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E-Gel DNA Ladder 和 E-Gel 上样缓冲液,用于 琼脂糖凝胶电泳 |
Invitrogen E-Gel DNA Ladder 是具有特定质量和大小的双链 DNA 片段,与上样缓冲液预混合。这些 Ladder经特殊优化,在 Invitrogen E-Gel 预制琼脂糖凝胶中具有出色性能。为获得理想的电泳结果,建议与Invitrogen E-Gel 上样缓冲液一同使用。
选择使用适合特定实验应用的 DNA Ladder 进行准确的条带分析。表 1 列出了从高到低 bp 范围内可选的 E-Gel DNA Ladder。为获得理想性能,建议使用表 2中推荐的 E-Gel DNA Ladder 和 E-Gel 预制琼脂糖凝胶组合。
E-Gel 96 High Range DNA Marker | E-Gel 1 Kb Plus DNA Ladder | E-Gel 1 Kb Plus Express DNA Ladder | E-Gel Low Range Quantitative DNA Ladder | E-Gel 50 bp DNA Ladder | E-Gel Sizing DNA Ladder | E-Gel Ultra Low Range DNA Ladder |
---|---|---|---|---|---|---|
400-10,000 bp | 100-15,000 bp | 100-5,000 bp | 100-2,000 bp | 50-2,500 bp | 50-1,500 bp | 10-300 bp |
E-Gel 1 Kb Plus DNA Ladder | E-Gel 1 Kb Plus Express DNA Ladder | E-Gel 50 bp DNA Ladder | E-Gel 96 High Range DNA Marker | E-Gel Low Range Quantitative DNA Ladder | E-Gel Ultra Low DNA Ladder | Millennium RNA Marker | Century-Plus RNA Ladder | E-Gel Sizing DNA Ladder | |
---|---|---|---|---|---|---|---|---|---|
E-Gel Agarose Gels with SYBR Safe DNA Stain, 1% | ✓ | ||||||||
E-Gel Agarose Gels with SYBR Safe DNA Stain, 2% | ✓ | ||||||||
E-Gel Agarose Gels with SYBR Safe DNA Stain, 4% | ✓ | ✓ | |||||||
E-Gel Double Comb Agarose Gels with SYBR Safe DNA Gel Stain, 1% | ✓ | ✓ | |||||||
E-Gel Double Comb Agarose Gels with SYBR Safe DNA Gel Stain, 2% | ✓ | ✓ | |||||||
E-Gel EX Gel, 1% | ✓ | ✓ | |||||||
E-Gel EX Gel, 2% | ✓ | ✓ | ✓ | ✓ | |||||
E-Gel EX Gel, 4% | ✓ | ✓ | ✓ | ||||||
E-Gel EX Double Comb Agarose Gels, 1% | ✓ | ✓ | |||||||
E-Gel EX Double Comb Agarose Gels, 2% | ✓ | ✓ | |||||||
E-Gel Go! Agarose Gels, 1% | ✓ | ✓ | ✓ | ||||||
E-Gel Go! Agarose Gels, 2% | ✓ | ✓ | |||||||
E-Gel CloneWell II | ✓ | ||||||||
E-Gel SizeSelect II | ✓ | ||||||||
E-Gel NGS | ✓ | ✓ | |||||||
E-Gel 48-well, 1% | ✓ | ✓ | ✓ | ✓ | |||||
E-Gel 48-well, 2% | ✓ | ✓ | ✓ | ||||||
E-Gel 48-well, 4% | ✓ | ✓ | |||||||
E-Gel 96-well, 1% | ✓ | ||||||||
E-Gel 96-well, 2% | ✓ |
E-Gel 上样缓冲液 是即用型缓冲液,无需进一步稀释。该缓冲液含有独特的示踪染料二甲苯青FF(xylene cyanol FF,以下简称 XCFF)和柠檬黄;这些染料在电泳过程中可对 DNA 迁移进行视觉示踪,并指示何时达到理想分离结果。
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查找适合您实验应用的 E-Gel 琼脂糖预制胶和 DNA Ladder。
查看琼脂糖凝胶电泳和聚丙烯酰胺凝胶电泳的5大主要步骤,实现核酸样品的最佳分离和分析
仅供科研使用,不可用于诊断目的。