Technical Resources - Media Formulations

11965 - DMEM, high glucose

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.030.00.4
L-Arginine hydrochloride211.084.00.39810428
L-Cystine 2HCl313.063.00.20127796
L-Glutamine146.0584.04.0
L-Histidine hydrochloride-H2O210.042.00.2
L-Isoleucine131.0105.00.8015267
L-Leucine131.0105.00.8015267
L-Lysine hydrochloride183.0146.00.7978142
L-Methionine149.030.00.20134228
L-Phenylalanine165.066.00.4
L-Serine105.042.00.4
L-Threonine119.095.00.79831934
L-Tryptophan204.016.00.078431375
L-Tyrosine disodium salt dihydrate261.0104.00.39846742
L-Valine117.094.00.8034188
Vitamins
Choline chloride140.04.00.028571429
D-Calcium pantothenate477.04.00.008385744
Folic Acid441.04.00.009070295
Niacinamide122.04.00.032786883
Pyridoxine hydrochloride206.04.00.019417476
Riboflavin376.00.40.0010638298
Thiamine hydrochloride337.04.00.011869436
i-Inositol180.07.20.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)111.0200.01.8018018
Ferric Nitrate (Fe(NO3)3"9H2O)404.00.12.4752476E-4
Magnesium Sulfate (MgSO4) (anhyd.)120.097.670.8139166
Potassium Chloride (KCl)75.0400.05.3333335
Sodium Bicarbonate (NaHCO3)84.03700.044.04762
Sodium Chloride (NaCl)58.06400.0110.344826
Sodium Phosphate monobasic (NaH2PO4-H2O)138.0125.00.9057971
Other Components
D-Glucose (Dextrose)180.04500.025.0
Phenol Red376.415.00.039851222

参照

1. Dulbecco, R. and Freeman, G. (1959) Virology 8:396. 

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41965 - DMEM, high glucose
Cat No: 41965039 , 41965047 , 41965062 , 41965120