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Microarray Labeling Kits and Components for Array Genomic Profiling
More Correct Calls With Higher Signal-to-Noise Ratios
Life Technologies BioPrime labeling systems enable reliable and consistent genomic DNA labeling and efficient removal of free dye and nucleotides. And because results obtained using these reagents exhibit improved signal-to-background and reduced channel bias, you can count on greater accuracy.
| Sample Type | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Platform | gDNA | ChiP* | FFPE | DNA Input Amount | Labeling Method | Label | Master Mix | Product | |
| Non-Platform Specific |  | | 5 ng–3 μg | Direct | AlexaFluor 3 AlexaFluor 5 (included) | | BioPrime Total Genomic Labeling System 18097011 - (30 reactions, w/ purification) 18097012 - (30 reactions, w/o purification) 18097010 - (10 reactions, w/ purification) | ||
| | 250 ng–1 μg (Bac arrays) 4 μg (cDNA arrays) | Direct | User’s choice (not included) | BioPrime aCGH Genomic Labeling System 18095011 - (30 reactions, w/ purification) 18095012 - (30 reactions, w/o purification) | ||||
| Array-dependent | Direct | AlexaFluor 3 AlexaFluor 5 (included) | BioPrime Total FFPE Genomic Labeling System A10965011 - (30 reactions, w/ purification) | |||||
| * ChIP must first be performed using a kit such as the MAGnify™ Chromatin Immunoprecipitation System, Cat. No. 492024 | |||||||||
Repetitive Sequence Blocking Reagents
| Product | Size | Cat. No. |
|---|---|---|
| Human Cot-1 DNA-Fluorometric QC | 1 mg | 15729101 |
| Human Cot-1 DNA-Fluorometric QC | 500 μg | 15279011 |
| Mouse Cot-1 DNA | 500 μg | 18440016 |
| Yeast tRNA | 25 mg | 15401011 |
Which BioPrime system is right for me?
The BioPrime Total aCGH reagents offer various configurations to allow for optimal performance on arrays based on premier enzymes, primers, dyes, and purification systems. For two-color array CGH and ChIP-on-chip experiments, we recommend the BioPrime Total Genomic Labeling system. The BioPrime Total Genomic Labeling system allows for the lowest starting input material, includes application-optimized dye-labeled nucleotides (with Alexa Fluor 3 and Alexa Fluor 5 dyes), and is the simplest, easiest-to-use kit in the product family. For researchers who wish to use other dyes, we offer BioPrime aCGH Genomic Labeling systems without dyes—this allows you to take advantage of the higher-performance enzymes and reagents in our BioPrime kits to deliver superior yield and better total dye incorporation.