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NGS Template Preparation Support
Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- What is the Ion Torrent™ Ion Chef™ system?
- Where can I find the protocols for my Ion Torrent™ Ion OneTouch™ 2 kit?
- What is the calibration standard in the Ion PGM™ Hi-Q™ OneTouch™ 2 Kit?
- Where can I find the Qubit conversion factor for the Ion Sphere Quality Control Assay?
- How do I update my Ion OneTouch 2 firmware?
Troubleshooting
- How much oil and recovery solution is typically consumed during a normal Ion OneTouch 2 run and during a cleaning?
- What should I do if an "Over Pressure" error appears after I start an Ion OneTouch 2 run?
- What are these black precipitates in the recovery tube and waste of the Ion OneTouch System?
- What should I do if the centrifuge is still running even though the time remaining is 0:00 at the final spin of the Ion OneTouch 2?
Guides and Tools
General
- Application Note: The Ion PGM™ System, With 400-base Read Length Chemistry, Enables Routine High-Quality de novo Assembly of Small Genomes
- Application Note: Discover Variants in 46 Cancer Genes Using the Ion AmpliSeq™ Cancer Panel v1
- Application Note: Ion AmpliSeq™ Designer Provides Full Flexibility to Sequence Genes of your Choice
- Brochure: Ion AmpliSeq™ DNA Custom Panels
- Brochure: Ion AmpliSeq™ Comprehensive Cancer Panel
- Brochure: Ion AmpliSeq™ Inherited Disease Panel
- Brochure: Ion AmpliSeq™ Sample ID Panel
- Schematic: A History of Innovation in Genetic Analysis
Template Preparation and Sequencing Protocols
Ion Chef™ System with Ion S5™ System/Ion S5™XL System
- Quick Reference: Ion 520™ and Ion 530™ Kit - Chef
- Quick Reference: Ion™ 540 Kit - Chef
- User Guide: Ion 520™ and Ion 530™ Kit - Chef
- User Guide: Ion 540™ Kit - Chef
Ion™ Chef System with Ion™ PGM System
- Quick Reference: Ion PGM™ Hi-Q™ View Chef Kits
- Quick Reference: Ion PGM™ Hi-Q™ Chef Kit
- Quick Reference: Ion PGM™ IC 200 Kit
- User Guide: Ion PGM™ Hi-Q™ View Chef Kits
- User Guide: Ion PGM™ Hi-Q™ Chef Kit
- User Guide: Ion PGM™ IC 200 Kit
Ion Chef™ System with Ion Proton™ System
- Quick Reference: Ion PI™ Hi-Q™ Chef Kit
- User Guide: Ion PI™ Hi-Q™ Chef Kit
Ion Chef™ Software User Guide (use with Torrent Suite Software (TSS) v4.4 and greater)
- User Bulletin: Ion Chef™ Instrument v4.4 Software
Ion OneTouch™ 2 System with Ion S5™ System/Ion S5™XL System
- Quick Reference: Ion 520™ and Ion 530™ Kit for the Ion OneTouch 2 System
- Quick Reference: Ion 540™ Kit for the Ion OneTouch 2 System
- User Guide: Ion 520™ and Ion 530™ Kit for the Ion OneTouch 2 System
- User Guide: Ion 540™ Kit for the Ion OneTouch 2 System
Ion OneTouch™ 2 System with Ion Proton™ System
- Quick Reference: Ion PI™ Hi-Q™ OT2 200 Kit
- Quick Reference: Ion PI™ Template OT2 200 Kit v3
- Quick Reference: Ion PI™ Template OT2 200 Kit v2
- User Guide: Ion PI™ Hi-Q™ OT2 200 Kit
- User Guide: Ion PI™ Template OT2 200 Kit v3
- User Guide: Ion PI™ Template OT2 200 Kit v2
Ion OneTouch™ 2 System with Ion PGM™ System
- Quick Reference: Ion PGM™ Hi-Q™ View OT2 Kit
- Quick Reference: Ion PGM™ Hi-Q™ OT2 Kit
- Quick Reference: Ion PGM™ Template OT2 200 Kit
- Quick Reference: Ion PGM™ Checklist - Ion OneTouch™ 2 Template Preparation (200-base reads)
- Quick Reference: Ion PGM™ Checklist - Ion OneTouc™h ES Enrichment (200-base reads)
- User Guide: Ion PGM™ Hi-Q™ View OT2 Kit
- User Guide: Ion PGM™ Hi-Q™ OT2 Kit
- User Guide: Ion PGM™ Template OT2 200 Kit
Ion OneTouch™ 2 Isothermal Amplification Kit with Ion PGM™ System
- Quick Reference: Ion PGM™ Template IA 500 Kit
- User Guide: Ion PGM™ Template IA 500 Kit
Search our database for Product Documents: Manuals, Vector Maps, Citations & References
Search our database for Quality and Safety Documents: MSDS, COA
- Overview: Next-Generation Sequencing
- Overview: Ion Torrent Next-Generation Sequencing Applications
- Overview: Ion Torrent Next-Generation Sequencing Workflow
- Overview: Ion Torrent Next-Generation Sequencing Technology
- Overview: Sequencing Education
- Overview: Targeted Sequencing by Ion Torrent Next-Generation Sequencing
- Quote Request: Ion AmpliSeq Request a Quote
- Citations: Ion Torrent Next-Generation Sequencing Publications & Literature
- Selection: Choose Next-Generation Sequencing or Sanger Sequencing Solutions
- Video: Introduction to Ion Torrent Next-Generation Sequencing Technology
- Videos: Ion Torrent Next-Generation Sequencing Featured Researchers
- Workflow: Ion Torrent Next-Generation Sequencing Workflow
- Ion Academy: An exclusiveIon-authorized user educational group with live webinar session featuring the latest Ion Torrent information
- Browse our site for: Training, Events, and Webinars
- Ion AmpliSeq Designer: The Ion AmpliSeq Designer allows you to design your own AmpliSeq panel using the preloaded genomes or by uploading your own reference sequence.
- Browse our site for: Mobile and Desktop Apps
Custom Services
Ion Torrent Certified Service Provider Program
Browse our global network of validated service providers that helps enable researchers in small and medium-sized laboratories to get high-quality exome and transcriptome sequencing data generated on the Ion Proton System from small amounts of input material with very fast turnaround. We offer services including whole-exome sequencing, whole-transcriptome sequencing, and targeted transcriptome sequencing.
Ion AmpliSeq Custom DNA Panels
We can create and design customized Ion AmpliSeq Panels to fit your needs. Use our free online assay design tool that connects you directly to our assay design pipeline. Panels can be designed against a number of preloaded genomes, or for any other organism by uploading your own FASTA file reference sequence.
Ion AmpliSeq Community Panels
Leveraging the power of the Ion Community and Ion AmpliSeq technology, we have developed Ion AmpliSeq Community Panels. Designed with leading disease researchers, Ion AmpliSeq Community Panels provide the ability to selectively analyze gene content with the aim of transforming cancer and inherited disease research. Your disease research is greatly simplified with the community-wide availability of these panels. In addition, for maximum flexibility, you will be able to rapidly customize any Ion AmpliSeq Community Panel to advance clinical research to fit your own project requirements.
For Research Use Only. Not for use in diagnostic procedures.