Invitrogen

MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience™

MHC Class II (I-A/I-E) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 98

MHC Class II (I-A/I-E) Antibody (16-5321-81) in Flow

Staining of mouse splenocytes with Anti-Mouse MHC Class II (I-A/I-E) FITC (left) and APC (right).Appropriate isotype controls were used (open histogram).Total viable cells were used for analysis.

MHC Class II (I-A/I-E) Antibody in Immunocytochemistry (ICC/IF)

MHC Class II (I-A/I-E) Antibody in Immunocytochemistry, Immunohistochemistry, Flow Cytometry (ICC/IF, IHC, Flow)

MHC Class II (I-A/I-E) Antibody in Immunohistochemistry (IHC)

MHC Class II (I-A/I-E) Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Product Details

16-5321-81

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 21 publications 21 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.125 µg/test

View 85 publications 85 publications

Immunoprecipitation (IP)

View 2 publications 2 publications

Neutralization (Neu)

Assay-Dependent

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Immunomicroscopy (IM)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M5/114.15.2

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469098

Product Specific Information

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.

Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis and in vitro blocking of T cell proliferative responses.

Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

Frontiers in immunology

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E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions.

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Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

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Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

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Theranostics

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Scientific reports

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Polyanhydride Nanoparticles Induce Low Inflammatory Dendritic Cell Activation Resulting in CD8⁺ T Cell Memory and Delayed Tumor Progression.

International journal of nanomedicine

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cGAS/STING axis mediates a topoisomerase II inhibitor-induced tumor immunogenicity.

The Journal of clinical investigation

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Functional antagonism of sphingosine-1-phosphate receptor 1 prevents cuprizone-induced demyelination.

Glia

Kim S,Bielawski J,Yang H,Kong Y,Zhou B,Li J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Thu Mar 01 00:00:00 EST 2018

Functional antagonism of sphingosine-1-phosphate receptor 1 prevents cuprizone-induced demyelination.

Glia

Kim S,Bielawski J,Yang H,Kong Y,Zhou B,Li J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Thu Mar 01 00:00:00 EST 2018

Age Influences Microglial Activation After Cuprizone-Induced Demyelination.

Frontiers in aging neuroscience

Klein B,Mrowetz H,Barker CM,Lange S,Rivera FJ,Aigner L

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2020

Effect of immune tolerance induced by immature dendritic cells and CTLA4-Ig on systemic lupus erythematosus: An in vivo study.

Experimental and therapeutic medicine

Huang C,Zhang L,Ling F,Wen S,Luo Y,Liu H,Liu J,Zheng W,Liang M,Sun J,Lin YK

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F.

Journal of immunological methods

Geslewitz WE,Percopo CM,Rosenberg HF

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Myeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation.

Nature communications

Bonacina F,Coe D,Wang G,Longhi MP,Baragetti A,Moregola A,Garlaschelli K,Uboldi P,Pellegatta F,Grigore L,Da Dalt L,Annoni A,Gregori S,Xiao Q,Caruso D,Mitro N,Catapano AL,Marelli-Berg FM,Norata GD

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Mon Aug 06 00:00:00 EDT 2018

CD4⁺ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema.

Nature communications

García Nores GD,Ly CL,Cuzzone DA,Kataru RP,Hespe GE,Torrisi JS,Huang JJ,Gardenier JC,Savetsky IL,Nitti MD,Yu JZ,Rehal S,Mehrara BJ

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu May 17 00:00:00 EDT 2018

Lin^-CCR2⁺ hematopoietic stem and progenitor cells overcome resistance to PD-1 blockade.

Nature communications

Flores CT,Wildes TJ,Drake JA,Moore GL,Dean BD,Abraham RS,Mitchell DA

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Wed Oct 17 00:00:00 EDT 2018

Lin^-CCR2⁺ hematopoietic stem and progenitor cells overcome resistance to PD-1 blockade.

Nature communications

Flores CT,Wildes TJ,Drake JA,Moore GL,Dean BD,Abraham RS,Mitchell DA

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Wed Oct 17 00:00:00 EDT 2018

A CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.

