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Targeted Genotyping by Sequencing
Targeted genotyping by sequencing (GBS) is the latest innovation in genomics-assisted breeding programs. Targeted GBS uses a powerful multiplexed approach that enables users to interrogate hundreds to thousands of markers across hundreds to thousands of samples simultaneously. It includes NGS-based assays designed using customer-provided markers or catalog panels designed with publicly available markers.
Applications
- Marker-assisted selection and breeding
- Parentage and traceability
Benefits
- Fast turnaround time
- Cost effective
- Simplified data analysis
Thermo Fisher Scientific offers two targeted genotyping by sequencing solutions:
- Applied Biosystems AgriSeq targeted GBS solutions
The table below highlights the key features of our Applied Biosystems AgriSeq and targeted GBS technology.
| AgriSeq targeted GBS solutions | |
|---|---|
| Requires Ion Torrent instrument | Yes |
| SNPs per panel | 50–5,000 |
| Ability to discover new variants | Yes |
| Expected call rates* | –95% |
| Processing time | 2 days |
| Panel design | 2–6 weeks for custom panel design |
| Sample throughput/week | Up to 7,680** |
| Cost per sample | Low |
* Depends on level of genome annotation. ** Based on panel size with one instrument and one technician.