Workflow Features

  • Ideal for quantitating small RNAs - all you need is the sequence of interest
  • Analyze any small nucleic acid less than 200 bases long, including newly discovered miRNAs, Piwi-interacting RNA (piRNA), small nuclear RNA (snRNA), and small nucleolar RNA (snoRNA)
  • Assays include both the Custom TaqMan Small RNA Assay and a target-specific reverse transcription primer

See Literature/References

它如何工作

Custom TaqMan Small RNA Assays employ an innovative target-specific stem-loop reverse transcription primer to address the challenge of the short length of the small RNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan Assay-based qPCR. Also, the stem-loop structure in the tail of the primer confers a key advantage if you are studying miRNAs: specific detection of the mature, biologically active miRNA.

Small RNA Analysis Experimental Workflow

Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs. Each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

推荐产品

 

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.

 

RecoverAll™ 针对FFPE的总核酸分离试剂盒
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

 

TaqMan® microRNA Cells-to-CT™试剂盒
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

Two-step TaqMan® MicroRNA Assays, containing the TaqMan® Assay and reverse transcription primer specific for the target of interest, are designed for individual miRNA targets found in a wide variety of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. The TaqMan® MicroRNA Reverese Transcription Kit provides optimal TaqMan® MicroRNA Assay performance.

推荐产品

 

TaqMan® MicroRNA反转录试剂盒
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

 

定制TaqMan®小RNA分析
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

For the final quantitation step, the cDNA product generated in step 2 is mixed with TaqMan® Universal Master Mix II and added to the matched TaqMan® MicroRNA Assay.

推荐产品

 

定制TaqMan®小RNA分析
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

 

TaqMan®通用预混液II
This kit contains components needed for all 5' nuclease assays using TaqMan® Assay-based PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Integromics RealTime StatMiner® Software is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. The software easily imports raw data or Ct values from any Applied Biosystems® Real-Time PCR System and guides you though every step of analysis for accurate results in minutes.

推荐产品

 

RealTime StatMiner® Software (optional)
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you through every step of analysis, giving you accurate results in minutes.

Step 1 : Isolate RNA

Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs. Each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

推荐产品

 

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.

 

RecoverAll™ 针对FFPE的总核酸分离试剂盒
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

 

TaqMan® microRNA Cells-to-CT™试剂盒
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

Step 2 : Reverse Transcribe RNA into cDNA

Two-step TaqMan® MicroRNA Assays, containing the TaqMan® Assay and reverse transcription primer specific for the target of interest, are designed for individual miRNA targets found in a wide variety of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. The TaqMan® MicroRNA Reverese Transcription Kit provides optimal TaqMan® MicroRNA Assay performance.

推荐产品

 

TaqMan® MicroRNA反转录试剂盒
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

 

定制TaqMan®小RNA分析
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

Step 3 : Set Up & Run qPCR Reactions

For the final quantitation step, the cDNA product generated in step 2 is mixed with TaqMan® Universal Master Mix II and added to the matched TaqMan® MicroRNA Assay.

推荐产品

 

定制TaqMan®小RNA分析
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

 

TaqMan®通用预混液II
This kit contains components needed for all 5' nuclease assays using TaqMan® Assay-based PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Step 4 : Analyze Data

Integromics RealTime StatMiner® Software is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. The software easily imports raw data or Ct values from any Applied Biosystems® Real-Time PCR System and guides you though every step of analysis for accurate results in minutes.

推荐产品

 

RealTime StatMiner® Software (optional)
RealTime StatMiner® Software from Integromics is a powerful, easy-to-use bioinformatics tool for quality control and differential expression analysis of real-time PCR data. With just a single click, the software imports raw data or Ct values from any of our real-time PCR instruments, and then guides you through every step of analysis, giving you accurate results in minutes.

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