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フローサイトメトリーの教育ビデオおよび e-learning
フローサイトメトリーのトピックスに関する技術的および教育的なビデオ
これらのビデオ、チュートリアルおよび e-learning は、フローサイトメーターのほか、フローサイトメトリー研究応用およびテクニックに関する技術的および教育的情報を提供するように構成されています。
関連するビデオ
癌免疫療法および白血病におけるアコースティックサイトメトリーの役割
Josep Carreras Leukaemia Research Institute の Jordi Petriz, Ph.D. から、フローサイトメトリーを用いた免疫細胞の検出および解析について学びましょう。このビデオで、Petriz博士は血液癌の次世代の免疫療法を創出するためにフローサイトメトリーを使用することについて述べています。
マルチパラメーターフローサイトメトリーのためのパネルデザイン
フィールドアプリケーションサイエンティストの Carol Oxford といっしょにパネルをデザインしましょう。適切なコントロールと抗体のタイトレーションを行うことの重要性を学びましょう。蛍光スピルオーバーの重要性を理解し、蛍光スペクトルバランスを見つけましょう。
T 細胞キメラ抗原受容体（CAR-T）の複雑な薬理学
このウェビナーでは、カリフォルニア大学サンディエゴ校 Cell Therapy Translational Laboratory（CTTL）責任者である Charles Prussak（PharmD, PhD）が、第一世代の CAR-T 療法、CD19 標的CAR-T 療法を実施したときに最も多くみられる毒性、および血液腫瘍と悪性固形腫瘍の治療のために創出され臨床試験が行われている次世代 CAR-T 細胞について議論します。
フローサイトメトリー機器の最近の技術的進歩により、どの程度スループットが向上したでしょうか。
このウェビナーでは、Purdue University の J. Paul Robinson が、Invitrogen Attune NxT フローサイトメーターの進展に焦点を当て、Attune NxT システムのテクノロジーが現在および将来のどのアプリケーションに適しているかを概説します。
CYToMORE 2016—サイトメトリーの進展
第6回 Modena Advanced Course in Cytometry（CYToMORE）は、フローサイトメトリーの知識を高め新しいアプリケーションを学びたい研究者を対象としています。イタリアのSociety of Immunology, Clinical Immunology and Allergology（SIICA）と共同で開催され、この分野でトップクラスの国際的専門家を講師に迎え、2016 年に大きな成功を収めました。この発表では、フランス Pitié-Salpêtrière にある Faculté de Médecine Pierre et Marie Curie の Victor Appay が、彼が研究している「フローサイトメトリーを用いた免疫学的老化の評価」について議論します。
チュートリアル―フローサイトメトリー入門
このチュートリアルでは、フローサイトメーターの仕組み、フローサイトメトーターで散乱光および蛍光をどのように検出するか、また得られたデータをどのように解析するか、その基礎を見ていきます。
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| Type | Title and description | Categories |
|---|---|---|
| CYToMORE lecture video (2016) | ...and on the 8th day, God created cytometry Presented by J. Paul Robinson, Purdue University, USA | flow cytometers, flow cytometry, history, technology developments |
| CYToMORE lecture video (2016) | Assessing immunological aging using flow cytometry Presented by Victor Appay, Faculté de Médecine Pierre et Marie Curie, Pitié-Salpêtrière, France | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Asymptomatic but threatening: dissecting the consequences of cytomegalovirus infection Presented by Anis Larbi, Singapore Immunology Network – SigN, Singapore | flow cytometry assays, immunology |
| CYToMORE lecture video (2016) | Cancer stem cells and multi-drug resistance by flow cytometry Presented by Jordi Petriz, Josep Carreras Leukaemia Research Institute, IJC, Spain | cancer, flow cytometry, multidrug resistance (MDR), stem cell research |
| CYToMORE lecture video (2016) | Cytometric analysis of cytokines and transcription factors Presented by Hyun-Dong Chang, Deutsches Rheuma-Forschungszentrum, Berlin, Germany | cell signaling, flow cytometry, immunophenotyping |
| CYToMORE lecture video (2016) | Cytometry of immunity Presented by Andreas Radbruch, Deutsches Rheuma-Forschungszentrum Berlin, Germany | flow cytometry, immunology |
| CYToMORE lecture video (2016) | Cytomic analysis of oxidative stress Presented by J. Enrique O’Connor, University of Valencia, Spain | flow cytometry, oxidative stress |
| CYToMORE lecture video (2016) | High throughput and high content flow cytometry Presented by J. Paul Robinson, Purdue University, USA | Attune NxT, flow cytometer, high content flow cytometry, high throughput screening |
| CYToMORE lecture video (2016) | Heterogeneity of tumour-infiltrating leukocytes: implications for adoptive cell transfer immunotherapy Presented by Enrico Lugli, Humanitas Clinical and Research Center, Italy | flow cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Host-microbe interaction at mucosal surfaces Presented by Maria Rescigno, Istituto Europeo di Oncologia – IEO, Italy | flow cytometry, immunology, microbiology |
| CYToMORE lecture video (2016) | Mass cytometry in the phenomics era: an essential tool for gene/function studies Presented by Herve Luche, Centre d’Immunophénomique - CIPHE, France | mass cytometry, immunology, immunophenotyping |
| CYToMORE lecture video (2016) | Microenvironment cytometry Presented by Paul Smith, Cardiff University, UK | cancer, cell cycle, flow cytometry, tumor microenvironments, drug discovery |
| CYToMORE lecture video (2016) | Novel reagent solutions for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometry assays |
| CYToMORE lecture video (2016) | Rare cells analysis: from bench to clinics Presented by Andrea Cossarizza, University of Modena and Reggio Emilia, Italy | Attune NxT, flow cytometer, rare cell detection, rare event detection |
| CYToMORE lecture video (2016) | RNA flow cytometry Presented by Paul Wallace, Roswell Park Cancer Institute, USA | flow cytometry, in situ hybridization, RNA |
| CYToMORE lecture video (2016) | Software tutorial: what’s new Presented by Carmen Gondhalekar, Purdue University, USA | Attune NxT, flow cytometer, software |
| CYToMORE lecture video (2016) | Tuning acoustic forces for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometer |
