G | 50-100 (+SAM) |
---|
O | 50-100 (+SAM) |
---|
R | 50-100 (+SAM) |
---|
1X Tango | 100 (+SAM) |
---|
2X Tango | 50-100 (+SAM) |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X (+SAM) |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C |
---|
BsrIBseNI (BsrI)Optimal Buffer: B
ACTGG(1/-1)^
B | 100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
Bme1580IBseSI (Bme1580I)Optimal Buffer: G
GKGCM^C
B | 20-50 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C (10 u) |
---|
BbvIBseXI (BbvI)Optimal Buffer: Unique
GCAGC(8/12)^
B | NR |
---|
G | NR |
---|
O | NR |
---|
R | NR |
---|
1X Tango | NR |
---|
2X Tango | NR |
---|
Optimal Temperature | 65°C |
---|
Double Digest | NR |
---|
Overnight | 0.3 |
---|
Inactivation | 80° C |
---|
BstUIBsh1236I (BstUI)Optimal Buffer: R
CG^CG
B | 0-20 |
---|
G | 0-20 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65°C |
---|
BsiEIBsh1285I (BsiEI)Optimal Buffer: G
CGRY^CG
B | 20-50 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 0-20 |
---|
1X Tango | 0-20 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
BanIBshNI (BanI)Optimal Buffer: O
G^GYRCC
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
AgeIBshTI (AgeI)Optimal Buffer: O
A^CCGGT
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
BstBIBsp119I (BstBI)Optimal Buffer: Tango
TT^CGAA
B | 20-50 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
PspOMIBsp120I (PspOMI)Optimal Buffer: B
G^GGCCC
B | 100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
BsrGIBsp1407I (BsrGI)Optimal Buffer: Tango
T^GTACA
B | 0-20 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
Sau3AIBsp143I (Sau3AI)Optimal Buffer: Unique
^GATC
B | 20-50 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
NruIBsp68I (NruI)Optimal Buffer: O
TCG^CGA
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
NlaIVBspLI (NlaIV)Optimal Buffer: Tango
GGN^NCC
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
BmtIBspOI (BmtI)Optimal Buffer: O
GCTAG^C
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
AlwIBspPI (AlwI)Optimal Buffer: Tango
GGATC(4/5)^
B | 20-50 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C |
---|
AflIIBspTI (AflII)Optimal Buffer: O
C^TTAAG
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 20-50 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BstZ17IBst1107I (BstZ17I)Optimal Buffer: O
GTA^TAC
B | 20-50 |
---|
G | 50-100 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
BstXIOptimal Buffer: O
CCANNNNN^NTGG
B | 20-50 |
---|
G | 100 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
ClaIBsu15I (ClaI)Optimal Buffer: Tango
AT^CGAT
B | 20-50 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
HaeIIIBsuRI (HaeIII)Optimal Buffer: R
GG^CC
B | 20-50 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
BspMIBveI (BspMI)Optimal Buffer: O (+oligo)
ACCTGC(4/8)^
B | 0-20 (+oligo) |
---|
G | 20-50 (+oligo) |
---|
O | 100 (+oligo) |
---|
R | 20-50 (+oligo) |
---|
1X Tango | 50-100 (+oligo) |
---|
2X Tango | 100 (+oligo) |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X (+oligo) |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
AlwNICaiI (AlwNI)Optimal Buffer: Tango
CAGNNN^CTG
B | 20-50 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
BsrFICfr10I (BsrFI)Optimal Buffer: Unique
R^CCGGY
B | 0-20 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 50-100* |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | NO |
---|
Sau96ICfr13I (Sau96I)Optimal Buffer: Tango
G^GNCC
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
SacIICfr42I (SacII)Optimal Buffer: B
CCGC^GG
B | 100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
XmaICfr9I (XmaI)Optimal Buffer: Unique
C^CCGGG
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 20-50 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
RsrIICpoI (RsrII)Optimal Buffer: Tango
CG^GWCCG
B | 20-50 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
HgaICseI (HgaI)Optimal Buffer: R
GACGC(5/10)^
B | NR |
---|
G | 50-100* |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 100* |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C (10 u) |
---|
CviQICsp6I (CviQI)Optimal Buffer: B
G^TAC
B | 100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
DpnIOptimal Buffer: Tango
Gm6A^TC
B | 100 |
---|
G | 100 |
---|
O | 50-100 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
DraIOptimal Buffer: Tango
TTT^AAA
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
EarIEam1104I (EarI)Optimal Buffer: Tango
CTCTTC(1/4)^
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
AhdIEam1105I (AhdI)Optimal Buffer: Unique
GACNNN^NNGTC
B | 20-50 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
