Invitrogen

CD4 Monoclonal Antibody (OKT4 (OKT-4)), eFluor™ 450, eBioscience™

FIGURE: 1 / 1

CD4 Antibody (48-0048-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0088-42) and Mouse IgG2b K Isotype Control eFluor® 450 (Product # 48-4732-82) (left) or Anti-Human CD4 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

48-0048-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 20 publications 20 publications

Product Specifications

Species Reactivity

Human

Published species

Cynomolgus monkey, Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT4 (OKT-4)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016674

Product Specific Information

Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

NRF2/Itaconate Axis Regulates Metabolism and Inflammatory Properties of T Cells in Children with JIA.

Antioxidants (Basel, Switzerland)

Rajendiran A,Subramanyam SH,Klemm P,Jankowski V,van Loosdregt J,Vastert B,Vollbach K,Wagner N,Tenbrock K,Ohl K

48-0048-42 was used in Flow cytometry/Cell sorting to investigate how this distinct environment regulates T cell responses within the inflamed joints of patients with childhood rheumatism (JIA) by analyzing the behavior of NRF2-the key regulator of the anti-oxidative stress response-and its signaling pathways.

Thu Dec 08 00:00:00 EST 2022

Combining Immunocytokine and Ex Vivo Activated NK Cells as a Platform for Enhancing Graft-Versus-Tumor Effects Against GD2⁺ Murine Neuroblastoma.

Frontiers in immunology

Bates PD,Rakhmilevich AL,Cho MM,Bouchlaka MN,Rao SL,Hales JM,Orentas RJ,Fry TJ,Gilles SD,Sondel PM,Capitini CM

48-0048-42 was used in Flow cytometry/Cell sorting to determine if allogeneic hematopoietic stem cell transplantation could serve as a platform for inducing a graft-versus-tumor effect against neuroblastoma with combination immunocytokine and NK cells in a murine model.

Wed Oct 20 00:00:00 EDT 2021

Complement C1s and C4d as Prognostic Biomarkers in Renal Cancer: Emergence of Noncanonical Functions of C1s.

Cancer immunology research

Daugan MV,Revel M,Russick J,Dragon-Durey MA,Gaboriaud C,Robe-Rybkine T,Poillerat V,Grunenwald A,Lacroix G,Bougouin A,Meylan M,Verkarre V,Oudard SM,Mejean A,Vano YA,Perkins G,Validire P,Cathelineau X,Sanchez-Salas R,Damotte D,Fremeaux-Bacchi V,Cremer I,Sautès-Fridman C,Fridman WH,Roumenina LT

48-0048-42 was used in Flow cytometry/Cell sorting to find that complement activation, as reflected by high plasma C4d or as C4d deposits at the tumor site, was associated with poor prognosis in two cohorts of patients with ccRCC.

Sun Aug 01 00:00:00 EDT 2021

Neutralizing Antibodies Protect against Oral Transmission of Lymphocryptovirus.

Cell reports. Medicine

Singh S,Homad LJ,Akins NR,Stoffers CM,Lackhar S,Malhi H,Wan YH,Rawlings DJ,McGuire AT

48-0048 was used in Flow cytometry/Cell sorting to indicate that a vaccine capable of eliciting adequate titers of neutralizing antibodies targeting the AMMO1 epitope may protect against EBV acquisition and are therefore highly relevant to the design of an effective EBV vaccine.

Tue Jun 23 00:00:00 EDT 2020

Enhanced Anti-lymphoma Activity of CAR19-iNKT Cells Underpinned by Dual CD19 and CD1d Targeting.

Cancer cell

Rotolo A,Caputo VS,Holubova M,Baxan N,Dubois O,Chaudhry MS,Xiao X,Goudevenou K,Pitcher DS,Petevi K,Kachramanoglou C,Iles S,Naresh K,Maher J,Karadimitris A

48-0048 was used in Flow cytometry/Cell sorting to investigate whether chimeric antigen receptor anti-CD19-engineering of the CD1d-restricted invariant natural killer T cells would result in enhanced anti-lymphoma activity.

Mon Oct 08 00:00:00 EDT 2018

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

48-0048 was used in Flow cytometry/Cell sorting to reveal that TIGIT+CD4+T cells provide a supportive microenvironment for CLL cells.