| How-to video | Tutorial: Labeling a purified antibody Educational video on how to label your next antibody for imaging or fluorescence.Walk through the tutorial as Molecular Probes scientists demonstrate the protocol—including all the tips and tricks you'll want to know about for your next antibody labeling experiment.The video features Judie showing Curtis, a chemistry graduate student, how to label his monoclonal antibodies while saving time and maximizing yield. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| How-to video | How to site specifically label your antibody using SiteClick technology This video explains how to easily and site specifically label an antibody using an enzymatic and Click chemistry approach.This method can be applied to any intact IgG antibody and requires no antibody engineering or complex methodology.Unlike classic antibody conjugation techniques this breakthrough SiteClick technology allows for antibody conjugation with complete confidence that the label will not directly interfere with the antibody binding domain. | antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, Qdot, western detection |
| Technology video | Click-iT Plus EdU Proliferation Assay Measuring DNA synthesis is the most precise way to detect changes in cell proliferation.Image-based proliferation assays generate spatial and temporal results that can not be detected with other methods.This video describes and compares two of the most referenced image-based proliferation technologies. | cell proliferation, Click-iT, flow cytometry, fluorescence microscopy/fluorescence imaging |
| Technology video | The Two Worlds of Cell Separation | cell isolation, immunology |
| Tutorial video | Molecular Probes Tutorial Series—Analyzing Flow Cytometry Data | flow cytometer calibration, flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Anatomy of Fluorescence Spectra | absorbance, emission, excitation, spectra |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Flow Cytometry | flow cytometer calibration, multicolor flow cytometry |
| Tutorial video | Molecular Probes Tutorial Series—Introduction to Fluorescence | flow cytometry, fluorescence imaging |
| Tutorial video | Molecular Probes Tutorial Series—Overview of Filters and Light Sources | emission, excitation, filters, light cubes, light sources |
| Video (Meet the Inventor) | Greg Kaduchak (Engineering Director Flow Cytometry, Thermo Fisher Scientific) gives an overview of the design and capabilities of the acoustic-assisted cytometry that's at the core Attune NxT Flow Cytometer. | Attune NxT |
| Video (Meet the Inventor) | Jolene Bradford (Associate Director Flow Cytometry, Thermo Fisher Scientific) describes how the software of the Attune NxT Flow Cytometer helps users automate routine maintenance tasks, instrument performance alerts, and system cleaning. | Attune NxT |
| Video (Meet the Inventor) | Jolene Bradford (Associate Director Flow Cytometry, Thermo Fisher Scientific) speaks about how the fluidics system within the Attune NxT Flow Cytometer helps users minimize sample usage and avoid running buffer reservoirs dry. | Attune NxT |
| Video (Meet the Inventor) | Wesley Smith (Mechanical Engineer Flow Cytometry, Thermo Fisher Scientific) discusses the variety of laser configurations available on the Attune NxT Flow Cytometer, designed to support more advanced experiments and growing lab needs. | Attune NxT |
| Video (Meet the Inventor) | Michael Ward (Engineering Lead Flow Cytometry, Thermo Fisher Scientific) speaks about how the design of the Attune NxT Flow Cytometer offers smooth sample introduction, broadly variable volumetric flow rates, and robust performance for improved accuracy in cell analysis. | Attune NxT |
| Webinar | A practical approach to antibody labeling The growing number of fluorophores available makes labeling your own antibodies a tempting proposition.But with many antibody labeling solutions available, selecting the best option can be a daunting task.In this webinar we will:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF) |
| Webinar | An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis. | apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability |
| Webinar | An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis In this webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells.Changes introduced by apoptosis can be tested with numerous assays measuring membrane structure, mitochondrial function, metabolism, caspase activity, membrane integrity, and DNA fragmentation.We will also discuss dead cell identification using traditional impermeant nucleic acid dyes. | apoptosis, cell cycle, cell proliferation, multicolor flow cytometry, viability |
| Webinar | Analysis of surface antigens on exosomes using the Attune NxT Flow Cytometer Join Steve McClellan as he shares how he analyzes individual exosomes for the expression of a variety of surface markers using multi-color flow cytometry. | Attune NxT, multicolor flow cytometry |
| Webinar | Basics of flow cytometry, Part I: Gating and data analysis An introduction to fluorescence, this presentation covers the following topics: the principle of the flow cytometry platform, the basic components of a flow cytometer, how to interpret a dye excitation/emission spectrum, how data is displayed, basic gating demonstration, and common statistics and terminology used in flow cytometry. | flow cytometry data, flow cytometry set up, multicolor flow cytometry |
