NovaFluor Red 660 dye

NovaFluor dyes, including NovaFluor Red 660 dye, are built using Phiton technology and are compatible with both spectral flow cytometry, as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Red 660 dye has excitation and emission spectra similar to those of allophycocyanin (APC) and Alexa Fluor 647 dye. When excited by the red 640 nm laser line, the spectral spread of NovaFluor Red 660 is about half that of APC while maintaining similar brightness. The macromolecule-based NovaFluor Red 660 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Red 660 dye:

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Can serve as a replacement for APC with a cleaner emission spectrum, reducing spectral spillover for better marker resolution
  • Can be used in a flow cytometry panel with NovaFluor Red 685 or NovaFluor Red 700 dye
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Red 660 dye dashboard


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NovaFluor Red 660 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

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Experimental data using NovaFluor Red 660 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Red 660 dye.

Figure 2. Spectral signature of NovaFluor Red 660 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Red 660 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Side scatter plot with unstained and CD19 NovaFluor Red 660 dye-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Red 660 (Cat. No. H004T03R01) (right). Data was acquired in the R2 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.