Ion Indicators and Ion Channels Information

Learn about assays and techniques for detecting and monitoring ion channels and ion flux using fluorescent ion indicators by flow cytometry, imaging microscopy, high-content analysis and microplate assays. Topics include calcium and magnesium indicators, as well probes for potassium channel flux.

Ion indicators and ion channels features

BioProbes article

FluxOR II Green Potassium Ion Channel Assay—Next-generation detection of potassium ion flux

Potassium channels are ion-selective protein pores that span the cell’s plasma membrane and serve to establish and regulate membrane potential. In excitable cells such as neurons and myocytes, these channels function both to shape the action potential and to reset the cell’s resting membrane potential. The Invitrogen FluxOR II Green Potassium Ion Channel Assay is the newest tool for high-throughput detection of potassium ion channel and transporter activities.


Ion indicators and ion channels learning resources

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TypeTitleCategories
BioProbes articles (Issues 50– present day)BioProbes Journal of Cell Biology Applicationcell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Molecular Probes HandbookMAXC Computer Program for calculating Free Ca2+ concentrations—Note 19.2ion channels, ion flux, ion indicators
Molecular Probes HandbookLoading and calibration of intracellular ion indicators—Note 19.1ion channels, ion flux, ion indicators
Molecular Probes HandbookCalcium regulation—Section 17.2ion channels, ion flux, ion indicators
Molecular Probes HandbookProbes for ion channels and carriers—Section 16.3ion channels, ion flux, ion indicators
Molecular Probes HandbookProbes useful at near-neutral pH—Section 20.2BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes HandbookProbes useful at acidic pH—Section 20.3flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookpH indicator conjugates—Section 20.4flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookOverview of pH indicators—Section 20.1flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes HandbookFluorescent Na+ and K+ indicators—Section 21.1ion channels, ion flux, ion indicators
Molecular Probes HandbookDetecting chloride, phosphate, nitrite and other anions—Section 21.2ion channels, ion flux, ion indicators
Molecular Probes HandbookIntroduction to Ca2+ measurements with fluorescent indicators—Section 19.1ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Mg2+ indicators—Section 19.6ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent indicators for Zn2+ and other metal ions—Section 19.7ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Ca2+ indicators excited with visible light—Section 19.3ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Ca2+ indicators excited with UV light—Section 19.2ion channels, ion flux, ion indicators
Molecular Probes HandbookFluorescent Ca2+ indicator conjugates—Section 19.4ion channels, ion flux, ion indicators
Molecular Probes HandbookChelators, calibration buffers, ionophores and cell-loading reagents—Section 19.8ion channels, ion flux, ion indicators
Molecular Probes HandbookProtein-based Ca2+ indicators—Section 19.5calcium flux, fluorescent dyes, ion indicators
Scientific poster (2008)Living molecular probes for the next generation BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging
Scientific poster (2008)Cell-based potassium ion channel screening with the FluxOR Assay BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008)Improving downstream results with automated cell counting automated cell counter, BacMam technology, cell counting, cell expansion, cell isolation, Countess, fluorescent dyes, hemocytometer, ion flux/ion channels, microplate reader
Scientific poster (2008)FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2009)Cell-based potassium ion channel screening with BacMam using the FluxOR Assay BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2010)A new generation of cellular imaging probes for neurosciences research autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010)BacMam gene expression for functional and imaging applications BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
VideoLIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System 
Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.
cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging

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