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3 | 561 | 670/14 | 550 | 664 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Yellow 660 dye has been optimized for flow cytometers with spectral capabilities. Phiton technology provides highly stable fluorescence when stored at 4°C. NovaFluor Yellow 660 dye can be used to replace PE-tandems such as PE-Cy5 or PE-Fire™ 640 dye, with much less cross-excitation and fluorescent spillover. Replacement of PE-tandems with NovaFluor Yellow 660 dye, which is primarily excited by the yellow-green laser, opens a detector, allowing for an additional marker to be detected off of the blue laser line using NovaFluor Blue 660 dye.
Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Spectral signature of NovaFluor Yellow 660 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 660 were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Staining of human peripheral blood cells with NovaFluor Yellow 660 dye conjugated antibodyNormal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD3 Monoclonal Antibody (UCHT1), NovaFluor Yellow 660 (right). Data were acquired in the YG4 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.