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NovaFluor dyes, including NovaFluor Red 685 dye, are built using Phiton technology and are compatible with both spectral flow cytometry, as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.
NovaFluor Red 685 dye is ~4 times brighter than APC-Cyanine 5.5 and Alexa Fluor 680 dye. In complex multicolor flow cytometry panels, NovaFluor Red 685 dye can be used to fill the spectral gap between Alexa Fluor 647 and Alexa Fluor 700 dyes. The macromolecule-based NovaFluor Red 685 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.
Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
Figure 1. Absorption and fluorescence emission spectra of NovaFluor Red 685 dye.
Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD16 Monoclonal Antibody, NovaFluor Red 685 (Cat. No. H006T03R02) (right). Data was acquired in the R3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.