Invitrogen

CD4 Monoclonal Antibody (OKT4 (OKT-4)), APC-eFluor™ 780, eBioscience™

FIGURE: 1 / 27

CD4 Antibody (47-0048-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0088-42) and Mouse IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4732-80) (left) or Anti-Human CD4 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

47-0048-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT4 (OKT-4)

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573938

Product Specific Information

Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Distinct SARS-CoV-2 specific NLRP3 and IL-1β responses in T cells of aging patients during acute COVID-19 infection.

Frontiers in immunology

Mahalingam SS,Jayaraman S,Arunkumar A,Dudley HM,Anthony DD,Shive CL,Jacobson JM,Pandiyan P

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children.

Nature immunology

Xu Q,Milanez-Almeida P,Martins AJ,Radtke AJ,Hoehn KB,Oguz C,Chen J,Liu C,Tang J,Grubbs G,Stein S,Ramelli S,Kabat J,Behzadpour H,Karkanitsa M,Spathies J,Kalish H,Kardava L,Kirby M,Cheung F,Preite S,Duncker PC,Kitakule MM,Romero N,Preciado D,Gitman L,Koroleva G,Smith G,Shaffer A,McBain IT,McGuire PJ,Pittaluga S,Germain RN,Apps R,Schwartz DM,Sadtler K,Moir S,Chertow DS,Kleinstein SH,Khurana S,Tsang JS,Mudd P,Schwartzberg PL,Manthiram K

47-0048-42 was used in Flow cytometry/Cell sorting to provide evidence for persistent tissue-specific immunity to SARS-CoV-2 in the upper respiratory tract of children after infection.

Sun Jan 01 00:00:00 EST 2023

Logic-gated antibody pairs that selectively act on cells co-expressing two antigens.

Nature biotechnology

Oostindie SC,Rinaldi DA,Zom GG,Wester MJ,Paulet D,Al-Tamimi K,van der Meijden E,Scheick JR,Wilpshaar T,de Jong B,Hoff-van den Broek M,Grattan RM,Oosterhoff JJ,Vignau J,Verploegen S,Boross P,Beurskens FJ,Lidke DS,Schuurman J,de Jong RN

47-0048-42 was used in Flow cytometry/Cell sorting to show that HexElect® allows selective antibody activity on target cells expressing unique, potentially unexplored combinations of surface antigens.

Sat Oct 01 00:00:00 EDT 2022

Estrogen Protects against Renal Ischemia-Reperfusion Injury by Regulating Th17/Treg Cell Immune Balance.

Disease markers

Zhang Y,Chang Y,Han Z,Ma K,Zeng X,Li L

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Tue Oct 18 00:00:00 EDT 2022

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Small-molecule antagonist of VLA-4 (GW559090) attenuated neuro-inflammation by targeting Th17 cell trafficking across the blood-retinal barrier in experimental autoimmune uveitis.

Journal of neuroinflammation

Chen YH,Eskandarpour M,Zhang X,Galatowicz G,Greenwood J,Lightman S,Calder V

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Thu Feb 18 00:00:00 EST 2021

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses.

Cancers

Beltrán Hernández I,Angelier ML,Del Buono D'Ondes T,Di Maggio A,Yu Y,Oliveira S

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Wed Apr 15 00:00:00 EDT 2020

Expression of CD20 after viral reactivation renders HIV-reservoir cells susceptible to Rituximab.

Nature communications

Serra-Peinado C,Grau-Expósito J,Luque-Ballesteros L,Astorga-Gamaza A,Navarro J,Gallego-Rodriguez J,Martin M,Curran A,Burgos J,Ribera E,Raventós B,Willekens R,Torrella A,Planas B,Badía R,Garcia F,Castellví J,Genescà M,Falcó V,Buzon MJ

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Fri Aug 16 00:00:00 EDT 2019

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

Nature communications

Hsieh WC,Hsu TS,Chang YJ,Lai MZ

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

Complement receptor CD46 co-stimulates optimal human CD8⁺ T cell effector function via fatty acid metabolism.

Nature communications

Arbore G,West EE,Rahman J,Le Friec G,Niyonzima N,Pirooznia M,Tunc I,Pavlidis P,Powell N,Li Y,Liu P,Servais A,Couzi L,Fremeaux-Bacchi V,Placais L,Ferraro A,Walsh PR,Kavanagh D,Afzali B,Lavender P,Lachmann HJ,Kemper C

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Wed Oct 10 00:00:00 EDT 2018

Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis.

Atherosclerosis

Lebedeva A,Vorobyeva D,Vagida M,Ivanova O,Felker E,Fitzgerald W,Danilova N,Gontarenko V,Shpektor A,Vasilieva E,Margolis L

47-0048 was used in Flow cytometry/Cell sorting to establish a new ex vivo model of human atherosclerotic plaques that preserves the main cell types in plaques and the extracellular components in the context of native cytoarchitecture.

Fri Dec 01 00:00:00 EST 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.

Stem cell reports

Li Y,Brauer PM,Singh J,Xhiku S,Yoganathan K,Zúñiga-Pflücker JC,Anderson MK

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Tue Sep 12 00:00:00 EDT 2017

PD-L1 expression and prognostic impact in glioblastoma.

Neuro-oncology

Nduom EK,Wei J,Yaghi NK,Huang N,Kong LY,Gabrusiewicz K,Ling X,Zhou S,Ivan C,Chen JQ,Burks JK,Fuller GN,Calin GA,Conrad CA,Creasy C,Ritthipichai K,Radvanyi L,Heimberger AB

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Tax and Semaphorin 4D Released from Lymphocytes Infected with Human Lymphotropic Virus Type 1 and Their Effect on Neurite Growth.

AIDS research and human retroviruses

Quintremil S,Alberti C,Rivera M,Medina F,Puente J,Cartier L,Ramírez E,Tanaka Y,Valenzuela MA

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

Tumor-derived exosomes regulate expression of immune function-related genes in human T cell subsets.

Scientific reports

Muller L,Mitsuhashi M,Simms P,Gooding WE,Whiteside TL

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2016

The HIV-1 gp120 CD4-bound conformation is preferentially targeted by antibody-dependent cellular cytotoxicity-mediating antibodies in sera from HIV-1-infected individuals.

Journal of virology

Veillette M,Coutu M,Richard J,Batraville LA,Dagher O,Bernard N,Tremblay C,Kaufmann DE,Roger M,Finzi A

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

Journal of immunology (Baltimore, Md. : 1950)

Wertheimer AM,Bennett MS,Park B,Uhrlaub JL,Martinez C,Pulko V,Currier NL,Nikolich-Žugich D,Kaye J,Nikolich-Žugich J

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Sat Mar 01 00:00:00 EST 2014

CD4-CCR5 interaction in intracellular compartments contributes to receptor expression at the cell surface.

Blood

Achour L,Scott MG,Shirvani H,Thuret A,Bismuth G,Labbé-Jullié C,Marullo S

Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry

Thu Feb 26 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (OKT4 (OKT-4)), APC-eFluor™ 780, eBioscience™

CD4 Antibody (47-0048-42) in Flow

Product Details

47-0048-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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