Sat Jan 29 00:00:00 EST 2022

IL-21 and probiotic therapy improve Th17 frequencies, microbial translocation, and microbiome in ARV-treated, SIV-infected macaques.

Mucosal immunology

Ortiz AM,Klase ZA,DiNapoli SR,Vujkovic-Cvijin I,Carmack K,Perkins MR,Calantone N,Vinton CL,Riddick NE,Gallagher J,Klatt NR,McCune JM,Estes JD,Paiardini M,Brenchley JM

48-0048 was used in Flow cytometry/Cell sorting to investigate methods to promote Th17 cell recovery in individuals infected with HIV, showing that IL-21 and probiotic therapy improve Th17 frequencies, microbial translocation, and microbiome in antiretroviral-treated, SIV-infected macaques.

Tue Mar 01 00:00:00 EST 2016

Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection.

Journal of immunology (Baltimore, Md. : 1950)

Goodier MR,Rodriguez-Galan A,Lusa C,Nielsen CM,Darboe A,Moldoveanu AL,White MJ,Behrens R,Riley EM

48-0048 was used in Flow cytometry/Cell sorting to examine the effect of seasonal influenza vaccination on natural killer cell function and phenotype in previously unvaccinated individuals.

Fri Jul 01 00:00:00 EDT 2016

A Comprehensive Analysis of the Impact of HIV on HCV Immune Responses and Its Association with Liver Disease Progression in a Unique Plasma Donor Cohort.

PloS one

Zhang YH,Zhao Y,Rajapaksa US,Lawrence TM,Peng YC,Liu J,Xu K,Hu K,Qin L,Liu N,Sun H,Yan HP,Repapi E,Rowland-Jones S,Thimme R,McKeating JA,Dong T

48-0048 was used in Flow cytometry/Cell sorting to highlights the impact of HIV co-infection on HCV-specific CD4+ T-cell responses.

Mon Jul 24 00:00:00 EDT 2017

T-helper 17 cell polarization in pulmonary arterial hypertension.

Chest

Hautefort A,Girerd B,Montani D,Cohen-Kaminsky S,Price L,Lambrecht BN,Humbert M,Perros F

48-0048 was used in Flow cytometry/Cell sorting to study T helper 17 cell immune polarization in patients with pulmonary arterial hypertension.

Mon Jun 01 00:00:00 EDT 2015

A role for CCL28-CCR3 in T-cell homing to the human upper airway mucosa.

Mucosal immunology

Danilova E,Skrindo I,Gran E,Hales BJ,Smith WA,Jahnsen J,Johansen FE,Jahnsen FL,Baekkevold ES

48-0048 was used in Flow cytometry/Cell sorting to investigate how CCL28-CCR3 interactions are involved in the homeostatic trafficking of CD4(+) T cells to the upper airways.

Thu Jan 01 00:00:00 EST 2015

Durable complete responses off all treatment in patients with metastatic malignant melanoma after sequential immunotherapy followed by a finite course of BRAF inhibitor therapy.

Cancer biology & therapy

Wyluda EJ,Cheng J,Schell TD,Haley JS,Mallon C,Neves RI,Robertson G,Sivik J,Mackley H,Talamo G,Drabick JJ

48-0048 was used in Flow cytometry/Cell sorting to study the effectiveness of different therapy regimes on treating melanoma after failure of immunotherapy.

Tue Mar 01 00:00:00 EST 2016

Exosomes isolated from plasma of glioma patients enrolled in a vaccination trial reflect antitumor immune activity and might predict survival.

Oncoimmunology

Muller L,Muller-Haegele S,Mitsuhashi M,Gooding W,Okada H,Whiteside TL

48-0048 was used in Flow cytometry/Cell sorting to show exosomes from the plasma of glioma patients from a vaccination trial reflect antitumour immune activity which may predict survival.

Mon Jun 01 00:00:00 EDT 2015

Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.

Journal for immunotherapy of cancer

Kovacsovics-Bankowski M,Chisholm L,Vercellini J,Tucker CG,Montler R,Haley D,Newell P,Ma J,Tseng P,Wolf R,Vetto JT,Hammill C,Hansen P,Weinberg AD

48-0048 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of lymphocytes collected from patients with colorectal cancer liver metastases and ovarian cancer.

Mon Dec 01 00:00:00 EST 2014

Signal transducer and activator of transcription 3 (STAT3) mutations underlying autosomal dominant hyper-IgE syndrome impair human CD8(+) T-cell memory formation and function.

