NovaFluor Yellow 610 excitation shown as dotted line and emission shown as solid yellow-red histogram
4561590/40551614(in buffer) 5
flow cytometry

Invitrogen NovaFluor Yellow 610 dye can be used to replace PE/Dazzle™ 594, BD Horizon™ PE-CF594, or PE-Texas Red dye using the 561 nm yellow laser line for excitation. In comparison to these PE tandem dyes, NovaFluor Yellow 610 dye shows much less cross-excitation and lower spectral spillover with the 488 nm blue laser line, allowing detection of an additional marker labeled with, for example, one of the NovaFluor Blue 610 dyes.

The macromolecule-based NovaFluor Yellow 610 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Yellow 610 primary antibodies

Benefits of NovaFluor Yellow 610 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Can serve as a replacement for PE-tandem dyes using the yellow-green laser, with far less cross-laser excitation and spectral spillover
  • Single PE-tandems can be replaced by NovaFluor Blue 610 and NovaFluor Yellow 610 dyes, to allow detection of an additional marker in a flow cytometry panel
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Yellow 610 dye

Spectral signature of NovaFluor Yellow 610 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 610 were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
 

Side Scatter plot with unstained and CD16 NovaFluor Yellow 610-stained normal human PBMCs

Staining of human peripheral blood cells with NovaFluor Yellow 610 dye conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD16 Monoclonal Antibody (3G8), NovaFluor Yellow 610 (right). Data were acquired in the YG3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
 

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