Invitrogen Quant-iT assay kits use intentionally designed fluorescent dyes which bind selectively to DNA, RNA, protein, or endotoxin. The intensity of the fluorescent signal of the resulting complex is proportional to the amount of the target molecule in the sample.

Key benefits of Quant-iT assays:

  • Accuracy—Quant-iT technology gives more accurate results than can be obtained with UV absorbance readings, because the Quant-iT reagents only report the molecule of interest—there’s no interference from free nucleotides or other contaminants.
  • Sensitivity—Upon binding to DNA, RNA, or protein, the fluorescence of the Quant-iT dyes increases several hundred fold, giving a very high signal-to-background ratio resulting in accurate low-concentration measurements—up to 1,000 times more sensitive than absorbance-based readings.
  • Confidence—Confidence comes from knowing your assay is sensitive and accurate enough to quantitate even the most dilute or low-abundance samples, while still leaving enough of your biomolecule for successful downstream applications. With Quant-iT technology, you can get the crucial quantitation data you need, without wasting your sample.
     


Quant-iT assay kits and reagents for use with microplate readers*—for 20–2,000 samples

Product

Target

Useful range†

Ex/Em‡

No. of assays

Cat. No.

Quant-iT 1X dsDNA HS AssaydsDNA 0.2–100 ng 510/527 1,000 Q33232
Quant-iT dsDNA High-Sensitivity (HS) Assay KitdsDNA 0.2–100 ng 510/527 1,000 Q33120
Quant-iT 1X dsDNA BR AssaydsDNA 4-4,000 ng 510/527 1,000 Q33267
Quant-iT dsDNA Broad-Range (BR) Assay KitdsDNA 4–1,000 ng 510/527 1,000 Q33130
Quant-iT RNA Assay Kit
Quant-iT RNA Assay Reagent
RNA 5–100 ng 644/673 1,000 Q33140
Q32884
Quant-iT RNA Broad-Range BR Assay KitRNA 20–1,000 ng 644/673 1,000 Q10213
Quant-iT RNA XR Assay KitRNA 200–10,000 ng 644/673 1,000 Q33225
Quant-iT microRNA Assay KitmicroRNA 1–100 ng 500/525 1,000 Q32882
Quant-iT Protein Assay KitProtein 0.25–5 µg 470/570 1,000 Q33210
Quant-iT Endotoxin Detection Assay KitEndotoxin 0.01–10.0 EU/mL 490/525 160 Q32892


Quant-iT bulk reagents and kits for >2,000 samples

Product

Target

Useful range†

Ex/Em‡

No. of assays

Cat. No.

Quant-iT PicoGreen dsDNA Assay Kit (1mL)
Quant-iT PicoGreen dsDNA Reagent
(1mL)
dsDNA 50 pg–2 µg 502/523 2,000 P7589
P7581
Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL)
Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL)
dsDNA 50 pg–2 µg 502/523 2,000 P11496
P11495
Quant-iT OliGreen ssDNA Assay Kit
Quant-iT OliGreen ssDNA Reagent
ssDNA 200 pg–2 µg 500/525 2,000 O11492
O7582
Quant-iT RiboGreen RNA Assay Kit
Quant-iT RiboGreen RNA Reagent
RNA 1–200 ng 500/525 2,000 R11490
R11491
RediPlate 96 RiboGreen RNA Quantitation KitRNA 3–200 ng 500/525 96 R32700

* Invitrogen Quant-iT assays and reagents can also be used with the Invitrogen Qubit Fluorometer. †The useful range assumes a 1–20 µL sample volume in a 96-well microplate assay. ‡ Excitation (Ex) and emission (Em), in nm, of the Quant-iT detection reagent. 
 

Expertly detect fluorescence with Thermo Scientific plate readers

High-sensitivity fluorescence detection of nucleic acids or protein for 6–1,536 samples can be quickly performed on the Varioskan LUX Multimode Microplate Reader using Invitrogen reagents for accurate and precise detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.

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Choosing the right technology for your samples and workflow can save significant time and money by helping to prevent downstream experimental failures.

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For Research Use Only. Not for use in diagnostic procedures.