EcoICRIEcl136II (EcoICRI)Optimal Buffer: Unique
GAG^CTC
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
SnaBIEco105I (SnaBI)Optimal Buffer: Tango
TAC^GTA
B | 100* |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
StyIEco130I (StyI)Optimal Buffer: O
C^CWWGG
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
StuIEco147I (StuI)Optimal Buffer: B
AGG^CCT
B | 100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
BanIIEco24I (BanII)Optimal Buffer: Tango
GRGCY^C
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
BsaIEco31I (BsaI)Optimal Buffer: G
GGTCTC(1/5)^
B | 50-100 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
EcoRVEco32I (EcoRV)Optimal Buffer: R
GAT^ATC
B | 0-20 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
AvaIIEco47I (AvaII)Optimal Buffer: R
G^GWCC
B | 0-20 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
AfeIEco47III (AfeI)Optimal Buffer: O
AGC^GCT
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
EagIEco52I (EagI)Optimal Buffer: Unique
C^GGCCG
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 0-20 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
AcuIEco57I (AcuI)Optimal Buffer: G (+SAM)
CTGAAG(16/14)^
B | 100 (+SAM) |
---|
G | 100 (+SAM) |
---|
O | 20-50 (+SAM) |
---|
R | 20-50 (+SAM) |
---|
1X Tango | 50-100 (+SAM) |
---|
2X Tango | 50-100 (+SAM) |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X (+SAM) |
---|
Overnight | 1 |
---|
Inactivation | 65° C |
---|
PmlIEco72I (PmlI)Optimal Buffer: Tango
CAC^GTG
B | NR |
---|
G | NR |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
Bsu36IEco81I (Bsu36I)Optimal Buffer: Tango
CC^TNAGG
B | 50-100 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
AvaIEco88I (AvaI)Optimal Buffer: Tango
C^YCGRG
B | 100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
BstEIIEco91I (BstEII)Optimal Buffer: O
G^GTNACC
B | 20-50 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | NR |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
DraIIEcoO109I (DraII)Optimal Buffer: Tango
RG^GNCCY
B | 50-100 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
EcoRIOptimal Buffer: Unique
G^AATTC
B | 0-20 |
---|
G | NR |
---|
O | 100 |
---|
R | 100* |
---|
1X Tango | NR |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
EcoRIIOptimal Buffer: O
^CCWGG
B | 20-50 |
---|
G | 50-100 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
SfoIEheI (SfoI)Optimal Buffer: Tango
GGC^GCC
B | 20-50 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 1 |
---|
Inactivation | 65° C |
---|
BsmBIEsp3I (BsmBI)Optimal Buffer: Tango (+DTT)
CGTCTC(1/5)^
B | 100 (+DTT) |
---|
G | 20-50 (+DTT) |
---|
O | 0-20 (+DTT) |
---|
R | 0-20 (+DTT) |
---|
1X Tango | 100 (+DTT) |
---|
2X Tango | 0-20 (+DTT) |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X (+DTT) |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
BsmFIFaqI (BsmFI)Optimal Buffer: Tango (+SAM)
GGGAC(10/14)^
B | 20-50 (+SAM) |
---|
G | 20-50 (+SAM) |
---|
O | 0-20 (+SAM) |
---|
R | 0-20 (+SAM) |
---|
1X Tango | 100 (+SAM) |
---|
2X Tango | 50-100 (+SAM) |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X (+SAM) |
---|
Overnight | 1 |
---|
Inactivation | 80° C |
---|
FspAIOptimal Buffer: O
RTGC^GCAY
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
BfaIFspBI (BfaI)Optimal Buffer: Tango
C^TAG
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
BpmIGsuI (BpmI)Optimal Buffer: B
CTGGAG(16/14)^
B | 100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 30°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 1 |
---|
Inactivation | 65° C |
---|
HhaIOptimal Buffer: Tango
GCG^C
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | NO |
---|
BsaHIHin1I (BsaHI)Optimal Buffer: G
GR^CGYC
B | 20-50 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 20-50 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
NlaIIIHin1II (NlaIII)Optimal Buffer: G
CATG^
B | 50-100 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 50-100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
HinP1IHin6I (HinP1I)Optimal Buffer: Tango
G^CGC
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
HindIIHincII (HindII)Optimal Buffer: Tango
GTY^RAC
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
HindIIIOptimal Buffer: R
A^AGCTT
B | 0-20 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
HinfIOptimal Buffer: R
G^ANTC
B | 0-20 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
HpaIIOptimal Buffer: Tango
C^CGG
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
HphIOptimal Buffer: B
GGTGA(8/7)^
B | 100 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 20-50 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
