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For the growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype.
N-2 supplement is a chemically defined, 100X concentrate of Bottenstein's N-2 formulation (1). This supplement is recommended for the growth and expression of neuroblastomas and for the survival and expression of post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). It can be used as a substitute for the N-1 Bottenstein formulation. N-2 medium appears to be selective for neuronal cell lines and does not support the growth of nonneuronal cell lines. (in Current Methods in Cellular Neurobiology: Volume 4, edited by Jeffery Barker, John Wiley & Sons, Inc.,1983.)
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Proteins | |||
| Human Transferrin (Holo) | 78500.0 | 10000.0 | 0.12738854 |
| Insulin Recombinant Full Chain | 5807.7 | 500.0 | 0.0860926 |
| Other Components | |||
| Progesterone | 314.47 | 0.63 | 0.0020033708 |
| Putrescine | 161.0 | 1611.0 | 10.006211 |
| Selenite | 173.0 | 0.52 | 0.0030057803 |
1. Bottenstein, J.E. (1985) Cell Culture in the Neurosciences, Bottenstein, J.E. and Harvey, A.L., editors, p. 3, Plenum Press: New York and London.