NovaFluor Yellow 730 dye

NovaFluor dyes, including NovaFluor Yellow 730 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral interference for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Yellow 730 dye can be used as an alternative to PE-Cyanine 5.5, with far less cross-laser excitation and spectral spillover. NovaFluor Yellow 690, NovaFluor Yellow 700 and NovaFluor 730 dyes cannot be used together with conventional flow cytometry, but their distinct spectral signatures allow them to be successfully unmixed using a spectral flow cytometer. The macromolecule-based NovaFluor Yellow 700 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Yellow 730 dye

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Can serve as a replacement for PE-Cy5.5, with far less cross-laser excitation and spectral spillover
  • Can be used together with NovaFluor Yellow 690 and NovaFluor Yellow 700 using a spectral flow cytometer
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Yellow 730 dye dashboard


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NovaFluor Yellow 730 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

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Experimental data using NovaFluor Yellow 730 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 730 dye.
 

Figure 2. Spectral signature of NovaFluor Yellow 730 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 730 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
 

Side scatter plot with unstained and CD4 NovaFluor Yellow 730 dye stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD4 Monoclonal Antibody, NovaFluor Yellow 730 (Cat. No.  H001T03Y07) (right). Data was acquired in the YG7 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.