Ambion™ RNA Century™-Plus Markers comprise a set of 7 in vitro–generated transcripts of 100, 200, 300, 400, 500, 750, and 1,000 bases. The Century-Plus Markers and Century™ Marker Templates are ideal for use in isotopic and nonisotopic ribonuclease protection assays and when single-stranded RNA size standards are needed for denaturing gel electrophoresis. 

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Century Marker Templates

The Century™ Marker Templates contain mixtures of linearized plasmids ready for use as templates in in vitro transcription reactions for synthesis of labeled RNA molecular size standards. When transcribed with T7 RNA polymerase, Century™ Marker Templates produce 5 RNA transcripts of 100, 200, 300, 400, and 500 bases. Century™-Plus Marker Templates produce 7 RNA transcripts of 100, 200, 300, 400, 500, 750, and 1,000 bases.

RNA Century-Plus Markers

 

 

Figure 1. RNA Century-Plus Markers (2 µg), along with an RNA transcript, were electrophoresed on an 8 M urea/5% polyacrylamide gel and stained with ethidium bromide.

Century Marker selection guide

DNA LaddersBase Pair (bp) Range
 RNA Century-Plus Markers 100–1,000 bases
 Century Marker Templates 100–500 bases
 Century-Plus Marker Templates 100–1,000 bases
Century Marker selection guide

仅供科研使用，不可用于诊断目的。

Stylesheet for Classic Wide Template adjustments