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From exceptional cellular images to innovative imaging technology that helps provide fast results, our goal for over 40 years has been to collaborate with researchers to educate, inform and inspire so that you can tell the most important cell-stories through your research.
Bookmark and stop by often to read how we’re working to help bring your science vision to life.
Related content
- The EVOS Cell Imaging Systems Article Collection
- The High-Content Screening Platforms Article Collection
Past articles in the Imaging Insights series:
Click chemistry: Nobel Prize chemistry enables novel cell proliferation detection and site-specific antibody labeling
Click chemistry is a ground-breaking chemical method used to attach a substrate of interest to a biomolecule with unprecedented specificity. The creators of this technique (Sharpless, Meldal, and Bertozi) were awarded the 2022 Nobel Prize in Chemistry, as the methodology has already had a significant impact on research in several application areas.
Learn how Thermo Fisher Scientific has leveraged click chemistry in two unique ways: first, as a labelling mechanism for cell proliferation assays that offer increased capabilities compared to traditional methods, and second, as a highly efficient, site-specific antibody labelling technique for reliable and reproducible results.
Click chemistry is a ground-breaking chemical method used to attach a substrate of interest to a biomolecule with unprecedented specificity. The creators of this technique (Sharpless, Meldal, and Bertozi) were recently awarded the 2022 Nobel Prize in Chemistry as it already has had a significant impact on the research community in several application areas.
Thermo Fisher Scientific utilizes this Nobel-winning technology in two unique ways, first to transform cell proliferation assay capabilities and second, to offer a highly effective solution for site-specific antibody labelling.
Why is Click Chemistry useful to researchers?
Drug discovery and disease models, particularly for oncology research, are better suited to assess cell proliferation in model organisms rather than dissociated cell culture. However, due to the ubiquitous distribution of amines and thiols within cells and their presence in cell culture, in situ labeling of proteins and newly synthesized nucleic acids is often difficult. Click chemistry labeling technology overcomes this obstacle by employing bioorthogonal reaction partners that are not endogenous in cells, tissues, or model organisms to allow in vivo detection of proliferating cells.
How can Click Chemistry help measure cell proliferation for animal models?
Utilizing this innovative technology, Thermo Fisher Scientific features Click Chemistry in our Click-iT EdU (5-ethynyl-2’-deoxyuridine) and Click-iT Plus EdU Cell Proliferation Kits. These kits offer all the tools necessary for assessing in vivo proliferation and fluorescent EdU detection with a wide range of fluorophores to help meet the needs of researchers. When click chemistry is used in a cell proliferation assay such as Click-iT EdU, the product’s chemistry removes the need for DNA denaturation or harsh cell treatment, adversely affecting sample integrity, cell morphology, image quality, and the ability to multiplex fluorophores. This allows it to be given to animals with minimal toxicity, further, it opens the possibility of live cell/tissue proliferation detection now that DNA denaturation is no longer needed. Click-iT EdU Cell Proliferation Kits can be used for a variety of applications to learn more about these applications, visit our informational pages: fluorescence microscopy, high content screening and flow cytometry.
How does click chemistry help label antibodies?
SiteClick Antibody Labeling Kits use click chemistry for site selective labeling. This is achieved by enzymatic modification of the carbohydrate domain of the antibody to attach an azide to the Fc region of the antibody, preventing fluorophore attachment to the antigen binding sites of the antibody, which renders it ineffective. The enzymatic modification allows for mild reaction conditions that help preserve antibody binding and structure. These features of SiteClick labeling are critical for antibody applications requiring high specificity with reproducible labeling reactions and help ensure that antibody performance and function are preserved after conjugation.
Conclusion
The click chemistry used in our Click-iT EdU and SiteClick products allows for, specific, and gentle labeling of nucleic acids and proteins, which can open exciting possibilities for assaying proliferation in live or fixed samples and help increase the quality and consistency of antibody labelling respectively.
To learn more about all of the Thermo Fisher Scientific cellular imaging offerings, tools and resources, bookmark and explore the latest by visiting the cellular imaging resource center at thermofisher.com/cellularimaging.
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Cell painting: Cellular imaging artform helps colorful insights emerge
An emerging technology, cell painting brings cellular images to life by rendering them with brilliant colors and rich scientific detail. How does it work? As a morphological profiling assay, it uses up to six fluorescent dyes, imaged in five channels, to reveal cellular components or organelles. It can also extract hundreds to several thousand measurements from each cell, sensing subtle changes in biological phenotype based on changes in size, shape, texture, and fluorescence intensity.
Cell painting: Cellular imaging artform helps colorful insights emerge
What is cell painting?
An emerging technology, cell painting brings cellular images to life by rendering them with brilliant colors and rich scientific detail. How does it work? As a morphological profiling assay, it uses up to six fluorescent dyes, imaged in five channels, to reveal cellular components or organelles.1 It can also extract hundreds to several thousand measurements from each cell, sensing subtle changes in biological phenotype based on changes in size, shape, texture, and fluorescence intensity. 1
Originally developed for the pharmaceutical industry, cell painting has supported drug discovery assay development and multiparameter screening campaigns.1 It can reveal patterns that help researchers understand disease mechanisms and predict a drug’s activity or mechanism of action.2
Why is cell painting so powerful?
