Invitrogen

CD4 Monoclonal Antibody (OKT4 (OKT-4)), PerCP-Cyanine5.5, eBioscience™

FIGURE: 1 / 1

CD4 Antibody (45-0048-42) in Flow

Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PerCP-Cyanine5-5 (Product # 45-4732-82) (blue histogram) or Anti-Human CD4 PerCP-Cyanine5-5 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

45-0048-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Human

Published species

Bovine, Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2b, kappa

Recommended Isotype Control

Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT4 (OKT-4)

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10804390

Product Specific Information

Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Gallic acid induces T-helper-1-like T_reg cells and strengthens immune checkpoint blockade efficacy.

Journal for immunotherapy of cancer

Deng B,Yang B,Chen J,Wang S,Zhang W,Guo Y,Han Y,Li H,Dang Y,Yuan Y,Dai X,Zang Y,Li Y,Li B

45-0048-42 was used in Flow cytometry/Cell sorting to provide a new strategy to improve the efficacy of ICB therapy in CRC treatment by inducing Th1-like Foxp3lo Treg cells.

Fri Jul 01 00:00:00 EDT 2022

Elevations of monocyte and neutrophils, and higher levels of granulocyte colony-stimulating factor in peripheral blood in lung cancer patients.

Thoracic cancer

Yin W,Lv J,Yao Y,Zhao Y,He Z,Wang Q,Cui L,Dai H

45-0048-42 was used in Flow Cytometry to assess correlations between immune cell subset profiles in peripheral blood and disease prognosis in patients with lung cancer.

Fri Oct 01 00:00:00 EDT 2021

Boosting with AIDSVAX B/E Enhances Env Constant Region 1 and 2 Antibody-Dependent Cellular Cytotoxicity Breadth and Potency.

Journal of virology

Easterhoff D,Pollara J,Luo K,Tolbert WD,Young B,Mielke D,Jha S,O'Connell RJ,Vasan S,Kim J,Michael NL,Excler JL,Robb ML,Rerks-Ngarm S,Kaewkungwal J,Pitisuttithum P,Nitayaphan S,Sinangil F,Tartaglia J,Phogat S,Kepler TB,Alam SM,Wiehe K,Saunders KO,Montefiori DC,Tomaras GD,Moody MA,Pazgier M,Haynes BF,Ferrari G

45-0048-42 was used in Immunocytochemistry to study HIV vaccine improvement and development.

Fri Jan 31 00:00:00 EST 2020

Repositioning liothyronine for cancer immunotherapy by blocking the interaction of immune checkpoint TIGIT/PVR.

Cell communication and signaling : CCS

Zhou X,Du J,Wang H,Chen C,Jiao L,Cheng X,Zhou X,Chen S,Gou S,Zhao W,Zhai W,Chen J,Gao Y

45-0048 was used in Flow cytometry/Cell sorting to discover how liothyronine could serve as a potential candidate for cancer immunotherapy by blocking the immune checkpoint TIGIT/PVR.

Mon Sep 07 00:00:00 EDT 2020

The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses.

Cancers

Beltrán Hernández I,Angelier ML,Del Buono D'Ondes T,Di Maggio A,Yu Y,Oliveira S

45-0048 was used in Flow cytometry/Cell sorting to explore the potential of NB-PDT to stimulate the immune system.

Wed Apr 15 00:00:00 EDT 2020

Human Fc Receptor-like 3 Inhibits Regulatory T Cell Function and Binds Secretory IgA.

Cell reports

Agarwal S,Kraus Z,Dement-Brown J,Alabi O,Starost K,Tolnay M

45-0048 was used in Flow cytometry/Cell sorting to study the functional regulation of T cells by human Fc receptor-like 3.

Tue Feb 04 00:00:00 EST 2020

TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions.

Cell reports

Leng T,Akther HD,Hackstein CP,Powell K,King T,Friedrich M,Christoforidou Z,McCuaig S,Neyazi M,Arancibia-Cárcamo CV,Hagel J,Powrie F,Peres RS,Millar V,Ebner D,Lamichhane R,Ussher J,Hinks TSC,Marchi E,Willberg C,Klenerman P

45-0048-42 was used in Flow cytometry/Cell sorting to examine the impact of combinations of TCR-dependent and TCR-independent signals in human CD8+ MAIT cells.

Tue Sep 17 00:00:00 EDT 2019

Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5.

