78420-Phosphatase-Inhibit-230

샘플 내 인단백질의 완전성을 보존합니다

Phosphatase 억제제 칵테일 및 정제에는 serine, threonine, tyrosine phosphatase를 포적으로 하는 화학물질이 포함되어 있습니다. Halt™ 액체 칵테일은 100 μL 일회용 또는 1, 1 x 5 및 10 mL 포장 사이즈로 이용할 수 있습니다. Pierce™ 정제는 10 mL 용적의 한 가지 사이즈로 다양한 용적 및 가격 필요에 맞출 수 있습니다.

귀하의 실험에 가장 적합한 형태를 선택하세요

 Halt Phosphatase Inhibitor CocktailPierce Phosphatase Inhibitor Tablet
Flexible addition based on sample volume?YesNo
Contains DMSOYesNo
Requires reconstitutionNo (100X)Yes
Protein assay compatibilityAllBCA (dilute sample 1:3)
Downstream compatibilityAllAll
PricingPremiumEconomical
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세포 및 조직 추출물 내 phosphatase에서 단백질 인산화를 보존합니다.

A12n04-Fig3
Thermo Scientific Pierce Phosphatase Inhibitors preserve protein phosphorylation from phosphatases in cell and tissue extracts. Relative levels of total and phosphorylated protein were determined following protein extraction in the absence (–) or presence (+) of phosphatase inhibitors for (A) AKT and PDGFR in serum-starved, PDGF-stimulated (100 ng/mL) NIH 3T3 cell extracts and (B) ERK1/2 in liver and spleen tissue extracts. C: The degree of inhibition for protein, acid and alkaline phosphatase activity was determined in mouse brain extract following treatment with Pierce Phosphatase Inhibitor Tablets or another commercially available phosphatase inhibitor tablet.

자료

For Research Use Only. Not for use in diagnostic procedures.