The Journal of clinical investigation

Berlato C,Khan MN,Schioppa T,Thompson R,Maniati E,Montfort A,Jangani M,Canosa M,Kulbe H,Hagemann UB,Duncan AR,Fletcher L,Wilkinson RW,Powles T,Quezada SA,Balkwill FR

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Prolonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells.

Frontiers in immunology

Bai WJ,Li CG,Zhang CC,Xu LH,Zeng QZ,Hu B,Hong Z,He XH,Ouyang DY

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Loss of IRF2BP2 in Microglia Increases Inflammation and Functional Deficits after Focal Ischemic Brain Injury.

Frontiers in cellular neuroscience

Cruz SA,Hari A,Qin Z,Couture P,Huang H,Lagace DC,Stewart AFR,Chen HH

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis.

PloS one

McDonald LT,Johnson SD,Russell DL,Young MRI,LaRue AC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Mon Sep 25 00:00:00 EDT 2017

Heavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease.

Scientific reports

Ogawa Y,He H,Mukai S,Imada T,Nakamura S,Su CW,Mahabole M,Tseng SC,Tsubota K

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Mon Feb 06 00:00:00 EST 2017

Microglial brain region-dependent diversity and selective regional sensitivities to aging.

Nature neuroscience

Grabert K,Michoel T,Karavolos MH,Clohisey S,Baillie JK,Stevens MP,Freeman TC,Summers KM,McColl BW

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Interleukin-13 immune gene therapy prevents CNS inflammation and demyelination via alternative activation of microglia and macrophages.

Glia

Guglielmetti C,Le Blon D,Santermans E,Salas-Perdomo A,Daans J,De Vocht N,Shah D,Hoornaert C,Praet J,Peerlings J,Kara F,Bigot C,Mai Z,Goossens H,Hens N,Hendrix S,Verhoye M,Planas AM,Berneman Z,van der Linden A,Ponsaerts P

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Thu Dec 01 00:00:00 EST 2016

Microglia are less pro-inflammatory than myeloid infiltrates in the hippocampus of mice exposed to status epilepticus.

Glia

Vinet J,Vainchtein ID,Spano C,Giordano C,Bordini D,Curia G,Dominici M,Boddeke HW,Eggen BJ,Biagini G

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Mon Aug 01 00:00:00 EDT 2016

Opposite Effects of Coinjection and Distant Injection of Mesenchymal Stem Cells on Breast Tumor Cell Growth.

Stem cells translational medicine

Zheng H,Zou W,Shen J,Xu L,Wang S,Fu YX,Fan W

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

Macrophages transfer antigens to dendritic cells by releasing exosomes containing dead-cell-associated antigens partially through a ceramide-dependent pathway to enhance CD4(+) T-cell responses.

Immunology

Xu Y,Liu Y,Yang C,Kang L,Wang M,Hu J,He H,Song W,Tang H

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Recruited monocytes modulate malaria-induced lung injury through CD36-mediated clearance of sequestered infected erythrocytes.

Journal of leukocyte biology

Lagassé HA,Anidi IU,Craig JM,Limjunyawong N,Poupore AK,Mitzner W,Scott AL

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Lymphatic endothelial lineage assemblage during corneal lymphangiogenesis.

Laboratory investigation; a journal of technical methods and pathology

Connor AL,Kelley PM,Tempero RM

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Tue Mar 01 00:00:00 EST 2016

Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.

Journal of immunology (Baltimore, Md. : 1950)

Menendez CM,Jinkins JK,Carr DJ

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Mon Aug 15 00:00:00 EDT 2016

Effects of ketanserin on experimental colitis in mice and macrophage function.

International journal of molecular medicine

Xiao J,Shao L,Shen J,Jiang W,Feng Y,Zheng P,Liu F

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

Repulsive guidance molecule a blockade exerts the immunoregulatory function in DCs stimulated with ABP and LPS.

Human vaccines & immunotherapeutics

Xu X,Gao Y,Zhai Z,Zhang S,Shan F,Feng J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Tue Aug 02 00:00:00 EDT 2016

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

American journal of translational research

Wang H,Hu W,Liang Z,Zeng L,Li J,Yan H,Yang P,Liu Z,Wang L

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Allergy Enhances Neurogenesis and Modulates Microglial Activation in the Hippocampus.

