Brilliant Violet 650 excitation shown as dotted line and emission shown as solid red histogram
2405660/20, 630 LP407646(in buffer) 3
 flow cytometry

Invitrogen Brilliant Violet™ 650 (BV650) is a tandem dye excited by the 405 nm violet laser and emits at 650 nm. This dye has a low-to-medium relative brightness and can be used for cell surface, intracellular, and nuclear staining. BV650 may have some cross-laser excitation with the red laser.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BV650 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Violet™ 650 primary antibodies

Experimental data using Brilliant Violet™ 650

Spectral signature of Brilliant Violet™ 650 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet™ 650 were used for analysis.

 

Dot plot of TNF-alpha-BV650 vs CD4-FITC in unstimulated and stimulated cells

Intracellular staining of human peripheral blood cells using Brilliant Ultra Violet™ 650. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (right). Cells were then stained intracellularly, using Intracellular Fixation & Permeabilization Buffer Set, CD4 Monoclonal Antibody (RPA-T4), FITC and TNF alpha Monoclonal Antibody, Brilliant Violet™ 650. Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor™ 780.

 

Dot plot of samples stained with IgG2a control vs CD3e-FITC and CD4-BV650 vs CD3e-FITC

Cell surface staining of mouse splenocytes using Brilliant Violet™ 650. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Violet™ 650 (left) or 0.25 µg of CD4 Monoclonal Antibody (RM4-5), Brilliant Violet™ 650 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

 

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.

Additional resources

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.