MjaIVHpy8I (MjaIV)Optimal Buffer: Tango
GTN^NAC
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
MwoIHpyF10VI (MwoI)Optimal Buffer: Tango
GCNNNNN^NNGC
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
DdeIHpyF3I (DdeI)Optimal Buffer: Tango
C^TNAG
B | 20-50 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
I-SceIOptimal Buffer: Tango
TAGGG ATAA^CAGGGTAATATCCC?TATT GTCCCATTA
B | 50-100 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
BspEIKpn2I (BspEI)Optimal Buffer: Tango
T^CCGGA
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
KpnIOptimal Buffer: Unique
GGTAC^C
B | 20-50 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 20-50 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
HpaIKspAI (HpaI)Optimal Buffer: B
GTT^AAC
B | 100 |
---|
G | 50-100* |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100* |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
SapILguI (SapI)Optimal Buffer: Tango
GCTCTTC(1/4)^
B | 20-50 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
BbvILsp1109I (BbvI)Optimal Buffer: Unique
GCAGC(8/12)^
B | 0-20 |
---|
G | 20-50* |
---|
O | 50-100* |
---|
R | 100* |
---|
1X Tango | 20-50* |
---|
2X Tango | 20-50* |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X* |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
SfaNILweI (SfaNI)Optimal Buffer: Tango
GCATC(5/9)^
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
MauBIOptimal Buffer: Tango
CG^CGCGCG
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BsrBIMbiI (BsrBI)Optimal Buffer: Tango
CCGCTC(-3/-3)^
B | 20-50 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
MboIOptimal Buffer: R
^GATC
B | 50-100 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
MboIIOptimal Buffer: B
GAAGA(8/7)^
B | 100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 1 |
---|
Inactivation | 65° C |
---|
MscIMlsI (MscI)Optimal Buffer: R
TGG^CCA
B | 0-20 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
MluIOptimal Buffer: R
A^CGCGT
B | 0-20 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
MnlIOptimal Buffer: G
CCTC(7/6)^
B | 50-100 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 20-50 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
NsiIMph1103I (NsiI)Optimal Buffer: R
ATGCA^T
B | 0-20 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
Sse232IMreI (Sse232I)Optimal Buffer: G
CG^CCGGCG
B | 20-50 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C |
---|
HpaIIMspI (HpaII)Optimal Buffer: Tango
C^CGG
B | 50-100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 80° C |
---|
PmeIMssI (PmeI)Optimal Buffer: B
GTTT^AAAC
B | 100 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 20-50 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
MfeIMunI (MfeI)Optimal Buffer: G
C^AATTG
B | 100 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BsmIMva1269I (BsmI)Optimal Buffer: R
GAATGC(1/-1)^
B | 0-20 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BstNIMvaI (BstNI)Optimal Buffer: R
CC^WGG
B | 20-50 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50* |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | NO |
---|
Nb.Bpu10IOptimal Buffer: R
CCTNA GCGGANT?CG
B | 0-20 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 80° C |
---|
Nb.Mva1269IOptimal Buffer: O
GAATG CCTTAC?G
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 80° C |
---|
NcoIOptimal Buffer: Tango
C^CATGG
B | 20-50 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
NdeIOptimal Buffer: O
CA^TATG
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
NheIOptimal Buffer: Tango
G^CTAGC
B | 100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
Tsp45INmuCI (Tsp45I)Optimal Buffer: R
^GTSAC
B | 0-20 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
NotIOptimal Buffer: O
GC^GGCCGC
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 20-50 |
---|
1X Tango | 0-20 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
FspINsbI (FspI)Optimal Buffer: Tango
TGC^GCA
B | 20-50 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
Nt.Bpu10IOptimal Buffer: R
CC^TNAGCGG ANTCG
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
AleIOliI (AleI)Optimal Buffer: R
CACNN^NNGTG
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
PacIOptimal Buffer: Unique
TTAAT^TAA
B | 20-50 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 0-20 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | NR |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
SphIPaeI (SphI)Optimal Buffer: B
GCATG^C
B | 100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BspHIPagI (BspHI)Optimal Buffer: O
T^CATGA
B | 0-20 |
---|
