A wide range of products—flexibility for your research

Dynabeads magnetic beads are available for covalent coupling of ligands to the bead surface. One can choose beads of different sizes, with a hydrophobic or hydrophilic surface, and with a variety of surface chemistries. For optimal results, select the right bead based on ligand type (Table 1). Note that in most applications several Dynabeads products can be used. Depending on the magnetic bead type, the ligand can be directly linked to the bead surface or indirectly linked through a reactive intermediate. Protocols can take place in a single tube, with just a few handling steps.

How surface-activated magnetic beads immobilize a target ligand

The Dynabeads are pipetted into a vial and the ligand is added. Incubate the vial for covalent coupling of the ligand to the Dynabeads, add target containing sample, incubate again for affinity-binding of target. Use a magnetic stand or rack for magnetic separation, and remove supernatant. Wash and either boil in SDS for page or elute for pure target protein.

Figure 1. Antibodies or other ligands can be coupled onto the beads for affinity protein capture.

Table 1. Choose the right surface-activated Dynabeads for your ligand and target sample

LigandTargetDynabeads Tosyl-
activated		Dynabeads
Carboxylic
Acid 		Dynabeads AmineDynabeads Epoxy
AntibodyLow MW antigen1 or peptide dotdotdotdotdotdotdotdotdot
Protein or antibodydotdotdotdotdotdotdotdot
Protein complex
dotdot
dotdotdotdotdot
Organellesdotdot  dotdot2
Phage1dotdotdotdotdotdotdotdotdot
Virusdotdotdotdotdotdotdotdotdot
Bacteriadotdotdotdotdot  
Cells3dotdotdotdotdot dotdotdot
Antibody FragmentPhage1 or antibodydotdotdotdotdotdotdotdotdotdot
ProteinPhage or carbohydratedotdotdotdotdotdotdotdotdot
Nucleic aciddotdotdotdotdotdot
PeptidePhage1 or antibodydotdotdotdotdotdotdotdotdotdot
CarbohydrateAntibody dotdotdotdotdot
Low MV antigenAntibodydotdotdotdotdotdotdotdot
Nucleic acids, oligonucleotide, aptamer, PNANucleic acid binding proteins1dotdotdotdot4 
DNA1, RNA1 or PCR amplicons1 dotdotdotdot4 
EnzymeSubstrate or target for enzyme degradationdotdotdotdotdotdotdot
Organic chemistry derivatization, including introduction of new functional groups dotdotdotdotdotdotdotdotdot

dotdotdot Best product choice for listed ligand and target.
dotdot Alternative choice for listed ligand and target.
dot Can be used for listed ligand and target.
1 Dynabeads pre-coupled with streptavidin may be the best product choice.
2 Dynabeads M-450 Epoxy.
3 Dynabeads M-450 Epoxy are recommended for isolation, stimulation, and expansion of cells.
4 Bifunctional crosslinkers may be introduced to immobilize oligonucleotides onto the surface of Dynabeads Amine.

Surface-activated Dynabeads product list

Resources

仅供科研使用，不可用于诊断目的。