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For the growth and expression of glial cells (normal and tumor) of astrocytic phenotype.
G-5 Supplement is a chemically defined, 100X concentrate of Bottenstein G-5 formulation (1). This Supplement is designed for the growth and expression of primary glial cells(astrocytes) or tumor cell lines of astrocytic phenotype(astrocytes and gliomas). It can be used for continuous cell lines. It can be used as a substitute for the G-2 Bottenstein formulation.
Catalog Number(s)
17503012| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Vitamins | |||
| Biotin | 244.0 | 100.0 | 0.40983605 |
| Proteins | |||
| Basic FGF | 0.5 | Infinity | |
| EGF | 1.0 | Infinity | |
| Human Transferrin | 5000.0 | Infinity | |
| Insulin | 500.0 | Infinity | |
| Other Components | |||
| Hydrocortisone | 362.47 | 0.36 | 9.931857E-4 |
| Selenite | 173.0 | 0.52 | 0.0030057803 |
1. Bottenstein, J.E. (1985) Cell Culture in the Neurosciences, Bottenstein, J.E. and Harvey, A.L., editors, p. 3, Plenum Press: New York and London.