G | 50-100 |
---|
O | 100 |
---|
R | NR |
---|
1X Tango | NR |
---|
2X Tango | NR |
---|
Optimal Temperature | 37°C |
---|
Double Digest | NR |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
PasIOptimal Buffer: Unique
CC^CWGGG
B | NR |
---|
G | NR |
---|
O | NR |
---|
R | NR |
---|
1X Tango | NR |
---|
2X Tango | NR |
---|
Optimal Temperature | 55°C |
---|
Double Digest | NR |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
BssHIIPauI (BssHII)Optimal Buffer: R
G^CGCGC
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 0-20 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
NaeIPdiI (NaeI)Optimal Buffer: Tango
GCC^GGC
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65°C |
---|
XmnIPdmI (XmnI)Optimal Buffer: Tango
GAANN^NNTTC
B | 20-50 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
TfiIPfeI (TfiI)Optimal Buffer: O
G^AWTC
B | 0-20 |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BsiWIPfl23II (BsiWI)Optimal Buffer: Tango
C^GTACG
B | 20-50 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
PfoIOptimal Buffer: Tango
T^CCNGGA
B | 0-20 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
BsaAIPpu21I (BsaAI)Optimal Buffer: Unique
YAC^GTR
B | 50-100* |
---|
G | 100* |
---|
O | 20-50 |
---|
R | NR |
---|
1X Tango | NR |
---|
2X Tango | NR |
---|
Optimal Temperature | 30°C |
---|
Double Digest | NR |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
PciIPscI (PciI)Optimal Buffer: Tango
A^CATGT
B | 20-50 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
AclIPsp1406I (AclI)Optimal Buffer: Tango
AA^CGTT
B | 100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
PpuMIPsp5II (PpuMI)Optimal Buffer: G
RG^GWCCY
B | 0-20 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
PstIOptimal Buffer: O
CTGCA^G
B | 50-100 |
---|
G | 50-100 |
---|
O | 100 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | NO |
---|
BstYIPsuI (BstYI)Optimal Buffer: B
R^GATCY
B | 100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C |
---|
Tth111IPsyI (Tth111I)Optimal Buffer: B
GACN^NNGTC
B | 100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
PvuIOptimal Buffer: R
CGAT^CG
B | 0-20 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C (10 u) |
---|
PvuIIOptimal Buffer: G
CAG^CTG
B | 50-100* |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 50-100 |
---|
1X Tango | 20-50* |
---|
2X Tango | 20-50* |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X* or 2X* |
---|
Overnight | 0.2 |
---|
Inactivation | NO |
---|
RsaIOptimal Buffer: Tango
GT^AC
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
MslIRseI (MslI)Optimal Buffer: R
CAYNN^NNRTG
B | 0-20 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
SacIOptimal Buffer: Unique
GAGCT^C
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
SalIOptimal Buffer: O
G^TCGAC
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 20-50 |
---|
1X Tango | 0-20 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
Fnu4HISatI (Fnu4HI)Optimal Buffer: G
GC^NGC
B | 20-50 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
ScaIOptimal Buffer: Unique
AGT^ACT
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 0-20 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | NR |
---|
Overnight | 0.5 |
---|
Inactivation | 80° C (10 u) |
---|
MlyISchI (MlyI)Optimal Buffer: Tango
GAGTC(5/5)^
B | 20-50 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
SbfISdaI (SbfI)Optimal Buffer: Unique
CCTGCA^GG
B | NR |
---|
G | NR |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | NR |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 80° C |
---|
Bsp1286ISduI (Bsp1286I)Optimal Buffer: Unique
GDGCH^C
B | NR |
---|
G | 50-100* |
---|
O | 50-100 |
---|
R | 0-20 |
---|
1X Tango | NR |
---|
2X Tango | NR |
---|
Optimal Temperature | 37°C |
---|
Double Digest | NR |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
AsiSISfaAI (AsiSI)Optimal Buffer: Tango
GCGAT^CGC
B | 50-100 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
SfiIOptimal Buffer: G
GGCCNNNN^NGGCC
B | 50-100 |
---|
G | 100 |
---|
O | 20-50 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 50°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | NO |
---|
SgeIOptimal Buffer: Unique
m5CNNG(9/13)^
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | NR |
---|
1X Tango | NR |
---|
2X Tango | NR |
---|
Optimal Temperature | 37°C |
---|
Double Digest | NR |
---|
Overnight | NR |
---|
Inactivation | 65° C |
---|
SgrDIOptimal Buffer: R
CG^TCGACG
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 100 |
---|
1X Tango | NR |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.3 |
---|
Inactivation | 65° C |
---|
