eFluor 506 excitation shown as dotted line and emission shown as solid blue histogram
1405510/50419508(in buffer) 1flow cytometry

The eFluor 506 organic dye is a violet-laser excitable fluorophore with an emission peak of 506 nm and is optimized for use in flow cytometry applications. With similar spectra characteristics to AmCyan and Horizon V500 dyes, conjugates of eFluor 506 can be easily added to your multicolor panels without change in instrument configuration for use.

We offer eFluor 506 dye conjugated to primary antibodies for use in flow cytometry.

Search eFluor 506 primary antibodies

  • Direct alternative for AmCyan and Horizon V500 fluorophores
  • Similar or better fluorescence intensity than other 500 nm-emitting organic dyes
  • When combined with all five Super Bright dye antibody conjugates, can provide 6 detection channels of the violet laser
Flow histogram comparing eFluor 506 dye to Horizon V500 dye

Comparison of fluorescence intensity of eFluor 506 dye with Horizon V500. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 506 (purple line) or Horizon V500 (blue histogram), using the same concentration of antibody.

Flow histogram comparing eFluor 506 dye before and after fixation

Stability of eFluor 506 dye. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 506 and either left unfixed (blue line), or were fixed for 30 minutes in Foxp3/Transcription Factor Buffer Set (purple line) or 30 minutes in IC Fixation Buffer followed by methanol (orange line).


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Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.