| Webinar | Basics of flow cytometry, Part II: Compensation This presentation provides an overview of basic fluorochromes used in flow cytometry.Topic includes: the principle of compensation, how to perform compensation, the types of controls recommended and their use, basic strategies for designing a flow experiment, and data presentation. | flow cytometry data, flow cytometry set up, multicolor flow cytometry |
| Webinar | Basics of multi color flow cytometry panel design With the proliferation of new fluorescent dyes, as well as instruments that can detect 18 or more parameters multicolor flow cytometry has become more popular and more accessible than ever.This webinar presented by Dr. Holden T. Maecker at Stanford University will discuss the caveats of good panel design, including:
| antibodies, compensation, multicolor flow cytometry, |
| Webinar | DNA content cell cycle analysis using flow cytometry Find out how careful acquisition and methodical preparation contribute to accurate and consistent DNA analysis.In this webinar we’ll discuss:
| cell counting, cell cycle, cell proliferation, flow cytometry |
| Webinar | Flow cytometry in microbiological research In recent years the application of flow cytometry in microbiological research has expanded from detection and quantification of organisms to more complex studies including analysis of host-microbe interactions and detailed spatial and temporal analysis of microbial metabolism in different environments.During this webinar we will discuss how the multi-parametric nature of flow cytometry can be applied to microbiology and the advantages of using this application over traditional microbiological methods. | microbiology, multicolor flow cytometry, viability |
| Webinar | How recent technological advances in flow cytometry instrumentation are enabling faster throughput This webinar presented by J. Paul Robinson at Purdue University focuses on advances in the Invitrogen Attune NxT Flow Cytometer and outline where the technology within the Attune NxT instrument fits in current and future applications. | Attune NxT, cell cycle, multicolor flow cytometry |
| Webinar | Learn to choose the right fluorophore when designing experiments The choice of fluorophore is one of the first important decisions to make in developing an experiment.Fluorophores are compounds that emit light at a specific wavelength when they have been excited at another, lower wavelength.Join our webinar and explore:
| antibodies, antibody labeling, flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Webinar | Making polychromatic flow cytometry easy Join Grace Chojnowski, Flow Cytometry and Imaging Facility Manager at QIMR Berghofer Medical Research Institute as she discusses how to make polychromatic flow cytometry easy after instrument standardization and verification for different fluorochromes on different flow cytometers and cell sorters. | Attune NxT, multicolor flow cytometry |
| Webinar | Multiparameter cell cycle analysis Join James W Jacobburger as he shares how to get better cell recovery and definition of cell cycle compartments.This webinar focuses on multiparametric cell cycle analysis of DNA and specific epitopes using a “washless” staining assay to minimize sample handling. | Attune NxT, multicolor flow cytometry |
| Webinar | The Complex Pharmacology of T-Cell CARs In this webinar, Charles Prussak, PharmD, PhD., Director of the Cell Therapy Translational Laboratory (CTTL), UCSD, discusses the generation CAR-T therapies, toxicities that are most commonly observed when employing CD-19 directed CAR-T therapies, and the next generation of CAR-T cells being created and clinically tested for the treatment of both hematological and solid tumor malignancies. | flow cytometry, immuno-oncology |
| Webinar | Panel Design for Multi-Parameter Flow Cytometry Join FAS Carol Oxford on panel design.Learn the value of including the correct controls and reagent titration.Understand the importance of fluorescence spillover and finding your fluorescence spectral balance. | multicolor flow cytometry, Attune NxT Flow Cytometer |
| Webinar | Identify Cancer Stem Cells in Primary Tumors and in Circulation using Flow Cytometry Join Bruno Sainz as he discusses the advancements in the detection of cancer stem cells (CSC) in primary tumors and in circulation using acoustic flow cytometry. | flow cytometry |
| Webinar | Analyzing your CRISPR gene editing efficiencies using flow cytometry In this webinar, Natasha Roark, scientist at Thermo Fisher Scientific, discusses CRISPR gene editing technology and its role in flow cytometry editing efficiencies. | flow cytometry |
| Webinar | Advancing immuno-oncology discovery and development with flow cytometry In this webinar, Chris Langsdorf, scientist at Thermo Fisher Scientific, discusses how flow cytometry can effectively be employed in research efforts aimed at advancing the discovery and development of various immunotherapy strategies. | flow cytometry |
| Webinars (ongoing) | Protein and cell analysis upcoming educational webinars See our upcoming webinars and see what is now available on demand in our virtual learning platform. | education |