NovaBright Reporter Gene Assay Selection Guide

	NovaBright Beta-gal System	NovaBright Dual Beta-Gal & Luciferase System	NovaBright Luciferase System	NovaBright SEAP System
Reporter Enzyme(s)	Beta-galactosidase	Luciferase, beta-galactosidase	Luciferase	Secreted placental alkaline phosphatase
Key Attributes	Homogeneous assay does not require removal of culture medium	Detection of luciferase and beta-galactosidase from the same cell extract	Ideal for high-throughput screening	No cell lysis required
Substrate	Galacton-Star®, Gal-Star®, Galacton-Plus®	Luciferin, Galacton-Plus®	Luciferin	CSPD®
Duration of Light Emission	60–90 min	60–90 min	60–90 min	1–2 hr
Reagent Injection	Not required	Required	Not required	Not required
Special Features	Designed for assays on cells cultured in luminometer plates for high-throughput screening. Choice of reaction buffers for lysis of mammalian or yeast and mammalian cells.	Two assays from one cell extract enables better precision for normalizing transfection efficiency.	Linear signal from 50 fg to 100 ng, stable light emission from 10 minutes to 4-5 hours.	Cells remain viable; useful for stable transfectants, time course studies, etc.
	See all Beta-gal Kits	See all Dual Systems	See all Luciferases	See all SEAP Kits

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