Flow Cytometry Protocols

• Human CD & Other Cellular Antigens
• Mouse CD & Other Cellular Antigens
• Antibody Fixation Considerations
•  Flow Cytometry Staining Index
• Phospho Flow Cytometry Antibody Clone Buffer Selection Guide
• Intracellular Staining Quick Guides
• Intracellular Staining Buffer Selection Guide

•  Super Bright Staining Buffer protocol
• Cell Preparation for Flow Cytometry Protocols (Invitrogen eBioscience reagents)
• Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers (Invitrogen eBioscience reagents)
• Staining Cell Surface Targets for Flow Cytometry (Invitrogen eBioscience reagents)
• Staining Intracellular Antigens for Flow Cytometry (Invitrogen eBioscience reagents)
• OneComp and UltraComp Compensation Beads Protocols for Flow Cytometry (Invitrogen eBioscience reagents)

• Isolation of human peripheral blood mononuclear cells from whole blood
• Monocyte isolation from peripheral blood mononuclear cells
• NK cell isolation from peripheral blood mononuclear cells
• T cell isolation from peripheral blood mononuclear cells
• CD4⁺ T cell isolation from peripheral blood mononuclear cells
• CD8⁺ T cell isolation from peripheral blood mononuclear cells
• Regulatory T cell isolation from peripheral blood mononuclear cells
• T cell isolation from secondary lymphoid organs
• Immune cell counting using automated cell counters
• Mouse Bone Marrow Cell Isolation
• Mouse Spleen Cell Isolation Protocol
• Whole Blood Staining Protocol for Flow Cytometry Analysis

• In vitro differentiation of macrophages from monocytes via M-CSF
• T-cell activation via functional antibodies anti-CD3 and anti-CD28
• Immune cell activation for cytokine production via LPS
• Stimulation of cytokine production in immune cells
• T-cell activation via Concanavalin A
• T-cell activation with Dynabeads Human T-Activator CD3/CD28

• LIVE/DEAD Fixable Dead Cell Stains Protocol
  
• LIVE/DEAD Sperm Viability Kit Flow Cytometry Protocol
• SYTOX Dead Cell Stains Protocol
• Viability Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)

• LIVE/DEAD Violet Viability/Vitality Kit Protocol
• Annexin V Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
• Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)

• CellTrace CFSE Cell Proliferation Kit Protocol
• CellTrace Far Red Cell Proliferation Kit Protocol
• CellTrace Violet Cell Proliferation Kit Protocol
• BrdU and EdU Double Staining Protocol
• Click-iT EdU Protocol for Flow Cytometry
•  Click-iT Plus EdU Flow Cytometry Assay Kits
•  BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
• Staining Cells with eFluor Proliferation Dyes for Flow Cytometry (Invitrogen eBioscience reagents)

• Vybrant DyeCycle Violet Stain
• Vybrant DyeCycle Green and Orange Stains
• Vybrant DyeCycle Ruby Stain
•  FxCycle Violet Stain
•  FxCycle Violet Ready Flow Reagent
•  FxCycle PI/RNase Staining Solution
•  FxCycle Far Red Stain
•  CellEvent Senescence Green Flow Cytometry Assay Kit

Search the full list of cell analysis protocols