Frontiers in cellular neuroscience

Klein B,Mrowetz H,Thalhamer J,Scheiblhofer S,Weiss R,Aigner L

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Fri Jul 22 00:00:00 EDT 2016

Stroke sensitivity in the aged: sex chromosome complement vs. gonadal hormones.

Aging

McCullough LD,Mirza MA,Xu Y,Bentivegna K,Steffens EB,Ritzel R,Liu F

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Epidermal RAF prevents allergic skin disease.

eLife

Raguz J,Jeric I,Niault T,Nowacka JD,Kuzet SE,Rupp C,Fischer I,Biggi S,Borsello T,Baccarini M

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model.

Journal of neuroinflammation

Le Blon D,Guglielmetti C,Hoornaert C,Quarta A,Daans J,Dooley D,Lemmens E,Praet J,De Vocht N,Reekmans K,Santermans E,Hens N,Goossens H,Verhoye M,Van der Linden A,Berneman Z,Hendrix S,Ponsaerts P

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Wed Nov 09 00:00:00 EST 2016

Internalization and presentation of myelin antigens by the brain endothelium guides antigen-specific T cell migration.

eLife

Lopes Pinheiro MA,Kamermans A,Garcia-Vallejo JJ,van Het Hof B,Wierts L,O'Toole T,Boeve D,Verstege M,van der Pol SM,van Kooyk Y,de Vries HE,Unger WW

16-5321 was used in Blocking experiments to demonstrate that endothelial cells derived from the brain are capable of enhancing antigen-specific T cell recruitment.

Thu Jun 23 00:00:00 EDT 2016

Internalization and presentation of myelin antigens by the brain endothelium guides antigen-specific T cell migration.

eLife

Lopes Pinheiro MA,Kamermans A,Garcia-Vallejo JJ,van Het Hof B,Wierts L,O'Toole T,Boeve D,Verstege M,van der Pol SM,van Kooyk Y,de Vries HE,Unger WW

16-5321 was used in Blocking experiments to demonstrate that endothelial cells derived from the brain are capable of enhancing antigen-specific T cell recruitment.

Thu Jun 23 00:00:00 EDT 2016

Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor.

Scientific reports

Brandstätter O,Schanz O,Vorac J,König J,Mori T,Maruyama T,Korkowski M,Haarmann-Stemmann T,von Smolinski D,Schultze JL,Abel J,Esser C,Takeyama H,Weighardt H,Förster I

16-5321 was used in Flow cytometry/Cell sorting to highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli.

Tue May 17 00:00:00 EDT 2016

Adaptive Immune Regulation of Mammary Postnatal Organogenesis.

Developmental cell

Plaks V,Boldajipour B,Linnemann JR,Nguyen NH,Kersten K,Wolf Y,Casbon AJ,Kong N,van den Bijgaart RJ,Sheppard D,Melton AC,Krummel MF,Werb Z

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Mon Sep 14 00:00:00 EDT 2015

Benefits of Neuronal Preferential Systemic Gene Therapy for Neurotransmitter Deficiency.

Molecular therapy : the journal of the American Society of Gene Therapy

Lee NC,Muramatsu S,Chien YH,Liu WS,Wang WH,Cheng CH,Hu MK,Chen PW,Tzen KY,Byrne BJ,Hwu WL

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunohistochemistry

Thu Oct 01 00:00:00 EDT 2015

The late endosomal adaptor molecule p14 (LAMTOR2) regulates TGFβ1-mediated homeostasis of Langerhans cells.

The Journal of investigative dermatology

Sparber F,Tripp CH,Komenda K,Scheffler JM,Clausen BE,Huber LA,Romani N,Stoitzner P

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Reducing progression of experimental lupus nephritis via inhibition of the B7/CD28 signaling pathway.

Molecular medicine reports

Huang L,Kong Y,Wang J,Sun J,Shi Q,Qiu YH

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2015

Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.

