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Array-based comparative genomic hybridization (aCGH) has emerged as a powerful tool for detecting gene copy number variants implicated in many disease states. Simple, robust genomic DNA labeling and removal of free dye and nucleotides are two of the most critical components determining aCGH experiment’s performance. Suboptimal labeling and purification can lead to lower signals, channel bias, and greater variation, decreasing your ability to make accurate calls from array data. The BioPrime Total Genomic Labeling System reduces labeling variability
and increases signal-to-noise ratios on arrays, by using an optimized dye-labeled nucleotide mix with novel, application-specific Alexa Fluor 3 and Alexa Fluor 5 dyes, improved dNTP linkers, and new buffer chemistry. You’ll experience consistently higher DNA yields and improved signal-to-background ratios on arrays for more accurate data interpretation.
Figure 2. The BioPrime Total Genomic labeling System provides significantly higher yields of DNA compared to competitive kits. Each labeling reaction started with 1ug of sonicated DNA and followed the standard conditions per manufacture's recommendations. labeling reactions using ENZO Genomic DNA Labeling Kit or the BioPrime Array CGH Genomic Labeling System with GE Amersham Cy dyes were purified with QIAQuick columns (Qiagen). Labeled DNA from the BioPrime Total Genomic labeling System was purified using the included Purelink purification columns.
Figure 3. The BioPrime Total Genomic Labeling Systme provides better dye incorporation for more even signal on arrays. Each labeling reaction started with 1ug of sonicated DNA and followed the standard conditions per manufacture's recommendations. Labeling reactions using the ENZO Genomic DNA Labeling Kit or the BioPrime Array CGH Genomic Labeling System with GE Amersham Cy dyes were purified with QIAQuick columns (Qiagen). Labeled DNA from the BioPrime Total Genomic labeling System was purified using the included Purelink™ purification columns.
The BioPrime Total Genomic Labeling System includes all the components necessary to complete sample labeling and clean-up of your aCGH samples. There’s no need to qualify reagents sourced from multiple vendors, saving you time, effort, and resources. Each kit manufacturing lot is quality controlled as a complete solution to assure optimal performance in your hands. The labeling module features Alexa Fluor 3 and Alexa Fluor 5 supermix formulations that include dye-labeled dNTPs. This streamlines reaction setup, which minimizes any potential variation. Labeling takes just three simple steps. Labeling and clean-up take approximately 2.5 hours from start to finish. For your convenience, BioPrime Total Genomic Labeling products are available in two formats. The all-inclusive BioPrime Total Genomic Labeling System contains both labeling and purification modules. The BioPrime Total Genomic Labeling Module is also sold separately. The labeling module includes Alexa Fluor 3 and Alexa Fluor 5 reaction mixes, Exo– Klenow fragment, TE buffer, control DNA, and EDTA. The purification module provides PureLink™ spin columns and collection tubes; binding, wash, and elution buffers; and recovery tubes.
Figure 4. Overview of BioPrime Total Genomic Labeling System labeling protocol. BioPrime Total Genomic Labeling System features two dNTP supermix formulations that include dye-labeled nucleotides. This reduces the number of pipetting steps, increase labeling consistency, and simplifies the workflow.