Thank You | Thermo Fisher Scientific

このオンラインツールを使用して、メソッドをHPLCからUHPLCにメソッド移行できます。新しいグラジエントテーブルを算出し、場合によっては注入量や流速、分析時間を調整します。現在のメソッドを入力して、UHPLCを使用したときに節約できる時間や溶媒量、サンプル量を確認してください。

このツールはご自身の責任においてご使用ください。

Use at your own risk. Prepopulated data are estimates only. Thermo Fisher Scientific takes no responsibility for the accuracy of data, calculations or results.

Current Method

Current Column

Length (mm):

Diameter (mm):

Particle Size (µm):

UHPLC Method

Planned Column

Length (mm):

Diameter (mm):

Particle Size (µm):

Peak Details (Critical Pair)

Actual R_s: (Resolution Factor)

Peak Details (Critical Pair)

Predicted R_s Change Factor:

Predicted R_s:

Current Method Conditions

Flow (mL/min):

Injection Volume (µL):

Max Observed Pressure:

Pressure Units:

Number of Samples:

Data Collection Rate (Hz):

Gradient Delay Vol:

Gradient Delay Volume (µL):

Recommended Method Conditions

* Boost Factor:

* Use this factor to increase the flow rate of the fast LC method. Note: if factor other than 1 is used, the resolution calculation is disabled.

Flow (mL/min):

Injection Volume (µL):

Estimated Max Observed Pressure:

0 bar

Number of Samples:

Data Collection Rate (Hz):

0 Hz

Gradient Delay Volume (µL):

Optimal GDV (µL):

Adjust Flow:

Current Gradient Table

Step	Time (min)	%A
1		100.0
2
3
4
5
6
7
8
9
10
End Time:	0.000
Recommended Reconditioning Time: n.a.

Planned Gradient Table

Time (min)	%A	%B	%C	%D
0.00	100.0









End Time:	0.000
Recommended Reconditioning Time: n.a.

Sample usage savings

Current Usage	Planned Usage	Savings

Eluent usage and time savings

	Current Total	Planned Total	Savings	Throughput
Eluent Usage
Time

HPLC Method Transfer Calculator

Current Method

UHPLC Method

Current Gradient Table

Planned Gradient Table

Sample usage savings

Eluent usage and time savings

Total Time Savings

Total Eluent Savings

Total Sample Usage Savings