Invitrogen

CD4 Monoclonal Antibody (OKT4 (OKT-4)), PE-Cyanine7, eBioscience™

FIGURE: 1 / 27

CD4 Antibody (25-0048-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4732-81) (left) or Anti-Human CD4 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

25-0048-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 27 publications 27 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT4 (OKT-4)

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573328

Product Specific Information

Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human blood peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Distinct SARS-CoV-2 specific NLRP3 and IL-1β responses in T cells of aging patients during acute COVID-19 infection.

Frontiers in immunology

Mahalingam SS,Jayaraman S,Arunkumar A,Dudley HM,Anthony DD,Shive CL,Jacobson JM,Pandiyan P

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Estrogen Protects against Renal Ischemia-Reperfusion Injury by Regulating Th17/Treg Cell Immune Balance.

Disease markers

Zhang Y,Chang Y,Han Z,Ma K,Zeng X,Li L

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

The neutrophil antimicrobial peptide cathelicidin promotes Th17 differentiation.

Nature communications

Minns D,Smith KJ,Alessandrini V,Hardisty G,Melrose L,Jackson-Jones L,MacDonald AS,Davidson DJ,Gwyer Findlay E

25-0048-42 was used in Flow cytometry/Cell sorting to investigate if cathelicidin is a powerful Th17 potentiator which enhances aryl hydrocarbon receptor (AHR) and RORγt expression, in a TGF-β1-dependent manner.

Wed Feb 24 00:00:00 EST 2021

Small-molecule antagonist of VLA-4 (GW559090) attenuated neuro-inflammation by targeting Th17 cell trafficking across the blood-retinal barrier in experimental autoimmune uveitis.

Journal of neuroinflammation

Chen YH,Eskandarpour M,Zhang X,Galatowicz G,Greenwood J,Lightman S,Calder V

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Thu Feb 18 00:00:00 EST 2021

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses.

Cancers

Beltrán Hernández I,Angelier ML,Del Buono D'Ondes T,Di Maggio A,Yu Y,Oliveira S

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

Selective Killing of Activated T Cells by 5-Aminolevulinic Acid Mediated Photodynamic Effect: Potential Improvement of Extracorporeal Photopheresis.

Cancers

Darvekar S,Juzenas P,Oksvold M,Kleinauskas A,Holien T,Christensen E,Stokke T,Sioud M,Peng Q

25-0048-42 was used in Flow Cytometry to explore the possibility of targeting activated human blood T cells ex vivo, using the concept of photodynamic therapy (PDT) with 5-aminolevulinic acid (ALA), a precursor of an endogenously synthesized photosensitizer protoporphyrin IX (PpIX) in combination with blue light.

Thu Feb 06 00:00:00 EST 2020

Expression of CD20 after viral reactivation renders HIV-reservoir cells susceptible to Rituximab.

Nature communications

Serra-Peinado C,Grau-Expósito J,Luque-Ballesteros L,Astorga-Gamaza A,Navarro J,Gallego-Rodriguez J,Martin M,Curran A,Burgos J,Ribera E,Raventós B,Willekens R,Torrella A,Planas B,Badía R,Garcia F,Castellví J,Genescà M,Falcó V,Buzon MJ

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Fri Aug 16 00:00:00 EDT 2019

Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding.

Scientific reports

Jarrell JA,Twite AA,Lau KHWJ,Kashani MN,Lievano AA,Acevedo J,Priest C,Nieva J,Gottlieb D,Pawell RS

25-0048 was used in Flow cytometry/Cell sorting to show microfluidic vortex shedding mediates intracellular delivery of mRNA into primary human T lymphocytes.

Fri Mar 01 00:00:00 EST 2019

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

Nature communications

Hsieh WC,Hsu TS,Chang YJ,Lai MZ

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

Complement receptor CD46 co-stimulates optimal human CD8⁺ T cell effector function via fatty acid metabolism.

Nature communications

Arbore G,West EE,Rahman J,Le Friec G,Niyonzima N,Pirooznia M,Tunc I,Pavlidis P,Powell N,Li Y,Liu P,Servais A,Couzi L,Fremeaux-Bacchi V,Placais L,Ferraro A,Walsh PR,Kavanagh D,Afzali B,Lavender P,Lachmann HJ,Kemper C

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Correlation of APRIL with production of inflammatory cytokines during acute malaria in the Brazilian Amazon.

Immunity, inflammation and disease

Pinna RA,Dos Santos AC,Perce-da-Silva DS,da Silva LA,da Silva RNR,Alves MR,Santos F,de Oliveira Ferreira J,Lima-Junior JC,Villa-Verde DM,De Luca PM,Carvalho-Pinto CE,Banic DM

25-0048 was used in Flow cytometry/Cell sorting to study the effect of A proliferation-inducing ligand and B cell activation factor plasma concentrations on the inflammatory responses associated with malaria infection.

Fri Jun 01 00:00:00 EDT 2018

Dexamethasone and Monophosphoryl Lipid A Induce a Distinctive Profile on Monocyte-Derived Dendritic Cells through Transcriptional Modulation of Genes Associated With Essential Processes of the Immune Response.

Frontiers in immunology

García-González PA,Schinnerling K,Sepúlveda-Gutiérrez A,Maggi J,Mehdi AM,Nel HJ,Pesce B,Larrondo ML,Aravena O,Molina MC,Catalán D,Thomas R,Verdugo RA,Aguillón JC

25-0048 was used in Flow cytometry/Cell sorting to study the transcriptional profile of monocyte-derived dendritic cells from healthy subjects to identify molecular regulators and pathways associated with the induction of tolerogenic properties in tolerogenic dendritic cells.

Wed Nov 20 00:00:00 EST 2019

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

25-0048 was used in Flow cytometry/Cell sorting to reveal that TIGIT+CD4+T cells provide a supportive microenvironment for CLL cells.

Sat Jan 29 00:00:00 EST 2022

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.

AIDS research and human retroviruses

Quintremil S,Alberti C,Rivera M,Medina F,Puente J,Cartier L,Ramírez E,Tanaka Y,Valenzuela MA

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.

Scientific reports

Muller L,Mitsuhashi M,Simms P,Gooding WE,Whiteside TL

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2016

The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals.

Journal of virology

Veillette M,Coutu M,Richard J,Batraville LA,Dagher O,Bernard N,Tremblay C,Kaufmann DE,Roger M,Finzi A

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

Journal of immunology (Baltimore, Md. : 1950)

Wertheimer AM,Bennett MS,Park B,Uhrlaub JL,Martinez C,Pulko V,Currier NL,Nikolich-Žugich D,Kaye J,Nikolich-Žugich J

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.

Blood

Achour L,Scott MG,Shirvani H,Thuret A,Bismuth G,Labbé-Jullié C,Marullo S

Published figure using CD4 monoclonal antibody (Product # 25-0048-42) in Flow Cytometry

Thu Feb 26 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

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CD4 Monoclonal Antibody (OKT4 (OKT-4)), PE-Cyanine7, eBioscience™

CD4 Antibody (25-0048-42) in Flow

Product Details

25-0048-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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