BMC biotechnology

Lv Y,Xiao FJ,Wang Y,Zou XH,Wang H,Wang HY,Wang LS,Lu ZZ

45-0048 was used in Flow cytometry/Cell sorting to show the HAdV-11p fiber pseudotyped HAdV-5 could effectively transduce human T cells when human EF1a promoter was used to control the expression of transgene, suggesting its possible application in T cell immunocellular therapy.

Wed Apr 24 00:00:00 EDT 2019

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

45-0048 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 EDT 2019

Dietary, nondigestible oligosaccharides and Bifidobacterium breve M-16V suppress allergic inflammation in intestine via targeting dendritic cell maturation.

Journal of leukocyte biology

de Kivit S,Kostadinova AI,Kerperien J,Morgan ME,Muruzabal VA,Hofman GA,Knippels LMJ,Kraneveld AD,Garssen J,Willemsen LEM

45-0048-42 was used in Flow Cytometry to study whether GF/Bb modulates dendritic cells (DCs) and subsequent Th2 and regulatory T cell (Treg) frequency in the small intestinal lamina propria (SI-LP).

Sat Jul 01 00:00:00 EDT 2017

Creation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells.

PloS one

Zeng Y,Liu B,Rubio MT,Wang X,Ojcius DM,Tang R,Durrbach A,Ru Z,Zhou Y,Lone YC

45-0048 was used in Flow cytometry/Cell sorting to generate a novel immuno-deficient mouse strain expressing human HLA molecules instead of mouse MHC molecules.

Tue Sep 05 00:00:00 EDT 2017

HTLV-1 bZIP Factor Impairs Anti-viral Immunity by Inducing Co-inhibitory Molecule, T Cell Immunoglobulin and ITIM Domain (TIGIT).

PLoS pathogens

Yasuma K,Yasunaga J,Takemoto K,Sugata K,Mitobe Y,Takenouchi N,Nakagawa M,Suzuki Y,Matsuoka M

45-0048 was used in Flow cytometry/Cell sorting to show that HTLV-1 bZIP factor-induced TIGIT plays a pivotal role in attenuating host immune responses and shaping a microenvironment favourable to HTLV-1 in vitro.

Fri Jan 01 00:00:00 EST 2016

Reply to Suppressive activity of human regulatory T cells is maintained in the presence of TNF.

Nature medicine

Nie H,Zheng Y,Li R,Zhang J

45-0048-42 was used in flow cytometry to show that reply to suppressive activity of human regulatory T cells is maintained in the presence of TNF.

Fri Jan 01 00:00:00 EST 2016

Dysbiotic bacteria translocate in progressive SIV infection.

Mucosal immunology

Klase Z,Ortiz A,Deleage C,Mudd JC,Quiñones M,Schwartzman E,Klatt NR,Canary L,Estes JD,Brenchley JM

45-0048 was used in Flow cytometry/Cell sorting to examine the communities of bacteria within the gastrointestinal tract and systemic tissues of both healthy and SIV-infected Asian macaques.

Tue Sep 01 00:00:00 EDT 2015

The Expression of Functional Vpx during Pathogenic SIVmac Infections of Rhesus Macaques Suppresses SAMHD1 in CD4+ Memory T Cells.

PLoS pathogens

Shingai M,Welbourn S,Brenchley JM,Acharya P,Miyagi E,Plishka RJ,Buckler-White A,Kwong PD,Nishimura Y,Strebel K,Martin MA

45-0048 was used in Flow cytometry/Cell sorting to indicate that autologous NAb responses in mothers and infants do not protect against mother to child transmission.

Fri May 01 00:00:00 EDT 2015

Expression of GM-CSF in T Cells Is Increased in Multiple Sclerosis and Suppressed by IFN-β Therapy.

Journal of immunology (Baltimore, Md. : 1950)

Rasouli J,Ciric B,Imitola J,Gonnella P,Hwang D,Mahajan K,Mari ER,Safavi F,Leist TP,Zhang GX,Rostami A

45-0048 was used in Flow cytometry/Cell sorting to characterise GM-CSF production by T cells of MS patients and to determine the effect of IFN-β therapy on its production.

Mon Jun 01 00:00:00 EDT 2015

Large-Scale Purification of r28M: A Bispecific scFv Antibody Targeting Human Melanoma Produced in Transgenic Cattle.