Cell reports

DeFalco T,Potter SJ,Williams AV,Waller B,Kan MJ,Capel B

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Tue Aug 18 00:00:00 EDT 2015

Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.

Cell reports

DeFalco T,Potter SJ,Williams AV,Waller B,Kan MJ,Capel B

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Tue Aug 18 00:00:00 EDT 2015

Macrophages Contribute to the Spermatogonial Niche in the Adult Testis.

Cell reports

DeFalco T,Potter SJ,Williams AV,Waller B,Kan MJ,Capel B

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Tue Aug 18 00:00:00 EDT 2015

Sessile alveolar macrophages communicate with alveolar epithelium to modulate immunity.

Nature

Westphalen K,Gusarova GA,Islam MN,Subramanian M,Cohen TS,Prince AS,Bhattacharya J

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Thu Feb 27 00:00:00 EST 2014

High-fat-diet-mediated dysbiosis promotes intestinal carcinogenesis independently of obesity.

Nature

Schulz MD,Atay C,Heringer J,Romrig FK,Schwitalla S,Aydin B,Ziegler PK,Varga J,Reindl W,Pommerenke C,Salinas-Riester G,Böck A,Alpert C,Blaut M,Polson SC,Brandl L,Kirchner T,Greten FR,Polson SW,Arkan MC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Oct 23 00:00:00 EDT 2014

C/EBPβ expression is an independent predictor of overall survival in breast cancer patients by MHCII/CD4-dependent mechanism of metastasis formation.

Oncogenesis

Kurzejamska E,Johansson J,Jirström K,Prakash V,Ananthaseshan S,Boon L,Fuxe J,Religa P

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunohistochemistry

Mon Nov 03 00:00:00 EST 2014

Internalization and TLR-dependent type I interferon production by monocytes in response to Toxoplasma gondii.

Immunology and cell biology

Han SJ,Melichar HJ,Coombes JL,Chan SW,Koshy AA,Boothroyd JC,Barton GM,Robey EA

16-5321 was used in Flow cytometry/Cell sorting to show that inflammatory monocytes, but not neutrophils, produce IFN-β in response to T. gondii infection.

Sat Nov 01 00:00:00 EDT 2014

Innate immune cell CD45 regulates lymphopenia-induced T cell proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Saunders AE,Shim YA,Johnson P

16-5321 was used in Flow cytometry/Cell sorting to demonstrate a novel role for CD45 on innate immune cells in promoting lymphopenia-induced T cell proliferation.

Mon Sep 15 00:00:00 EDT 2014

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

An ENU-induced splicing mutation reveals a role for Unc93b1 in early immune cell activation following influenza A H1N1 infection.

Genes and immunity

Lafferty EI,Flaczyk A,Angers I,Homer R,d'Hennezel E,Malo D,Piccirillo CA,Vidal SM,Qureshi ST

16-5321 was used in Flow cytometry/Cell sorting to establish a role for Unc93b1 and endosomal TLRs in the activation of both myeloid and lymphoid cells during the innate immune response to influenza.

Fri Apr 10 00:00:00 EDT 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunoprecipitation

Wed Jan 01 00:00:00 EST 2014

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunoprecipitation

Wed Jan 01 00:00:00 EST 2014

Assessing the role of STAT3 in DC differentiation and autologous DC immunotherapy in mouse models of GBM.

PloS one

Assi H,Espinosa J,Suprise S,Sofroniew M,Doherty R,Zamler D,Lowenstein PR,Castro MG

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Fri Jan 23 00:00:00 EST 2015

Assessing the role of STAT3 in DC differentiation and autologous DC immunotherapy in mouse models of GBM.

PloS one

Assi H,Espinosa J,Suprise S,Sofroniew M,Doherty R,Zamler D,Lowenstein PR,Castro MG

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Fri Jan 23 00:00:00 EST 2015

Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy.

PloS one

Xiao GY,Liu IH,Cheng CC,Chang CC,Lee YH,Cheng WT,Wu SC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu May 28 00:00:00 EDT 2015

Tetanus toxoid-pulsed monocyte vaccination for augmentation of collateral vessel growth.

