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Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A/G is a recombinant protein expressed in Escherichia coli (50.5 kDa; 6 IgG binding sites) and is recommended for purifying polyclonal IgG from many species. The POROS MabCapture A/G Select Resin is a new product that can be scaled up for bioprocess applications.
We offer a wide selection of base supports and formats to purify antibodies using the Protein A/G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.
Product links in the last row of each column
| Pierce Protein A/G Magnetic Beads | Pierce Protein A/G Magnetic Agarose Beads | Pierce Protein A/G Agarose | Pierce Protein A/G Plus Agarose | POROS MabCapture A/G Select | |
| Bead or resin size | 1 μm | 10–40 μm | 45–165 μm | 45–165 μm | 50 μm |
| Binding capacity | ≥55–85 µg human IgG/mg beads | ≥40 mg rabbit IgG/mL beads | ≥7 mg human IgG/mL resin | ≥50 mg human IgG/mL resin | ≥22 mg/mL human IgG/mL resin |
| Maximum linear flow rate | N/A | N/A | 700 cm/hr | 700 cm/hr | 3000 cm/hr |
| Support | Magnetite-coated polymer | Magnetite-embedded beaded agarose | Crosslinked 6% beaded agarose | Crosslinked 6% beaded agarose | Polystyrene-divinylbenzene |
| Recommended application scale | screening | high-throughput batch | batch, pilot | batch, pilot | batch, pilot, process |
| Formats available | loose beads | loose beads | loose beads, pre-packed spin columns and kits, chromatography cartridges | loose beads | loose beads |
| Order product(s) | 88802 | 78609 | 20421 (loose resin) 89930 (1 mL cartridge) 89931 (5 mL cartridge) | 20423 | 82082 |