PloS one

Spiesberger K,Paulfranz F,Egger A,Reiser J,Vogl C,Rudolf-Scholik J,Mayrhofer C,Grosse-Hovest L,Brem G

45-0048-42 was used in Flow cytometry/Cell sorting to study how the bispecific scFv antibody r28M produced in transgenic cattle targets human melanoma

Mon Jun 27 00:00:00 EDT 2016

A phase I dose-escalation clinical trial of a peptide-based human papillomavirus therapeutic vaccine with Candida skin test reagent as a novel vaccine adjuvant for treating women with biopsy-proven cervical intraepithelial neoplasia 2/3.

Oncoimmunology

Greenfield WW,Stratton SL,Myrick RS,Vaughn R,Donnalley LM,Coleman HN,Mercado M,Moerman-Herzog AM,Spencer HJ,Andrews-Collins NR,Hitt WC,Low GM,Manning NA,McKelvey SS,Smith D,Smith MV,Phillips AM,Quick CM,Jeffus SK,Hutchins LF,Nakagawa M

45-0048 was used in Flow cytometry/Cell sorting to demonstrate the safety of a human papillomavirus therapeutic vaccine, PepCan.

Thu Oct 01 00:00:00 EDT 2015

Increased peripheral proinflammatory T helper subsets contribute to cardiovascular complications in diabetic patients.

Mediators of inflammation

Zhao RX,Li WJ,Lu YR,Qin J,Wu CL,Tian M,He TY,Yi SN,Tang DQ,Sun L,Chen L

45-0048 was used in Flow cytometry/Cell sorting to assess the frequencies of various immune cells in diabetic patients with or without coronary atherosclerotic heart disease.

Thu Dec 18 00:00:00 EST 2014

Elevated peripheral frequencies of Th22 cells: a novel potent participant in obesity and type 2 diabetes.

PloS one

Zhao R,Tang D,Yi S,Li W,Wu C,Lu Y,Hou X,Song J,Lin P,Chen L,Sun L

45-0048 was used in Flow cytometry/Cell sorting to investigate the potential association between Th22 and the pathogenesis of obesity and type 2 diabetes.

Thu Sep 18 00:00:00 EDT 2014

Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

PLoS pathogens

Lindestam Arlehamn CS,Gerasimova A,Mele F,Henderson R,Swann J,Greenbaum JA,Kim Y,Sidney J,James EA,Taplitz R,McKinney DM,Kwok WW,Grey H,Sallusto F,Peters B,Sette A

45-0048 was used in Flow cytometry/Cell sorting to further define the immunological footprint of Mycobacterium tuberculosis CD4 T cell recognition.

Tue Jan 01 00:00:00 EST 2013

Genome-wide analysis of T cell responses during acute and latent simian varicella virus infections in rhesus macaques.

Journal of virology

Haberthur K,Kraft A,Arnold N,Park B,Meyer C,Asquith M,Dewane J,Messaoudi I

45-0048 was used in Flow cytometry/Cell sorting to characterise the specificity of T cell responses during acute and latent simian varicella virus infection.

Fri Nov 01 00:00:00 EDT 2013

Transcriptional profiling of experimental CD8(+) lymphocyte depletion in rhesus macaques infected with simian immunodeficiency virus SIVmac239.

Journal of virology

Bosinger SE,Jochems SP,Folkner KA,Hayes TL,Klatt NR,Silvestri G

45-0048 was used in Flow cytometry/Cell sorting to identify antiviral pathways perturbed by CD8+ T-cell depletion that may contribute to noncytolytic control of SIV replication.

Tue Jan 01 00:00:00 EST 2013

Assessment of immune interference, antagonism, and diversion following human immunization with biallelic blood-stage malaria viral-vectored vaccines and controlled malaria infection.

Journal of immunology (Baltimore, Md. : 1950)

Elias SC,Collins KA,Halstead FD,Choudhary P,Bliss CM,Ewer KJ,Sheehy SH,Duncan CJ,Biswas S,Hill AV,Draper SJ

45-0048 was used in Flow cytometry/Cell sorting to study the effects of both controlled malaria infection, and human immunisation with biallelic blood-stage malaria viral-vectored vaccines, on the immune system.

Fri Feb 01 00:00:00 EST 2013

Ultradense array CGH and discovery of micro-copy number alterations and gene fusions in the cancer genome.

Methods in molecular biology (Clifton, N.J.)

Przybytkowski E,Aguilar-Mahecha A,Nabavi S,Tonellato PJ,Basik M

45-0048 was used in Immunocytochemistry-immunoflourescence to characterise the protocol steps and analysis required to obtain reliable array comparative array hybridisation results from clinical samples.