AscISgsI (AscI)Optimal Buffer: Tango
GG^CGCGCC
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
SmaIOptimal Buffer: Tango
CCC^GGG
B | 50-100 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 30°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
SwaISmiI (SwaI)Optimal Buffer: O
ATTT^AAAT
B | 0-20 |
---|
G | 0-20 |
---|
O | 100 |
---|
R | 20-50 |
---|
1X Tango | 0-20 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 30°C |
---|
Double Digest | 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
SmlISmoI (SmlI)Optimal Buffer: Tango
C^TYRAG
B | 50-100 |
---|
G | 20-50 |
---|
O | 0-20 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
AciISsiI (AciI)Optimal Buffer: O
CCGC(-3/-1)^
B | NR |
---|
G | 20-50 |
---|
O | 100 |
---|
R | 50-100 |
---|
1X Tango | NR |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 2X |
---|
Overnight | 0.5 |
---|
Inactivation | 65° C |
---|
KasISspDI (KasI)Optimal Buffer: Tango
G^GCGCC
B | 20-50 |
---|
G | 0-20 |
---|
O | NR |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
SspIOptimal Buffer: G
AAT^ATT
B | 20-50 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
HpyCH4IIITaaI (HpyCH4III)Optimal Buffer: Tango
ACN^GT
B | 0-20 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | NO |
---|
MaeIITaiI (MaeII)Optimal Buffer: R
ACGT^
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | NO |
---|
TaqIOptimal Buffer: Unique
T^CGA
B | 0-20 |
---|
G | 20-50 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 20-50 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.3 |
---|
Inactivation | NO |
---|
Tsp509ITasI (Tsp509I)Optimal Buffer: B
^AATT
B | 100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 0-20 |
---|
1X Tango | 20-50 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X |
---|
Overnight | 0.3 |
---|
Inactivation | NO |
---|
TatIOptimal Buffer: Tango
W^GTACW
B | NR |
---|
G | 50-100* |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100* |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X* |
---|
Overnight | 0.2 |
---|
Inactivation | NO |
---|
TauIOptimal Buffer: B
GCSG^C
B | 100 |
---|
G | 50-100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 20-50 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 55°C |
---|
Double Digest | 1X |
---|
Overnight | 1 |
---|
Inactivation | NO |
---|
MseITru1I (MseI)Optimal Buffer: R
T^TAA
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 100 |
---|
1X Tango | 50-100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | NO |
---|
TspRITscAI (TspRI)Optimal Buffer: Tango
CASTG(2/-7)^
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 65°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | NO |
---|
PflMIVan91I (PflMI)Optimal Buffer: R
CCANNNN^NTGG
B | 0-20 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
AseIVspI (AseI)Optimal Buffer: O
AT^TAAT
B | 0-20 |
---|
G | 50-100 |
---|
O | 100 |
---|
R | 20-50 |
---|
1X Tango | 100 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
EcoNIXagI (EcoNI)Optimal Buffer: R
CCTNN^NNNAGG
B | 0-20 |
---|
G | 20-50 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65° C |
---|
ApoIXapI (ApoI)Optimal Buffer: Tango
R^AATTY
B | 50-100 |
---|
G | 100 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
XbaIOptimal Buffer: Tango
T^CTAGA
B | 50-100 |
---|
G | 50-100 |
---|
O | 20-50 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65°C |
---|
NspIXceI (NspI)Optimal Buffer: Tango
RCATG^Y
B | 50-100 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 100 |
---|
2X Tango | 0-20 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X |
---|
Overnight | 0.2 |
---|
Inactivation | 65° C |
---|
XhoIOptimal Buffer: R
C^TCGAG
B | 0-20 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 100 |
---|
1X Tango | 20-50 |
---|
2X Tango | 100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 80° C |
---|
AvrIIXmaJI (AvrII)Optimal Buffer: Tango
C^CTAGG
B | 20-50 |
---|
G | 50-100 |
---|
O | 50-100 |
---|
R | 50-100 |
---|
1X Tango | 100 |
---|
2X Tango | 50-100 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.2 |
---|
Inactivation | 80° C |
---|
AccIXmiI (AccI)Optimal Buffer: B
GT^MKAC
B | 100 |
---|
G | 0-20 |
---|
O | 0-20 |
---|
R | 0-20 |
---|
1X Tango | 50-100 |
---|
2X Tango | 20-50 |
---|
Optimal Temperature | 37°C |
---|
Double Digest | 1X or 2X |
---|
Overnight | 0.1 |
---|
Inactivation | 65°C |
---|
Notes
* Star activity appears at a geater than 5-fold overdigestion (5 units x 1 hour).
NR - buffer is not recommended, because of high star activity.
80°C (10U) - only small amounts of the restriction enzyme (up to 10 units) can be inactivated at 80°C in 20 minutes.