Journal of the American Heart Association

Herold J,Francke A,Weinert S,Schmeisser A,Hebel K,Schraven B,Roehl FW,Strasser RH,Braun-Dullaeus RC

16-5321 was used in Flow cytometry/Cell sorting to investigate the effect of tetanus toxoid on augmentation of arteriogenesis.

Mon Apr 14 00:00:00 EDT 2014

The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain.

The Journal of experimental medicine

Schneppenheim J,Dressel R,Hüttl S,Lüllmann-Rauch R,Engelke M,Dittmann K,Wienands J,Eskelinen EL,Hermans-Borgmeyer I,Fluhrer R,Saftig P,Schröder B

16-5321 was used in Immuno-electron microscopy to investigate the role of SPPL2a in the cleavage of type 2 transmembrane proteins, showing that it promotes B cell development and controls endosomal traffic.

Mon Jan 14 00:00:00 EST 2013

The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain.

The Journal of experimental medicine

Schneppenheim J,Dressel R,Hüttl S,Lüllmann-Rauch R,Engelke M,Dittmann K,Wienands J,Eskelinen EL,Hermans-Borgmeyer I,Fluhrer R,Saftig P,Schröder B

Mon Jan 14 00:00:00 EST 2013

The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain.

The Journal of experimental medicine

Schneppenheim J,Dressel R,Hüttl S,Lüllmann-Rauch R,Engelke M,Dittmann K,Wienands J,Eskelinen EL,Hermans-Borgmeyer I,Fluhrer R,Saftig P,Schröder B

Mon Jan 14 00:00:00 EST 2013

Endothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages.

Blood

He H,Xu J,Warren CM,Duan D,Li X,Wu L,Iruela-Arispe ML

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Thu Oct 11 00:00:00 EDT 2012

Preferential colonization of metastases by oncolytic vaccinia virus strain GLV-1h68 in a human PC-3 prostate cancer model in nude mice.

PloS one

Donat U,Weibel S,Hess M,Stritzker J,Härtl B,Sturm JB,Chen NG,Gentschev I,Szalay AA

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunofluorescence

Tue May 07 00:00:00 EDT 2013

Isolation and characterization of novel murine epiphysis derived mesenchymal stem cells.

PloS one

Cheng CC,Lian WS,Hsiao FS,Liu IH,Lin SP,Lee YH,Chang CC,Xiao GY,Huang HY,Cheng CF,Cheng WT,Wu SC

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2012

Comparison of gene expression profiles between human and mouse monocyte subsets.

Blood

Ingersoll MA,Spanbroek R,Lottaz C,Gautier EL,Frankenberger M,Hoffmann R,Lang R,Haniffa M,Collin M,Tacke F,Habenicht AJ,Ziegler-Heitbrock L,Randolph GJ

16-5321 was used in Flow cytometry/Cell sorting to identify how human and mouse monocyte subsets are far more broadly conserved than currently recognised.

Thu Jan 21 00:00:00 EST 2010

Naive tumor-specific CD4(+) T cells differentiated in vivo eradicate established melanoma.

The Journal of experimental medicine

Xie Y,Akpinarli A,Maris C,Hipkiss EL,Lane M,Kwon EK,Muranski P,Restifo NP,Antony PA

16-5321 was used in Flow cytometry/Cell sorting to study the regression of tumours due to naive tumor-specific CD4(+) T cell differentiation.

Mon Mar 15 00:00:00 EDT 2010

Combination of all-trans retinoic acid and a human papillomavirus therapeutic vaccine suppresses the number and function of immature myeloid cells and enhances antitumor immunity.

Cancer science

Song X,Ye D,Liu B,Cui J,Zhao X,Yi L,Liang J,Song J,Zhang Z,Zhao Q

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2009

Cellular expression and antimicrobial function of a phylogenetically conserved novel histone 1x-like protein on mouse cells: a potential new class of pattern recognition receptor.

Journal of leukocyte biology

Evans DL,Connor MA,Moss LD,Lackay S,Leary JH,Krunkosky T,Jaso-Friedmann L

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2009

Allogeneic stromal cell implantation in brain tissue leads to robust microglial activation.