Mon Jul 29 00:00:00 EDT 2013

IL-17 and IFN-γ expression in lymphocytes from patients with active tuberculosis correlates with the severity of the disease.

Journal of leukocyte biology

Jurado JO,Pasquinelli V,Alvarez IB,Peña D,Rovetta AI,Tateosian NL,Romeo HE,Musella RM,Palmero D,Chuluyán HE,García VE

45-0048 was used in Flow cytometry/Cell sorting to investigate the induction and regulation of IFN-γ and IL-17 during active human Mycobacterium tuberculosis infection.

Fri Jun 01 00:00:00 EDT 2012

CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.

Journal of immunology (Baltimore, Md. : 1950)

Frahm MA,Picking RA,Kuruc JD,McGee KS,Gay CL,Eron JJ,Hicks CB,Tomaras GD,Ferrari G

45-0048 was used in Flow cytometry/Cell sorting to demonstrate that double-positive T-cells represent a highly reactive cell population during acute HIV infection.

Tue May 01 00:00:00 EDT 2012

Reduced frequency of memory T cells and increased Th17 responses in patients with active tuberculosis.

Clinical and vaccine immunology : CVI

Marín ND,París SC,Rojas M,García LF

45-0048 was used in Flow cytometry/Cell sorting to investigate the alterations in Mycobacterium tuberculosis T cell subsets in patients with active tuberculosis, showing reduced frequency of memory T cells and increased Th17 responses.

Mon Oct 01 00:00:00 EDT 2012

SIV infection of rhesus macaques results in dysfunctional T- and B-cell responses to neo and recall Leishmania major vaccination.

Blood

Klatt NR,Vinton CL,Lynch RM,Canary LA,Ho J,Darrah PA,Estes JD,Seder RA,Moir SL,Brenchley JM

45-0048 was used in Flow cytometry/Cell sorting to show that SIV infection results in dysfunctional T-cell responses to neo and recall vaccinations.

Thu Nov 24 00:00:00 EST 2011

Human CD8⁺ and CD4⁺ T cell memory to lymphocytic choriomeningitis virus infection.

Journal of virology

Kotturi MF,Swann JA,Peters B,Arlehamn CL,Sidney J,Kolla RV,James EA,Akondy RS,Ahmed R,Kwok WW,Buchmeier MJ,Sette A

45-0048 was used in Flow cytometry/Cell sorting to analyse the T cell response to acute lymphocytic choriomeningitis virus infection.

Tue Nov 01 00:00:00 EDT 2011

Mechanisms underlying γδ T-cell subset perturbations in SIV-infected Asian rhesus macaques.

Blood

Harris LD,Klatt NR,Vinton C,Briant JA,Tabb B,Ladell K,Lifson J,Estes JD,Price DA,Hirsch VM,Brenchley JM

45-0048 was used in Flow cytometry/Cell sorting to study differing subsets of γδ T cells in HIV and Simian immunodeficiency virus-infected individuals.

Thu Nov 18 00:00:00 EST 2010

Compromised gastrointestinal integrity in pigtail macaques is associated with increased microbial translocation, immune activation, and IL-17 production in the absence of SIV infection.

Mucosal immunology

Klatt NR,Harris LD,Vinton CL,Sung H,Briant JA,Tabb B,Morcock D,McGinty JW,Lifson JD,Lafont BA,Martin MA,Levine AD,Estes JD,Brenchley JM

45-0048 was used in Flow cytometry/Cell sorting to assess whether high basal levels of immune activation and microbial translocation exist in pigtail macaques.

Thu Jul 01 00:00:00 EDT 2010

Disruption of intestinal CD4+ T cell homeostasis is a key marker of systemic CD4+ T cell activation in HIV-infected individuals.

Journal of immunology (Baltimore, Md. : 1950)

Gordon SN,Cervasi B,Odorizzi P,Silverman R,Aberra F,Ginsberg G,Estes JD,Paiardini M,Frank I,Silvestri G

45-0048 was used in Flow cytometry/Cell sorting to reveal an association between the destruction of intestinal CD4+ T-cell homeostasis and the level of systemic CD4+ T-cell activation.

Mon Nov 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

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CD4 Monoclonal Antibody (OKT4 (OKT-4)), PerCP-Cyanine5.5, eBioscience™

CD4 Antibody (45-0048-42) in Flow

Product Details

45-0048-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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