The Journal of allergy and clinical immunology

Ives ML,Ma CS,Palendira U,Chan A,Bustamante J,Boisson-Dupuis S,Arkwright PD,Engelhard D,Averbuch D,Magdorf K,Roesler J,Peake J,Wong M,Adelstein S,Choo S,Smart JM,French MA,Fulcher DA,Cook MC,Picard C,Durandy A,Tsumura M,Kobayashi M,Uzel G,Casanova JL,Tangye SG,Deenick EK

48-0048 was used in Flow cytometry/Cell sorting to investigate the role of IL-21 and associated pathways in CD8(+) T-cell immunity in humans, showing that STAT3 mutations impair CD8(+) T-cell memory formation and function.

Thu Aug 01 00:00:00 EDT 2013

Intercellular adhesion molecule 1 mediates migration of Th1 and Th17 cells across human retinal vascular endothelium.

Investigative ophthalmology & visual science

Bharadwaj AS,Schewitz-Bowers LP,Wei L,Lee RW,Smith JR

48-0048 was used in Flow cytometry/Cell sorting to investigate the ability of human Th1- and Th17-polarised cells to cross a simulated human retinal endothelium, and examined the role of IgG superfamily members in this process.

Wed Oct 23 00:00:00 EDT 2013

Inhibition of SLPI ameliorates disease activity in experimental autoimmune encephalomyelitis.

BMC neuroscience

Müller AM,Jun E,Conlon H,Sadiq SA

48-0048 was used in Flow cytometry/Cell sorting to study how SLPI exerts potent pro-inflammatory actions by modulation of T-cell activity and its neutralization may be beneficial for EAE.

Wed Mar 21 00:00:00 EDT 2012

Memory CD8+ T cells from naturally acquired primary dengue virus infection are highly cross-reactive.

Immunology and cell biology

Friberg H,Burns L,Woda M,Kalayanarooj S,Endy TP,Stephens HA,Green S,Rothman AL,Mathew A

48-0048 was used in Flow cytometry/Cell sorting to demonstrate significant DENV serotype-cross-reactivity of CD8+ T-cells after naturally-acquired primary infection.

Sat Jan 01 00:00:00 EST 2011

Memory CD8+ T cells from naturally acquired primary dengue virus infection are highly cross-reactive.

Immunology and cell biology

Friberg H,Burns L,Woda M,Kalayanarooj S,Endy TP,Stephens HA,Green S,Rothman AL,Mathew A

48-0048 was used in Flow cytometry/Cell sorting to demonstrate significant DENV serotype-cross-reactivity of CD8+ T-cells after naturally-acquired primary infection.

Sat Jan 01 00:00:00 EST 2011

Tuberculosis-induced variant IL-4 mRNA encodes a cytokine functioning as growth factor for (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate-specific Vgamma2Vdelta2 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Yuan Z,Wang R,Lee Y,Chen CY,Yu X,Wu Z,Huang D,Shen L,Chen ZW

48-0048 was used in Flow cytometry/Cell sorting to investigate Mycobacterium tuberculosis and bacillus Calmette-Guérin infections in macaques.

Thu Jan 15 00:00:00 EST 2009

Tuberculosis-induced variant IL-4 mRNA encodes a cytokine functioning as growth factor for (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate-specific Vgamma2Vdelta2 T cells.

Journal of immunology (Baltimore, Md. : 1950)

Yuan Z,Wang R,Lee Y,Chen CY,Yu X,Wu Z,Huang D,Shen L,Chen ZW

48-0048 was used in Flow cytometry/Cell sorting to investigate Mycobacterium tuberculosis and bacillus Calmette-Guérin infections in macaques.

Thu Jan 15 00:00:00 EST 2009

Rhesus monkey immature monocyte-derived dendritic cells generate alloantigen-specific regulatory T cells from circulating CD4+CD127-/lo T cells.

Transplantation

Zahorchak AF,Raimondi G,Thomson AW

48-0048 was used in Flow cytometry/Cell sorting to exhibit a novel method for the generation of non-human primate regulatory T cells with alloantigen specificity.

Sun Nov 15 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (OKT4 (OKT-4)), eFluor™ 450, eBioscience™

CD4 Antibody (48-0048-42) in Flow

Product Details

48-0048-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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