NovaFluor Red 710 dye

NovaFluor dyes, including NovaFluor Red 710 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, which helps reduce spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Red 710 dye can be used as an alternative to Alexa Fluor 700 and APC-R700, with far less cross-laser excitation and spectral spillover using the 640nm laser for excitation. NovaFluor Red 710 is a brighter alternative to Alexa Fluor 700 with a cleaner emission spectra. The macromolecule-based NovaFluor Red 710 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Red 710 dye

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Bright fluorescence allows it to be paired with medium-to-low abundance antigens
  • Replacement for Alexa Fluor 700 or APC-R700 with far less cross-laser excitation and spectral spillover
  • Brighter alternative to Alexa Fluor 700 with cleaner emission spectra
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Red 710 dye dashboard


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Photostability in buffer
 
 
 
 
 
640639710 

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NovaFluor Red 710 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.  

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Experimental data using NovaFluor Red 710 dye products
 

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Red 710 dye. 

Graph of fluorescence intensity vs channel for NovaFluor Red 710 dye
Figure 2. Spectral signature of NovaFluor Red 710 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Red 710 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Side scatter plot with unstained and CD4 NovaFluor Red 710 stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD4 Monoclonal Antibody, NovaFluor Red 710 (Cat. No.  H001T03R04) (right). Data was acquired in the R4 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.