Proteases and Protein-Cleaving Reagents | Thermo Fisher Scientific

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We offer purified and agarose-immobilized proteases for enzymatic proteolysis (cleavage or digestion) of proteins to facilitate amino acid sequencing, peptide analysis, and polypeptide structural characterization.

For protein digestion using mass spectrometry, we offer a wide range of highly purified and MS-validated proteases.

Choose the right protease for your application

Fusion tag removal

Enzyme	Purified protease	Immobilized proteases	Specificity
Factor Xa	32521 32520	N/A	Cleaves at the carboxyl side of Ile-Glu-Gly-Arg sequence
Human Rhinovirus 3C Protease (HRV 3C)	88946	N/A	Highly specific for the recognition sequence Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro and cleaves after the glutamine residue
S. aureus V-8 Protease	20195	20151	Cleaves at the carboxyl side of aspartate and glutamate residues

Antibody fragmentation

Enzyme	Purified protease	Immobilized proteases	Specificity
Ficin	N/A	44881	Cleaves non-specifically at exposed residues, favors aromatic residues
Papain	N/A	20341	Cleaves nonspecifically at exposed residues
Pepsin	N/A	20343	Cleaves nonspecifically at exposed residues, favors aromatic residues

Proteomics using MS Analysis

Enzyme	Purified protease	Immobilized proteases	Specificity
Trypsin, MS Grade	90057 90058 90059 90305	N/A	Cleaves at the carboxyl side of arginine and lysine residues
Lys-C Protease, MS Grade	90051	N/A	Cleaves at the carboxyl side of lysine residues
Trypsin/Lys-C Protease Mix, MS Grade	A41007	N/A	Cleaves at the carboxyl side of lysine residues
Lys-N Protease, MS Grade	90300 90301	N/A	Cleaves at the amino side of lysine residues
Asp-N Protease, MS Grade	90053	N/A	Cleaves at the amino side of aspartate residues
Chymotrypsin, MS Grade	90056	N/A	Cleaves at the carboxyl side of tyrosine, phenylalanine, tryptophan, and leucine
Glu-C Protease, MS Grade	90054	N/A	Cleaves at the carboxyl side of glutamate or aspartate

Adherent cells dissociation

Enzyme	Purified protease	Immobilized proteases	Specificity
Trypsin (TPCK)	20233	20230	Cleaves at the carboxyl side of arginine and lysine residues

DNase/RNase deactivation

Enzyme	Purified protease	Immobilized proteases	Specificity
Proteinase K	17916	N/A	Cleaves at the carboxyl side of aliphatic, aromatic, and hydrophobic residues

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