Conventional high-throughput screening applications evaluate only a few measurements for the purposes of scale and are limited in their ability to detect different phenotypic changes caused by compound exposure. With cell painting, single cell measurements allow data extraction and identification of rare, but many times relevant, subpopulations of cells. This helps identify the cells most sensitive to compound exposure relative to the overall population.
As a profiling technique as opposed to a screening technique, cell painting casts a wider net, providing an unbiased approach that lets us make discoveries unrestricted by what we think we’re going to find.1 It helps us identify hits (compounds that have binding activity to the target) to initial discovery screens and provides mechanistic insights that can’t be gained from traditional high-throughput assays. It may also offer greater efficiency, as a single experiment can result in findings related to different diseases or biological processes.1
We’re innovating cell painting at Thermo Fisher Scientific
The power of cell painting is evident, and as an innovator in cellular imaging, we’re committed to getting this important technique into the hands of more researchers. If you’re struggling to use cell morphological and mechanistic data to advance your imaging analysis, we encourage you to get painting—cell painting. And we can help.
We recently introduced the Image-iT Cell Painting Kit, a turnkey assay solution that simplifies cell painting workflow. The kit is made up of a collection of curated reagents provided in a pre-optimized, ready-to-use format that takes the hassle out of your staining protocols. When combined with a high-content imager, such as the CellInsight platform, these reagents can provide a phenotypical readout of cell status. Furthermore, customizations can be made on the cell painting assay to accommodate a different range of targets or to accommodate a different cell type.
Our high-content CX7 Pro LED and LZR platforms are powered by the cell-painting HCS Studio 5.0 bio-application software, which allows you to:
- Collect all five channels of data required by the cell painting assay while laser autofocusing
- Populate all image collection settings and analysis parameters
- Collect images and analyze your data in parallel for the fastest time to data available, simplifying your cell painting workflow and supercharging your data
With Thermo Fisher Scientific’s cell painting solutions, researchers can obtain striking cellular images that not only advance their scientific understanding, but also tell the story of their research in eye-catching, publication-ready detail. It’s a true innovation, and we’re excited to see where it will take cellular imaging—and how we can advance it even further to help meet your needs.
For more details on our high-content platforms, please visit thermofisher.com/HCS
Curious how cell painting can tell the story of your research? We’re here to help.
Learn more about cell painting and download our cell painting application note.
Bookmark and explore more tools, resources and the latest information on cellular imaging from Thermo Fisher Scientific: thermofisher.com/cellularimaging
- Bray MA, Singh S, Han H, et al. Cell Painting, a high-content image-based assay for morphological profiling using multiplexed fluorescent dyes. Nat Protoc. 2016;11(9):1757-1774. >
- Chandrasekaran SN, Ceulemans H, Boyd JD, et al. Image-based profiling for drug discovery: due for a machine-learning upgrade? Nat Rev Drug Discov. 2021;20:145–159.
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Cellular imaging reagents: what features do you need most to take your research to brilliant new levels?
Thermo Fisher Scientific has asked for researcher feedback and they told us: compatibility between reagents and instrument filter sets are the number one unmet need when using cell imaging products. Does that top your wish list too? At Thermo Fisher Scientific, the goal is to dig deeper, so we can provide you with comprehensive cellular imaging solutions that work for your particular needs.
Cellular imaging reagents: What features do you need to take your research to brilliant new levels?
Thermo Fisher Scientific has asked for researcher feedback and they told us: compatibility between reagents and instrument filter sets are the number one unmet need when using cell imaging products. Does that top your wish list too? At Thermo Fisher Scientific, the goal is to dig deeper, so we can provide you with comprehensive cellular imaging solutions that work for your particular needs.
Like any researcher, at Thermo Fisher Scientific our curiosity knows no bounds. And now, we’re putting our curious nature to work uncovering the needs of those looking for more in cellular imaging research tools. Why? So we can continue to develop and deliver leading-edge technology that meets your needs. Maybe you’re looking for simplicity, speed, sensitivity, specificity, or scalability…or a combination of all five. Maybe you need something else altogether—take a short survey and share your point-of-view here.
We have a 40-year history of cell imaging thought-leadership, seeking and questioning to fulfill the vision of researchers. We’re a leading resource that offers innovative imaging instrumentation platforms and software with compatible validated reagents and dyes for those platforms . Don’t just take our word for it, take a quick look at what we have to offer for your research:
- EVOS digital microscopy platforms lead the industry in ease-of-use without the need of a darkroom. Easy-to-learn, safe, and affordable, EVOS systems are designed to help eliminate the complexities of high-end microscopy without compromising performance. EVOS microscopes are exceptionally versatile and ideal for a broad range of imaging applications, delivering consistent and brilliant images. Which EVOS microscopy platform might be right for you?