Immunology and cell biology

Tambuyzer BR,Bergwerf I,De Vocht N,Reekmans K,Daans J,Jorens PG,Goossens H,Ysebaert DK,Chatterjee S,Van Marck E,Berneman ZN,Ponsaerts P

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2009

Mycobacterium bovis BCG decreases MHC-II expression in vivo on murine lung macrophages and dendritic cells during aerosol infection.

Cellular immunology

Pecora ND,Fulton SA,Reba SM,Drage MG,Simmons DP,Urankar-Nagy NJ,Boom WH,Harding CV

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Flow Cytometry

Fri Jan 02 00:00:00 EST 2009

Infiltration of inflammatory cells plays an important role in matrix metalloproteinase expression and activation in the heart during sepsis.

The American journal of pathology

Cuenca J,Martín-Sanz P,Alvarez-Barrientos AM,Boscá L,Goren N

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunohistochemistry

Wed Nov 01 00:00:00 EST 2006

Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract.

Infection and immunity

Gueirard P,Ave P,Balazuc AM,Thiberge S,Huerre M,Milon G,Guiso N

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunohistochemistry

Tue Jul 01 00:00:00 EDT 2003

Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis).

The American journal of pathology

Bagavant H,Sharp C,Kurth B,Tung KS

Published figure using MHC Class II (I-A/I-E) monoclonal antibody (Product # 16-5321-81) in Immunohistochemistry

Tue Jan 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: A alpha; cell surface glycoprotein; H-2 class II histocompatibility antigen, A beta chain; H-2 class II histocompatibility antigen, A-B alpha chain; H-2 class II histocompatibility antigen, A-F alpha chain; H-2 class II histocompatibility antigen, A-K alpha chain; H-2 class II histocompatibility antigen, A-Q alpha chain; H-2 class II histocompatibility antigen, A-R alpha chain; H-2 class II histocompatibility antigen, A-S alpha chain; H-2 class II histocompatibility antigen, A-U alpha chain; H-2 class II histocompatibility antigen, I-E beta chain; histocompatibility 2, class II antigen A, alpha; histocompatibility 2, class II antigen A, beta 1; I-Ab; I-Ad; I-Aq; I-E beta MHC class II; I-Ed; I-Ek; IAalpha; major histocompatibility complex class II beta chain; major histocompatibility complex H-2E beta chain; major histocompatibility protein class II alpha chain; MHC class 2; MHC class II antigen A alpha isoform-004; MHC class II antigen A beta; MHC class II H2-IA-beta-psi; MHC class II IE-b; MHC H2-IE-beta cell surface glycoprotein; MHC-II I-A(d) alpha; response to metastatic cancers 1

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Gene Aliases: Aalpha; Abeta; AI845868; H-2Aa; H-2Ab; H-2Eb; H2-Aa; H2-Ab; H2-Ab1; H2-iabeta; H2Aa; H2Eb; I-Aalpha; I-Abeta; Ia-1; Ia-2; Ia-4; Ia1; Ia2; Ia4; IAalpha; IAb; Rmcs1

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UniProt ID: (Mouse) P14434, (Mouse) P14483

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Entrez Gene ID: (Mouse) 14960, (Mouse) 14961, (Mouse) 14969

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Function(s)

protein binding peptide antigen binding protein heterodimerization activity transcription factor binding toxic substance binding ubiquitin protein ligase binding repressing transcription factor binding major histocompatibility complex protein

Process(es)

immune system process antigen processing and presentation of peptide or polysaccharide antigen via MHC class II immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class II response to interferon-gamma negative regulation of T cell proliferation positive regulation of T cell differentiation antigen processing and presentation of peptide antigen B cell affinity maturation immunoglobulin production involved in immunoglobulin mediated immune response humoral immune response mediated by circulating immunoglobulin positive regulation of antigen processing and presentation positive regulation of T-helper 1 type immune response positive regulation of alpha-beta T cell activation cellular response to interferon-gamma

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MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Functional Grade, eBioscience™

MHC Class II (I-A/I-E) Antibody (16-5321-81) in Flow

Product Details

16-5321-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Immunomicroscopy (IM)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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