Product box and its contents: 5 tubes of ElectroMAX DH10B Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium

什么是 DH10B 感受态细胞?

大肠杆菌 DH10B 菌株 由于其具有高转化效率,可摄取和容纳大质粒 (>30 kb) ,缺乏甲基化依赖性限制性系统 (MDRS) ,以及可进行蓝白斑菌落筛选,成为日常分子克隆应用的常用菌株。DH10B α 可摄入基因组 DNA ,是其与 DH5 α 菌株的主要区别。完整的 DH10B 基因组已被 Durfeet 等人测序并于 2008 年发布 [1]。

DH10B 感受态细胞的优势

  • 基因组突变使其成为 cDNA 和基因组 DNA 文库转化的理想选择
  • 蓝白斑筛选,便于重组 DNA 菌落鉴定
  • 有化学或电转感受态可供选择

哪种DH10B感受态细胞适合您?

 MAX Efficiency DH10B 感受态细胞
MAX Efficiency DH10B T1 噬菌体抗性感受态细胞*ElectroMAX DH10B 细胞ElectroMAX DH10B T1 噬菌体抗性感受态细胞
MegaXDH10B T1R 电转感受态细胞
 
Product box and its contents: 5 tubes of MAX Efficiency DH10B Competent Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 21 tubes of One Shot MAX Efficiency DH10B T1 Phage-Resistant Cells, 1 vial of pUC19 DNA, and 1 bottle of SOC medium
Product box and its contents: 5 tubes of ElectroMAX DH10B Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 5 tubes of ElectroMAX DH10B T1 Phage-Resistant Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 5 tubes of MegaX DH10B T1R Electrocomp Cells, 1 vial of pUC19 DNA, and 3 bottles of recovery media
适用于
  • 应用广泛的高效感受态细胞
  • 应用广泛
  • 方便的单次使用或高通量形式
  • 高转化效率
  • 文库构建和其他具有挑战性的实验应用 
  • 高转化效率
  • 文库构建和其他具有挑战性的实验应用
  • 更高的转化效率
  • 高要求的分子克隆
  • 文库构建
转化效率>1 x 109 cfu/µg>1 x 109 cfu/µg>1 x 1010 cfu/µg>1 x 1010 cfu/µg>3 x 1010 cfu/µg
转化类型化学修饰化学修饰电穿孔电穿孔电穿孔
抗噬菌体 T1 、 T5 和 φ80 γ 污染––
可提供的规格

* 菌株也可以高通量的 FlexPlate 形式 提供。

参考文献

Competent cells: Featured products

In addition to competent cells, we offer a broad suite of products for the cloning workflow.

Resources

Have any questions on competent cells or transformation? Click on the resources listed below to access overviews, videos, genotype guides, and educational resources.

Icon with monitor with speech bubble onscreen.

Stay up-to-date on industry trends in molecular cloning with on-demand webinars.

Icon with two sheets of paper

Short articles and digital handbook for cloning applications

Icon of mouse inside a circle

Get support for your cloning experiments

仅供科研使用,不可用于诊断目的。

Stylesheet for Classic Wide Template adjustments