Invitrogen

CD11c Monoclonal Antibody (N418), eFluor™ 615, eBioscience™

CD11c Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 32

CD11c Antibody (42-0114-82) in IHC (F)

Immunohistochemistry on frozen mouse spleen using 5 µg/mL Anti-Mouse CD11c eFluor® 615. Nuclei are counterstained with DAPI.

CD11c Antibody in Immunohistochemistry (IHC)

CD11c Antibody in Immunocytochemistry (ICC/IF)

CD11c Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

Product Image Gallery

Product Details

42-0114-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 20 publications 20 publications

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 5 publications 5 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), eFluor™ 615, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

N418

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10853016

Product Specific Information

Description: The N418 monoclonal antibody reacts with mouse CD11c, the integrin alpha X. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported: This N418 antibody has been reported for use in immunohistochemical staining of frozen tissue sections and immunocytochemistry.

Applications Tested: This N418 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 5 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength. (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeted inhibition of osteoclastogenesis reveals the pathogenesis and therapeutics of bone loss under sympathetic neurostress.

International journal of oral science

Sui B,Liu J,Zheng C,Dang L,Chen J,Cao Y,Zhang K,Liu L,Dang M,Zhang L,Chen N,He T,Xuan K,Jin F,Zhang G,Jin Y,Hu C

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Mon Aug 01 00:00:00 EDT 2022

Self-Tolerance of Vascular Tissues Is Broken Down by Vascular Dendritic Cells in Response to Systemic Inflammation to Initiate Regional Autoinflammation.

Frontiers in immunology

Sun L,Zhang W,Zhao L,Zhao Y,Wang F,Lew AM,Xu Y

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2022

Single-cell transcriptomics reveals distinct cell response between acute and chronic pulmonary infection of Pseudomonas aeruginosa.

MedComm

Hu X,Wu M,Ma T,Zhang Y,Zou C,Wang R,Zhang Y,Ren Y,Li Q,Liu H,Li H,Wang T,Sun X,Yang Y,Tang M,Li X,Li J,Gao X,Li T,Zhou X

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Thu Dec 01 00:00:00 EST 2022

Neutrophil-specific gain-of-function mutations in Nlrp3 promote development of cryopyrin-associated periodic syndrome.

The Journal of experimental medicine

Stackowicz J,Gaudenzio N,Serhan N,Conde E,Godon O,Marichal T,Starkl P,Balbino B,Roers A,Bruhns P,Jönsson F,Moguelet P,Georgin-Lavialle S,Broderick L,Hoffman HM,Galli SJ,Reber LL

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Mon Oct 04 00:00:00 EDT 2021

Depletion of Adipocyte Becn1 Leads to Lipodystrophy and Metabolic Dysregulation.

Diabetes

Jin Y,Ji Y,Song Y,Choe SS,Jeon YG,Na H,Nam TW,Kim HJ,Nahmgoong H,Kim SM,Kim JW,Nam KT,Seong JK,Hwang D,Park CB,Lee IH,Kim JB,Lee HW

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Fri Jan 01 00:00:00 EST 2021

Enrichment of Neurodegenerative Microglia Signature in Brain-Derived Extracellular Vesicles Isolated from Alzheimer's Disease Mouse Models.

Journal of proteome research

Muraoka S,Jedrychowski MP,Iwahara N,Abdullah M,Onos KD,Keezer KJ,Hu J,Ikezu S,Howell GR,Gygi SP,Ikezu T

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Western Blot

Fri Mar 05 00:00:00 EST 2021

Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis.

Nano-micro letters

Zhao Q,Shi M,Yin C,Zhao Z,Zhang J,Wang J,Shen K,Zhang L,Tang H,Xiao Y,Zhang Y

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Sat Nov 21 00:00:00 EST 2020

Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages.

