NovaFluor Yellow 700 excitation shown as dotted line and emission shown as solid red histogram
3561695/40551701(in buffer) 5
flow cytometry

Invitrogen NovaFluor Yellow 700 dye can be used as an alternative to PE-Cyanine 5.5, with far less cross-laser excitation and spectral spillover. NovaFluor Yellow 690 and NovaFluor Yellow 700 dyes cannot be used together with conventional flow cytometry, but their distinct spectral signature allows them to be successfully unmixed using a spectral flow cytometer.

The macromolecule-based NovaFluor Yellow 700 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Yellow 700 primary antibodies

Benefits of NovaFluor Yellow 700 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Can serve as a replacement for PE-Cy5.5, with far less cross-laser excitation and spectral spillover
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Yellow 700 dye

Spectral signature of NovaFluor Yellow 700 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 700 were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
 

Side Scatter plot with unstained and CD16 NovaFluor Yellow 700-stained normal human PBMCs

Staining of human peripheral blood cells with NovaFluor Yellow 700 dye conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD16 Monoclonal Antibody (3G8), NovaFluor Yellow 700 (right). Data were acquired in the YG7 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.

Additional resources

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