Brilliant Violet 480 excitation shown as dotted line and emission shown as solid blue histogram
3405525/40437478(in buffer) 3
 flow cytometry

Invitrogen Brilliant Violet™ 480 (BV480) is a tandem dye excited by the 405 nm violet laser and emits at 480 nm. This dye has a medium relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BV480 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Violet™ 480 primary antibodies

Experimental data using Brilliant Violet™ 480

Spectral signature of Brilliant Violet™ 480 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet™ 480 were used for analysis.

 

Dot plot of Ki-67 BV480 vs CD45R (B220) FITC in unstimulated and stimulated cells

Intracellular staining of mouse splenocytes using Brilliant Ultra Violet™ 480. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody (SolA15), Brilliant Violet™ 480. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation.

 

Dot plot of samples stained with IgG2a control vs CD4-FITC and CD8a-BV480 vs CD4-FITC

Cell surface staining of mouse splenocytes using Brilliant Violet™ 480. C57BL/6 mouse splenocytes were stained with CD4 Monoclonal Antibody, FITC and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Violet™ 480 (left) or 0.25 µg of CD8a Monoclonal Antibody, Brilliant Violet™ 480 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

 

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.

Additional resources

Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.