EMBO molecular medicine

Menga A,Serra M,Todisco S,Riera-Domingo C,Ammarah U,Ehling M,Palmieri EM,Di Noia MA,Gissi R,Favia M,Pierri CL,Porporato PE,Castegna A,Mazzone M

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Wed Oct 07 00:00:00 EDT 2020

Endothelial-to-mesenchymal transition compromises vascular integrity to induce Myc-mediated metabolic reprogramming in kidney fibrosis.

Science signaling

Lovisa S,Fletcher-Sananikone E,Sugimoto H,Hensel J,Lahiri S,Hertig A,Taduri G,Lawson E,Dewar R,Revuelta I,Kato N,Wu CJ,Bassett RL,Putluri N,Zeisberg M,Zeisberg EM,LeBleu VS,Kalluri R

42-0114 was used in Flow cytometry/Cell sorting to demonstrate a functional role for EndMT in the response to kidney capillary endothelial injury and highlight the contribution of endothelial-epithelial cross-talk in the development of kidney fibrosis with a potential for therapeutic intervention.

Tue Jun 09 00:00:00 EDT 2020

Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.

Science translational medicine

Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Wed Apr 12 00:00:00 EDT 2017

Identification of different macrophage subpopulations with distinct activities in a mouse model of oxygen-induced retinopathy.

International journal of molecular medicine

Zhu Y,Zhang L,Lu Q,Gao Y,Cai Y,Sui A,Su T,Shen X,Xie B

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Tue Aug 01 00:00:00 EDT 2017

PEDF mediates pathological neovascularization by regulating macrophage recruitment and polarization in the mouse model of oxygen-induced retinopathy.

Scientific reports

Gao S,Li C,Zhu Y,Wang Y,Sui A,Zhong Y,Xie B,Shen X

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Fri Feb 17 00:00:00 EST 2017

CD11c+ resident macrophages drive hepatocyte death-triggered liver fibrosis in a murine model of nonalcoholic steatohepatitis.

JCI insight

Itoh M,Suganami T,Kato H,Kanai S,Shirakawa I,Sakai T,Goto T,Asakawa M,Hidaka I,Sakugawa H,Ohnishi K,Komohara Y,Asano K,Sakaida I,Tanaka M,Ogawa Y

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Thu Nov 16 00:00:00 EST 2017

Precision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells.

Journal of visualized experiments : JoVE

Lyons-Cohen MR,Thomas SY,Cook DN,Nakano H

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Wed Apr 05 00:00:00 EDT 2017

Identification of a Hematopoietic Cell Dedifferentiation-Inducing Factor.

Journal of cellular physiology

Li Y,Adomat H,Guns ET,Hojabrpour P,Duronio V,Curran TA,Jalili RB,Jia W,Delwar Z,Zhang Y,Elizei SS,Ghahary A

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunocytochemistry

Wed Jun 01 00:00:00 EDT 2016

Flagellin is a strong vaginal adjuvant of a therapeutic vaccine for genital cancer.

Oncoimmunology

Lee SE,Hong SH,Verma V,Lee YS,Duong TN,Jeong K,Uthaman S,Sung YC,Lee JT,Park IK,Min JJ,Rhee JH

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Mon Feb 01 00:00:00 EST 2016

Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis.

Biochimica et biophysica acta

Hersrud SL,Kovács AD,Pearce DA

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunocytochemistry

Fri Jul 01 00:00:00 EDT 2016

M cell-derived vesicles suggest a unique pathway for trans-epithelial antigen delivery.

Tissue barriers

Sakhon OS,Ross B,Gusti V,Pham AJ,Vu K,Lo DD

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Fri Apr 03 00:00:00 EDT 2015

Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes.

Nature medicine

Clatworthy MR,Aronin CE,Mathews RJ,Morgan NY,Smith KG,Germain RN

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Mon Dec 01 00:00:00 EST 2014

Sessile alveolar macrophages communicate with alveolar epithelium to modulate immunity.

Nature

Westphalen K,Gusarova GA,Islam MN,Subramanian M,Cohen TS,Prince AS,Bhattacharya J

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Thu Feb 27 00:00:00 EST 2014

Mice deficient in intestinal epithelium cytochrome P450 reductase are prone to acute toxin-induced mucosal damage.

