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NucBlue Live reagent (Hoechst 33342 dye) stains the nuclei of all cells. NucBlue Live reagent is frequently used to distinguish condensed nuclei in apoptotic cells.
1. Culture cells in an appropriate medium and vessel for fluorescence microscopy. |
2. Add two drops of NucBlue Live ReadyProbes Reagent per milliliter of medium. |
3. Incubate for 20 minutes, protected from light. |
4. Image the cells. |
NucBlue | |
---|---|
Excitation/Emission (nm) | 360/460 |
Standard filter set | DAPI |
EVOS Light Cube | DAPI |
Storage conditions | Room temperature |
U2OS live-cell staining. Live U2OS cells were imaged 15 minutes following staining with NucBlue Live ReadyProbes Reagent.