Alexa Fluor 568 excitation shown as dotted line and emission shown as solid orange histogram
Brightness iconLaser line iconCommon filter set iconExcitation iconEmission iconPhotostability iconInstrument platform icon
4568Texas Red579603(in buffer) 3
(in antifade) 4		microscopy; flow cytometry; fluorescence microplate reader

Invitrogen Alexa Fluor 568 dye is a bright, red-orange-fluorescent dye with excitation designed for use with the 568 nm laser line on the Ar-Kr mixed-gas laser and matches well with RFP filter sets. For stable signal generation in imaging and flow cytometry, Alexa Fluor 568 dye is pH-insensitive over a wide molar range. In addition, it is used in stochastic optical reconstruction microscopy (STORM)—as a reporter in dSTORM.

Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 568 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

We offer Alexa Fluor 568 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to allow you to generate your own antibody conjugates or probes.

Search Alexa Fluor 568 secondary antibodies Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)

Experimental data using Alexa Fluor 568

Flow cytometry analysis of human mononuclear cells using Zenon Alexa Fluor 568 Labeling Kit.  An antibody complex was prepared using the Zenon Alexa Fluor 568 Mouse IgG1 Labeling Kit and mouse anti-human CD4. Human mononuclear cells were stained with the antibody complex and analyzed by flow cytometry using a 532 nm Laser.

Microscopic view of neurons stained with red polar tracer and green dextran probe showing co-localization in yellow

Co-stain of neuron with Dextran and Hydrazide. Confocal image stack of a 10,000 MW Calcium Green dextran-labeled climbing fiber in a sagittal cerebellar slice, showing incoming axon and terminal arborization (in yellow). The Purkinje cell innervated by this fiber was labeled with Alexa Fluor 568 Hydrazide.

Microscopic view of skin fibroblasts with F-actin (green), alpha-tubulin (orange) and cdc-6 peptide (blue)

Three-color staining of muntjac cells with Alexa Fluor Phalloidin conjugate and Zenon Mouse Labeling Kit. Fixed and permeabilized muntjac skin fibroblasts were stained with Alexa Fluor 488 Phalloidin, alpha-Tubulin Monoclonal Antibody, and anti-Cdc6 Polyclonal Antibody. The alpha-Tubulin antibody was prelabeled with the Zenon Alexa Fluor 568 Mouse IgG1 Labeling Kit, and anti-Cdc6 antibody was prelabeled with Zenon Alexa Fluor 350 Mouse IgG1 Labeling Kit.

Additional resources

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