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单细胞分析
Uncover critical variations
It was once assumed that cell populations were homogeneous, but the latest evidence shows that heterogeneity does in fact exist even within small cell populations. Gene expression measurements based on the homogenized cell population are misleading averages and don't account for the small but critical changes occurring in individual cells. Individual cells can differ dramatically in size, protein levels, and expressed RNA transcripts, and these variations are key to answering previously unsolvable questions in cancer research, stem cell biology, immunology, developmental biology, and neurology.
We offer analysis tools that can be used throughout various single-cell workflows for cellular biologists and researchers performing a wide spectrum of basic, translational, and clinical research projects.
With single-cell analysis you can:
- Unlock the mystery in gene expression profiles between individual cells
- Avoid the mistake of taking averages of entire cell populations
- Discover previously undetected subpopulations and unveil new regulatory path
View our newest application note on single cell analysis
单细胞分析的关键应用是基因表达
我们已使用这些单细胞基因表达产品证实了一些工作流程：
- Ambion Single Cell-to-CT™ 试剂盒
- 可靠的 TaqMan 测定
- QuantStudio™ 12K Flex 实时 PCR 系统
图 1.Single Cell-to-CT™ 实时 PCR 工作流。
下载我们的关于单细胞基因表达异质性的应用说明
对单细胞分析非常重要的其他应用
稀有细胞或事件
| 罕见珍贵的样本
| 群体中的单细胞精确性
|
推荐的单细胞分析产品
EVOS Cell Imaging Systems
Visualize single cells with florescence imaging accessible to almost every lab and budget
Attune Flow Cytometer
Sensitive cell analysis in a high-throughput benchtop flow cytometer
Single Cells-to-CT kit
A validated workflow for gene expression analysis with a standardized platform for studying single cells
Ion PGM sequencer
Ion semiconductor sequencing makes high-quality sequencing possible on single cells
激光捕获显微切割
该方法特别温和，是单细胞或少量细胞显微切割的理想之选
QuantStudio™ 12K Flex
使用实时 PCR OpenArray 技术进行单细胞的基因表达检测分析
网络讲座&视频
 | 网络讲座：采用 Single Cell-to-Ct™ 试剂盒进行表达谱分析 | |
 | 视频：使用 Ambion® Cells-to-CT™ 试剂盒进行实时 PCR，更省时 通常来说，实时 PCR 的第一步是抽提RNA。抽提步骤可能需要很长的时间，包括多个处理步骤，还有损失样本的风险。Ambion® Cells-to-CT™ 试剂盒提供了简单快捷的细胞裂解制备方法。 | |
 | 视频：Cells to CT™ 试剂盒展示 在这项展示中，Richard Fekete 博士使用 Cells-to-Cт™ 试剂盒在 10 分钟内制备了 96 份样品，并将此过程与传统离心柱的 RNA 提纯方法进行了对比。 |
网络讲座&视频
| 网络讲座：采用 Single Cell-to-Ct™ 试剂盒进行表达谱分析 | |
| 视频：使用 Ambion® Cells-to-CT™ 试剂盒进行实时 PCR，更省时 通常来说，实时 PCR 的第一步是抽提RNA。抽提步骤可能需要很长的时间，包括多个处理步骤，还有损失样本的风险。Ambion® Cells-to-CT™ 试剂盒提供了简单快捷的细胞裂解制备方法。 | |
| 视频：Cells to CT™ 试剂盒展示 在这项展示中，Richard Fekete 博士使用 Cells-to-Cт™ 试剂盒在 10 分钟内制备了 96 份样品，并将此过程与传统离心柱的 RNA 提纯方法进行了对比。 |