Modern biopharmaceutical and proteomic laboratories perpetually seek to improve the speed, sensitivity and reproducibility of protein analysis. To this end, significant advances have been made in chromatography and mass spectrometry instrumentation. However, in virtually all cases, the protein sample must be treated in some way before it can be processed by these sophisticated machines. In fact, sample preparation is a critical first step in the analytical process, allowing sensitive hardware to accommodate diverse proteins presented in highly variable matrices.

In this eBook, we will cover:

  • Sample preparation – a critical first step for protein characterization and quantitation
  • How to achieve simpler and faster digestion
  • Improving reproducibility
  • Greater sensitivity
  • Dramatic effects of buffers and additives on efficiency of tryptic digestion
  • Digest buffers for enhanced protein solubilization
  • Major improvements in sensitivity–“even for difficult proteins”
  • Enhanced reproducibility of data
  • Faster, simpler, more reproducible and sensitive SPE clean-up
  • A new approach to micro elution
Simpler, Faster and More Reproducible Protein Digestion

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