Scientific reports

Ahlawat S,Xie F,Zhu Y,D'Hondt R,Ding X,Zhang QY,Mantis NJ

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Thu Jul 03 00:00:00 EDT 2014

Invariant natural killer T cells regulate anti-tumor immunity by controlling the population of dendritic cells in tumor and draining lymph nodes.

Journal for immunotherapy of cancer

Pilones KA,Aryankalayil J,Babb JS,Demaria S

42-0114 was used in Immunohistochemistry to demonstrate that iNKT cells limit priming of anti-tumor T-cells by controlling dendritic cells in tumors and draining lymph nodes.

Tue Oct 28 00:00:00 EDT 2014

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Miscellaneous

Tue Jan 01 00:00:00 EST 2013

Hepatic crown-like structure: a unique histological feature in non-alcoholic steatohepatitis in mice and humans.

PloS one

Itoh M,Kato H,Suganami T,Konuma K,Marumoto Y,Terai S,Sakugawa H,Kanai S,Hamaguchi M,Fukaishi T,Aoe S,Akiyoshi K,Komohara Y,Takeya M,Sakaida I,Ogawa Y

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Sun Oct 12 00:00:00 EDT 2014

Depletion of B2 but not B1a B cells in BAFF receptor-deficient ApoE mice attenuates atherosclerosis by potently ameliorating arterial inflammation.

PloS one

Kyaw T,Tay C,Hosseini H,Kanellakis P,Gadowski T,MacKay F,Tipping P,Bobik A,Toh BH

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunohistochemistry

Mon May 14 00:00:00 EDT 2012

Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.

Journal of neurochemistry

Koronyo-Hamaoui M,Ko MK,Koronyo Y,Azoulay D,Seksenyan A,Kunis G,Pham M,Bakhsheshian J,Rogeri P,Black KL,Farkas DL,Schwartz M

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Tue Dec 01 00:00:00 EST 2009

Leptin receptor is expressed in thymus medulla and leptin protects against thymic remodeling during endotoxemia-induced thymus involution.

The Journal of endocrinology

Gruver AL,Ventevogel MS,Sempowski GD

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2009

Dynamics of lung macrophage activation in response to helminth infection.

Journal of leukocyte biology

Siracusa MC,Reece JJ,Urban JF,Scott AL

Published figure using CD11c monoclonal antibody (Product # 42-0114-82) in Immunofluorescence

Mon Dec 01 00:00:00 EST 2008

Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo.

The Journal of experimental medicine

Ohteki T,Tada H,Ishida K,Sato T,Maki C,Yamada T,Hamuro J,Koyasu S

Mon Oct 02 00:00:00 EDT 2006

Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo.

The Journal of experimental medicine

Ohteki T,Tada H,Ishida K,Sato T,Maki C,Yamada T,Hamuro J,Koyasu S

Mon Oct 02 00:00:00 EDT 2006

The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties.

International immunology

Zhang J,Kawashima N,Suda H,Nakano Y,Takano Y,Azuma M

Fri Sep 01 00:00:00 EDT 2006

Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection.

Cancer research

Guiducci C,Vicari AP,Sangaletti S,Trinchieri G,Colombo MP

Fri Apr 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD11 antigen-like family member C; CD11c; CD11C (p150) alpha polypeptide; complement receptor 4; Integrin alpha-X; integrin aX; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95

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Gene Aliases: AI449405; Cd11c; Cr4; Itgax; N418

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UniProt ID: (Mouse) Q9QXH4

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Entrez Gene ID: (Mouse) 16411

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Function(s)

metal ion binding integrin

Process(es)

cell adhesion integrin-mediated signaling pathway heterotypic cell-cell adhesion activated T cell proliferation defense response to virus positive regulation of protein targeting to mitochondrion

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD11c Monoclonal Antibody (N418), eFluor™ 615, eBioscience™

CD11c Antibody (42-0114-82) in IHC (F)

Product Details

42-0114-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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