Explore the selection guide - CellInsight high-content analysis platforms offer simultaneous analysis and data generation for unique speed; conducting imaging and analysis in parallel provides fastest time-to-data. Which high-content screening platform might be right for you?
Explore the selection guide - Pair your cell imaging platforms with our Invitrogen imaging reagents, recognized the world over as key components for publication-quality, compatible cellular images and analysis. We offer cellular structure and functional probes, labels, and counterstains, as well as tools for achieving high resolution, stunning cellular images. Alexa Fluor Plus secondary antibodies allow you to make low-abundance targets more visible, spend less time optimizing, and make your precious samples count. With more than 30,000 publications, and featuring the Alexa Fluor dye family, our entire Alexa Fluor secondary antibody portfolio has been successfully used over the last three decades of fluorescence imaging.
We’re experts in cellular imaging because we listen to and understand imaging researchers’ needs. And we have the comprehensive imaging portfolio of products and resources to prove it.
Bookmark and explore more tools, resources and the latest information on cellular imaging from Thermo Fisher Scientific: thermofisher.com/cellularimaging
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Imaging instruments that are efficient and easy to use
Is ease of use in your cellular imaging platform important to you?
Did you know that 69% of researchers don’t expect ease-of-use in their cellular imaging tools? That seems like a high number to us. Who wouldn’t look for simplicity in a platform? How about efficient operation? The EVOS family of digital microscopy systems offers intuitive software to help you capture high-resolution, publication-quality images in just a few clicks.
Looking for beautiful images in just a few simple clicks?
The central feature shared by all EVOS cell imaging systems is their remarkable image quality. In just a few clicks, you can capture clear, bright, publication-quality images (without the need for a darkroom) that can help you tell the story of your data. Here’s the proof: Explore the EVOS image gallery
Do you simply need a reliable and bright LED light source?
All EVOS cell imaging systems feature bright LED light sources that provide more than 50,000 hours of brilliant (yet adjustable) illumination that (unlike mercury arc lamps) remains constant with minimal degradation over time. Cost-efficient and environmentally friendly, EVOS LED light cubes are designed for precise control, minimal maintenance, and exceptional reliability. These interchangeable LED cubes enable precise control with plug-and-play capability. Here’s the proof: Learn more about the EVOS LED light cubes
Maybe it’s efficiency and safety that you seek?
EVOS microscopes are designed with scientists’ workspace and workflow needs in mind. The size, portability, and robustness of EVOS microscopes enable you to capture and view images when and where needed. A large, bright, high-definition monitor enables you to view your cells outside the confines of a darkroom and helps facilitate safe collaboration in nearly any space. Here’s the proof: Browse and compare EVOS models
We’re experts in cellular imaging because we have over 40 years of history of cell imaging innovation and we understand imaging researchers’ needs. We are here for you, because when it comes down to it, our vision is to help fulfill yours.
Bookmark and explore more tools, resources and the latest information on cellular imaging from Thermo Fisher Scientific: thermofisher.com/cellularimaging
1. The Life Science Dashboard. Fluorescent Imaging Dashboard (North America & Europe). Carlsbad, CA: Percepta Associates, Inc; 2013:13.
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Our wheels of innovation never stop turning when it comes to cellular imaging at Thermo Fisher Scientific
Through our market research, you have told us you look for innovation from an imaging supplier. After all, in a field where technology is always advancing, no one wants to be left behind. We know this because we listen to our customers and actively seek out feedback.
The wheels of innovation never stop turning when it comes to cellular imaging at Thermo Fisher Scientific
Why? Because for us, hearing from researchers helps us drive innovation for you. With 40 years dedicated to cellular imaging research, Thermo Fisher Scientific develops the advanced technology you need so you can spend more time focused on discovery and less time try to find the best tools for your research.
That said, we don’t just provide instruments, software, and reagents for imaging. We also provide the support and education researchers need to take full advantage of our tools and products—not to mention the inspiration to help open the door to new possibilities.
Not sure where to start with cellular imaging? We’ve compiled a resource center that provides you with knowledge based imaging information, inspiration, tips and tricks to help you with your research.
Are you neck deep in your experiments, but have hit a roadblock? Get back on track by taking a look at expert recommendations for commonly encountered problem scenarios.
Curious to see what’s possible with our cellular imaging products? Get inspired with a tour of our image and video galleries and discover the possibility of capturing beautiful, clear, bright, publication-quality images that help you tell the story of your data.
We take pride in supplying researchers with solutions for every step in your workflow. And it’s important that we understand what you’re looking for to meet your specific needs. The resource center is also a place where you can let us know what your priorities are—whether it’s an innovative platform, easy-to-use technology, or the expertise to help save time and accelerate your goals, you can let us know
Because when it comes down to it, our vision is to help fulfill yours.
Bookmark and explore more tools, resources and the latest information on cellular imaging from Thermo Fisher Scientific: thermofisher.com/cellularimaging
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