Brilliant Violet 711 excitation shown as dotted line and emission shown as solid red histogram
4405710/50, 690 LP406711(in buffer) 3
 flow cytometry

Invitrogen Brilliant Violet™ 711 (BV711) is a tandem dye excited by the 405 nm violet laser and emits at 711 nm. This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BV711 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Violet™ 711 primary antibodies

Experimental data using Brilliant Violet™ 711

Spectral signature of Brilliant Violet™ 711 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD8a Monoclonal Antibody, Brilliant Violet™ 711 were used for analysis.

 

Dot plot of Ki-67 BV711 vs CD45R (B220) FITC in unstimulated and stimulated cells

Intracellular staining of mouse splenocytes using Brilliant Violet™ 711. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody (SolA15), Brilliant Violet™ 711. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation.

 

Dot plot of samples stained with IgG1 control vs CD3-FITC and CD4-BV711 vs CD3-FITC

Cell surface staining of human peripheral blood cells using Brilliant Violet™ 711. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC and Mouse IgG1 kappa Isotype Control, Brilliant Violet™ 711 (left) or CD4 Monoclonal Antibody, Brilliant Violet™ 711 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

 

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.

Additional resources

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.